FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_8.3410000000970d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_8.3410000000970d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544159
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA21540.3958401864161027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.3840789181103317No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14420.26499607651440116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.22989604141436604No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11820.21721592402220674No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9560.17568394531745318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.1710896998855114No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9190.16888446207817934No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7000.12863887209436947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC300.005145726729.999023
TGGACGC901.1885277E-824.99918230
TATGCCG551.3663608E-424.54690743
GACCTGC1700.022.49926438
TAGGTGG1257.4942363E-1021.5992959
TGTAATA654.235213E-420.7685532
ACCTGCA1800.019.99934639
GACGCTA1251.7627826E-819.79935332
AATATAG807.555552E-519.6868575
ATAGGTG1503.43789E-1019.4993638
AGGATAG1503.43789E-1019.4993635
CTGCACG1850.019.45882441
TGTCGGG1850.019.45882423
CACGAAT1757.2759576E-1219.28685644
GTATGCC706.978538E-419.28508442
TAACACA706.978538E-419.2850844
GCACGAA1901.8189894E-1218.9484943
TGCACGA1901.8189894E-1218.94674942
AACGCAG38900.018.8554256
CGGTTGT600.006507420418.74938838
Produced by FastQC (version 0.11.2)