FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_15.34100000009784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_15.34100000009784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455281
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG6580.14452612781996174No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5310.11663126728328219No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4690.10301330387167484No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGAT4670.10257401472936493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGT250.002106279635.998244
TTTTAGA352.8185264E-432.1412923
CTCTCAT450.001210148825.0538121
GATTACG450.001210148825.0538121
ACGCTAG450.001225765824.9987833
GTCTACG1003.5503035E-822.5484311
TCTACGA1104.0381565E-922.4989032
CGGAACA500.002263167222.49890114
TAAAATC702.699381E-522.49890132
TCCCGGG500.002263167222.49890140
TATAGTG500.002263167222.4989013
GTATCAA22650.022.4986551
TACGAGT1156.7448127E-921.5206894
CTCCGAG955.519196E-721.31474945
GGACATA654.1718155E-420.8139361
ACATAGT654.2342086E-420.7682193
TACACAG654.2342086E-420.7682195
CTATCAG550.003884254520.4985751
GGAACAG550.003934054620.45354815
TACAGTA550.003934054620.45354837
Produced by FastQC (version 0.11.2)