##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_1_1.341000000097d4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66052 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.950463271361954 38.0 38.0 38.0 32.0 38.0 2 36.97548900865984 38.0 38.0 38.0 32.0 38.0 3 36.92077454127051 38.0 38.0 38.0 32.0 38.0 4 37.01035547750257 38.0 38.0 38.0 32.0 38.0 5 37.023314963967785 38.0 38.0 38.0 32.0 38.0 6 38.99442863198692 40.0 40.0 40.0 38.0 40.0 7 38.888421243868464 40.0 38.0 40.0 38.0 40.0 8 38.90009386543935 40.0 38.0 40.0 38.0 40.0 9 38.88347059892206 40.0 38.0 40.0 38.0 40.0 10 38.8272875915945 40.0 38.0 40.0 38.0 40.0 11 38.828165687640045 40.0 38.0 40.0 38.0 40.0 12 38.805910494761704 40.0 38.0 40.0 38.0 40.0 13 38.765836007993705 40.0 38.0 40.0 38.0 40.0 14 38.77102888633198 40.0 38.0 40.0 38.0 40.0 15 38.827635802095315 40.0 38.0 40.0 38.0 40.0 16 38.84692363592321 40.0 38.0 40.0 38.0 40.0 17 38.83771876703204 40.0 38.0 40.0 38.0 40.0 18 38.8547659419851 40.0 38.0 40.0 38.0 40.0 19 38.87673348271059 40.0 38.0 40.0 38.0 40.0 20 38.88228971113668 40.0 38.0 40.0 38.0 40.0 21 38.88951129413189 40.0 38.0 40.0 38.0 40.0 22 38.848937200993156 40.0 38.0 40.0 38.0 40.0 23 38.85043602010537 40.0 38.0 40.0 38.0 40.0 24 38.86677163446981 40.0 38.0 40.0 38.0 40.0 25 38.88012475019681 40.0 38.0 40.0 38.0 40.0 26 38.84674196087931 40.0 38.0 40.0 38.0 40.0 27 38.8311179071035 40.0 38.0 40.0 38.0 40.0 28 38.8024889481015 40.0 38.0 40.0 38.0 40.0 29 38.667504390480225 40.0 38.0 40.0 38.0 40.0 30 38.66511233573548 40.0 38.0 40.0 38.0 40.0 31 38.604145218918426 40.0 38.0 40.0 38.0 40.0 32 38.519060740023015 40.0 38.0 40.0 38.0 40.0 33 38.409207896808574 40.0 38.0 40.0 38.0 40.0 34 38.2765245564101 40.0 38.0 40.0 38.0 40.0 35 38.19714770181069 40.0 38.0 40.0 38.0 40.0 36 38.03827287591594 40.0 38.0 40.0 38.0 40.0 37 37.95912311512142 40.0 38.0 40.0 38.0 40.0 38 37.855977108944465 40.0 38.0 40.0 38.0 40.0 39 37.70294616362865 40.0 38.0 40.0 32.0 40.0 40 37.54105855992248 40.0 38.0 40.0 32.0 40.0 41 37.39792890449949 40.0 38.0 40.0 32.0 40.0 42 37.25127172530733 40.0 38.0 40.0 32.0 40.0 43 37.07495609519772 40.0 38.0 40.0 32.0 40.0 44 36.867801126385274 40.0 38.0 40.0 27.0 40.0 45 36.623614727790226 40.0 38.0 40.0 27.0 40.0 46 36.39411372857749 40.0 38.0 40.0 27.0 40.0 47 36.068234118573244 40.0 38.0 40.0 13.0 40.0 48 35.80615272815358 40.0 38.0 40.0 13.0 40.0 49 35.52516199358082 40.0 38.0 40.0 13.0 40.0 50 35.27806879428329 40.0 38.0 40.0 13.0 40.0 51 34.74345969841943 40.0 38.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.4186538119618888 2201 2 0.014757642831135342 2201 3 0.10187248837421947 2201 4 0.48687743475275624 2201 5 0.4760643905742583 2201 6 0.6710362624382356 2201 7 0.2378055050720107 2201 8 0.47819541705817414 2201 9 0.3646539015488415 2201 10 0.3852419405766909 2201 11 0.06593360198504428 2201 12 0.5044670521076284 2201 13 0.778617513391552 2201 14 0.6194305430630322 2201 15 0.6936078407442352 2201 16 0.650583907430665 2201 17 0.7495302468105933 2201 18 0.7109662117335134 2201 19 0.529929087315125 2201 20 0.4460947611484869 2201 21 0.6887584189322951 2201 22 0.6436264924789512 2201 23 0.6101009939242488 2201 24 0.5482551124001915 2201 25 0.6963585680454969 2201 26 0.2696164699921795 2201 27 0.2879986275617554 2201 28 0.5060957818085257 2201 29 0.8077797240893716 2201 30 0.9273977044855712 2201 31 0.5825398123762611 2201 32 -0.008734306790572077 2201 33 -0.01701386287879103 2201 34 0.09512704277385353 2201 35 0.4026640741590697 2201 36 0.345280508903393 2201 37 0.2564126661252004 2201 38 0.38804521288687965 2201 39 0.006040424012383028 2201 40 0.2312371356127514 2201 41 0.5272995090957835 2201 42 0.1933467153735151 2201 43 0.3443640337308409 2201 44 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-1.7190491923573603 1213 50 -0.7541761118705921 1213 51 -1.1068245903614198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 5.0 16 10.0 17 5.0 18 2.0 19 12.0 20 8.0 21 6.0 22 19.0 23 39.0 24 73.0 25 115.0 26 214.0 27 274.0 28 446.0 29 641.0 30 869.0 31 1121.0 32 1391.0 33 1846.0 34 2116.0 35 2482.0 36 3280.0 37 4533.0 38 7922.0 39 38622.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.18496619060614 16.34221777494769 12.222929743169896 21.249886291276265 2 16.82765094168231 21.81614485556834 38.966269000181676 22.389935202567674 3 21.6026766789802 28.085447828983224 27.599467086537878 22.7124084054987 4 13.133591715617998 16.085811179071037 36.312299400472355 34.46829770483861 5 16.605099012898926 36.07914976079453 30.030884757463816 17.28486646884273 6 30.477502573729787 36.22146187851995 14.638466662629442 18.662568885120816 7 28.725852358747655 31.96421001635075 20.48234724156725 18.827590383334343 8 28.742505904438925 30.351844001695632 18.190213770968327 22.715436322897112 9 28.518440016956337 14.421970568642886 20.909283594743535 36.15030581965724 10 21.439169139465875 25.1816750439048 26.819778356446438 26.559377460182887 11 33.140555925634345 23.878156603887845 22.886513655907468 20.09477381457034 12 27.00902319384727 25.030279173984134 28.59111003451826 19.369587597650337 13 30.89232725731242 22.504996063707384 26.152122570096285 20.45055410888391 14 23.754011990552897 20.48688911766487 29.84769575485981 25.911403136922424 15 24.070429358687097 26.28837885302489 27.290619511899717 22.3505722763883 16 21.20450554108884 27.991582389632413 27.44807121661721 23.35584085266154 17 21.773754011990555 25.672197662447772 26.816750439048022 25.737297886513655 18 23.852419306001334 23.5965602858354 30.388179010476595 22.162841397686673 19 26.513958699206686 23.295282504693272 30.59104947617029 19.599709319929755 20 24.19154605462363 22.280930176224793 30.709138254708414 22.818385514443165 21 24.47768424877369 23.53297402046872 31.635680978622904 20.35366075213468 22 22.520135650699448 24.047719978198995 31.255677345122024 22.176467025979534 23 21.97056864288742 25.720644340822385 32.36389511294132 19.94489190334888 24 22.255192878338278 25.71610246472476 31.809786229031673 20.218918427905287 25 20.583782474414097 27.131653848483012 32.6076424635136 19.676921213589292 26 20.143220492944952 27.422333918730697 32.2685157148913 20.165929873433054 27 20.70187125295222 27.660025434506146 31.57663658935384 20.06146672318779 28 19.935808151153637 27.59492521044026 30.84842245503543 21.62084418337068 29 19.926724398958395 28.653182341185733 30.429055895355173 20.9910373645007 30 19.996366499121905 28.255011203294373 30.933204142191002 20.81541815539272 31 20.270393023678317 27.626718343123603 30.680373039423486 21.4225155937746 32 20.85780899897051 27.043844243929026 30.681886998122692 21.416459758977773 33 20.33700720644341 28.09604553987767 30.665233452431416 20.901713801247503 34 20.75183189002604 28.5547750257373 29.546417973717677 21.146975110518984 35 20.533821837340277 29.20274934899776 29.70235571973597 20.561073093925998 36 20.320353660752136 29.34354750802398 30.241325016653548 20.09477381457034 37 20.533821837340277 30.0051474595773 29.29358687095016 20.16744383213226 38 20.02967359050445 30.15351541209956 29.749288439411377 20.067522557984617 39 20.111427360261615 30.02937079876461 29.693271967540724 20.165929873433054 40 20.1083994428632 30.2988554472234 29.41318960818749 20.17955550172591 41 20.258281354084662 29.83709804396536 28.92115303094532 20.983467571004663 42 20.5383637134379 30.076303518440017 28.056682613698296 21.328650154423787 43 19.953975655544117 29.927935565917764 28.108157209471322 22.009931569066797 44 19.471022830497184 29.8688911766487 28.678919639072248 21.98116635378187 45 20.081148186277478 29.230000605583477 27.697874401986315 22.990976806152727 46 20.452068067583117 28.92115303094532 27.725125658572036 22.901653242899535 47 20.008478168715556 28.442742081996002 27.632774177920428 23.91600557136801 48 20.031187549203658 28.45182583419124 26.55786350148368 24.95912311512142 49 20.464179737176767 28.044570944104645 26.842487736934533 24.648761581784047 50 20.530793919941864 28.023375522315753 26.725912917095624 24.719917640646763 51 20.38696784351723 27.607036880033913 26.529098286198753 25.476896990250108 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 7.0 2 5.0 3 13.0 4 21.0 5 23.5 6 26.0 7 29.0 8 32.0 9 50.5 10 69.0 11 156.0 12 243.0 13 558.5 14 874.0 15 1208.5 16 1543.0 17 1647.5 18 1752.0 19 1717.5 20 1683.0 21 1605.0 22 1527.0 23 1659.5 24 1792.0 25 1886.5 26 1687.5 27 1394.0 28 1258.5 29 1123.0 30 1200.5 31 1278.0 32 1591.5 33 1905.0 34 1978.0 35 2051.0 36 1948.5 37 1846.0 38 1783.0 39 1720.0 40 1832.5 41 1945.0 42 2082.0 43 2219.0 44 2466.5 45 2714.0 46 2945.5 47 3177.0 48 3436.5 49 3696.0 50 3945.5 51 4195.0 52 4337.0 53 4479.0 54 4360.5 55 4242.0 56 3937.0 57 3632.0 58 3376.0 59 3120.0 60 2806.0 61 2492.0 62 2193.0 63 1894.0 64 1667.0 65 1440.0 66 1364.5 67 1289.0 68 1069.5 69 850.0 70 740.0 71 630.0 72 559.0 73 488.0 74 404.5 75 231.5 76 142.0 77 123.5 78 105.0 79 82.0 80 59.0 81 43.0 82 27.0 83 21.0 84 15.0 85 10.0 86 5.0 87 3.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14231211772542846 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 66052.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.56296161210922 #Duplication Level Percentage of deduplicated Percentage of total 1 88.23298760631069 65.78912867811052 2 7.8051415786190015 11.639489438072921 3 1.8189720789635304 4.068838358917686 4 0.7579578978073672 2.260623425512229 5 0.3589943880914065 1.3383842389111091 6 0.2506795279653524 1.1214844812373337 7 0.21954486760426753 1.1458940874718822 8 0.11967628477002482 0.7138734581749769 9 0.07437966135343109 0.4991371050795808 >10 0.3001639391979294 3.9806346429561663 >50 0.030752458320582314 1.5775564970362292 >100 0.028699730266781794 5.080821573798469 >500 0.0020499807296239783 0.7841340147208888 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 513 0.7766608126930298 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 419 0.6343486949676013 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 389 0.5889299339914007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.4874947011445528 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 321 0.4859807424453461 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 313 0.4738690728516926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 278 0.42088051837945856 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250 0.3784896748016714 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 224 0.3391267486222976 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 145 0.2195240113849694 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 145 0.2195240113849694 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 144 0.21801005268576273 RNA PCR Primer, Index 27 (95% over 21bp) ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 128 0.19378671349845578 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.19378671349845578 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 118 0.1786471265063889 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.1377702416278084 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 89 0.13474232422939503 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.12565857203415493 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 81 0.12263065463574153 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 73 0.11051898504208806 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 71 0.10749106764367468 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 69 0.1044631502452613 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.10294919154605464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0030279173984133715 19 0.0 0.0 0.004541876097620057 20 0.0 0.0 0.013625628292860171 21 0.0 0.0 0.0211954217888936 22 0.0 0.0 0.04541876097620057 23 0.0 0.0 0.07872585235874766 24 0.0 0.0 0.12111669593653485 25 0.0 0.0 0.1468539938230485 26 0.0 0.0 0.1665354569127354 27 0.0 0.0 0.18318900260400897 28 0.0 0.0 0.24677526797068977 29 0.0 0.0 0.41633864228183853 30 0.0 0.0 0.5753043056985405 31 0.0 0.0 0.7872585235874765 32 0.0 0.0 0.9447102283049719 33 0.0 0.0 1.1097317265185005 34 0.0 0.0 1.2792951008296494 35 0.0 0.0 1.4170653424574577 36 0.0 0.0 1.5911705928662265 37 0.0 0.0 1.8243202325440562 38 0.0 0.0 2.13316780718222 39 0.0 0.0 2.508629564585478 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTGT 25 0.002092793 36.0 6 GGAGTGG 35 2.7918804E-4 32.142857 6 ACGGGAG 55 4.0873765E-6 28.636362 3 CGGGAGT 55 4.0873765E-6 28.636362 4 GAGTGGT 40 6.1043235E-4 28.125002 7 GGGGCAG 95 7.0940587E-10 26.052631 5 ACGGGGC 100 1.2878445E-9 24.75 3 CGGGGCA 100 1.2878445E-9 24.75 4 GGGAGTG 65 4.1802492E-4 20.76923 5 GATACCA 55 0.0038979892 20.454544 1 ATACCAC 60 0.0064472416 18.750002 2 AACGCAG 920 0.0 17.853262 6 TACGGGA 115 3.350211E-6 17.608696 2 CGCAGAG 990 0.0 16.590908 8 ACGCAGA 1000 0.0 16.425001 7 CAGAGTA 1060 0.0 15.495283 10 AGAGTAC 1050 0.0 15.428572 11 GCAGAGT 1070 0.0 15.350467 9 ACGGGTG 90 0.0037042685 15.0 5 TACGGGT 110 8.438357E-4 14.318181 4 >>END_MODULE