FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_4.341000000096c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_4.341000000096c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086985
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.025.5510141
GCCCACG1159.094947E-1225.43389718
GTATCAA11800.022.7222921
CGTATGC1305.0931703E-1122.4992241
CCGAGCC1601.8189894E-1221.09301814
TATGCCG1653.6379788E-1220.45477743
GACCTAT1452.1827873E-1020.17171326
CGAGCCC1555.347829E-1018.87031215
CCCACGA1608.1672624E-1018.28061519
AGACCTA1507.2668627E-917.99937425
AACGCAG14700.017.6014296
TATCAAC15000.017.0994052
CAACGCA15150.016.9301055
TCAACGC15050.016.8930994
ACCTATT1601.6138074E-816.87441427
CGTCCTA952.8199263E-416.57837110
GTATGCC1803.9090082E-916.24943542
CGCAGAG15800.016.2336148
TCGTATG1956.475602E-1016.15328440
ACGCAGA16050.016.1209357
Produced by FastQC (version 0.11.2)