FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_3.341000000096bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_3.341000000096bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273977
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.22082145581563414No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG5120.18687700062413998No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5010.18286206506385574No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4900.17884712950357146No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.1773871529361954No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4540.16570734039718663No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.15512251028370996No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.14125273289363705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.12665296721987612No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3150.11497315468086736No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA2980.10876825426951897No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT2960.10803826598583093No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA2950.1076732718439869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.10256335385817057No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAG2800.10219835971632657No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC2790.10183336557448254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG207.0247136E-444.99890536
ATACTAT352.8152106E-432.1420756
TTAGGAC300.00514110929.9992713
TGTGTGA300.00514110929.99927110
TCGCGCG957.366907E-1026.0519989
CTAACGT500.00226056222.4994543
CGCGCGC1104.0199666E-922.49945310
ATACCCG1104.0199666E-922.4994535
TACCCGG1257.4578566E-1021.5994726
GTCGCGC1156.71389E-921.5212158
GGTCGCG1156.71389E-921.5212157
ACGTTAG754.548492E-521.022511
GCGCGTG754.58708E-520.9994898
GGCGCGT754.58708E-520.9994897
GCGGCCT654.2275822E-420.76872411
ACCTATA1201.0824806E-820.647111
ATTTTCG1201.0963049E-820.62449830
CGTCGGG1201.0963049E-820.62449814
GGGGCAA1351.9444997E-919.99951418
GGGGCTA1251.753142E-819.79951736
Produced by FastQC (version 0.11.2)