FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_16.34100000009791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_16.34100000009791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001623
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11550.023.7998451
GCCCACG952.1154847E-823.68336918
TATGCCG1056.104892E-821.4288843
AGCCCGC1009.0556205E-720.2492829
GCGCTAA1009.0556205E-720.2492813
GGTATCA4700.019.6554161
GTATTAT1151.5712612E-719.5928881
TACCCGG1403.086825E-919.285036
CAACGCA14000.018.9636125
ACCTATA1555.2386895E-1018.8976551
AACGCAG14400.018.4368466
CTGCGCG1354.2722604E-818.3326829
TATACTG1354.2722604E-818.3326825
GCGCGCT2100.018.21363811
CGTATGC1253.8334292E-717.9993641
CGCGCTA1153.4489367E-617.6080712
CGCAGCG1153.4489367E-617.6080733
ACGCAGA15100.017.5821577
TCAACGC15100.017.4331554
CGTCGGG1551.08993845E-817.41873614
Produced by FastQC (version 0.11.2)