FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_18.341000000097ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_18.341000000097ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770191
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8550.026.3525921
TAGGACG651.523277E-524.2300014
CTAGGTG500.002264354122.4992874
GGTATCA4150.022.2600041
GGTTATA854.9681294E-621.2060851
TAATACA654.2373882E-420.7685724
ATATACG550.003936120.4538974
TAGACAG1552.3646862E-1120.3219365
CGGGGGA1757.2759576E-1219.28510317
AACGCAG11350.019.2284646
TATCAAC11450.019.060532
CAACGCA11600.019.0080175
TCAACGC11800.018.876524
ACGGGGC1454.7675712E-918.62009816
AGAGTAC12000.018.56191311
CGGGGCA851.2040793E-418.52882417
CTAGGAC851.2040793E-418.5288243
ATCAACG11900.018.3397543
ACGCAGA11950.018.263027
CGCAGAG12000.018.1869248
Produced by FastQC (version 0.11.2)