FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_2.341000000096af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_2.341000000096af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269568
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG3040.1127730294396961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGC856.4374035E-926.46971710
GGCTAGT957.348717E-1026.05177338
CCGGGAC450.001224355224.99917433
TCGCGCG901.1828888E-824.9991749
GTCGCGC1001.3333192E-924.7491868
CGGGGCA1301.8189894E-1224.2299717
ACCTATA751.6683916E-624.0348741
ATACCCG851.8830724E-723.8227445
TGATTCG1052.3464963E-923.5706545
GGGGCTA1052.3464963E-923.5706536
GCGCGCT1152.6193447E-1023.47748811
GCGGACC500.00226058222.4992622
TACACAG500.00226058222.499265
GGGCTAG1104.0199666E-922.49925837
GGTCGCG1104.0199666E-922.4992587
AGGTCGC1104.0199666E-922.4992586
TAGGCCG903.2686876E-722.4992565
CTAGTTG1156.71389E-921.5210340
GCTAGTT1156.71389E-921.5210339
TACCCGG855.01009E-621.1757746
Produced by FastQC (version 0.11.2)