FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_19.341000000097c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_19.341000000097c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714280
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG300.00510316730.0512871
GTATCAA13500.026.8792061
GGTATCA4500.025.5435921
CACCTAT551.3517297E-424.5874161
TATTAGG551.3678988E-424.5443842
GTCCTAT702.6657279E-522.5384641
TATCAAC17300.020.4181842
ACGGGGG3100.019.5959216
AACGCAG17900.019.3566996
CAACGCA18050.019.0711925
TCAACGC18050.019.0711924
CGCAGAG18150.018.9661188
ACGCAGA18450.018.7796697
ATCAACG18600.018.7491843
ACATGCG600.00650977718.7491828
CCCGAAC600.00650977718.74918213
TCCGGGC600.00650977718.74918232
CAGAGTA19400.018.09199510
GCAGAGT19350.018.0224729
TACTATA750.001109988417.9992162
Produced by FastQC (version 0.11.2)