FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_14.34100000009777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_14.34100000009777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173086
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24881.4374357255930579No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA24021.3877494424736836No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18141.048033925331916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.8816426516298257No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14850.8579550050264031No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14520.8388893382480386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.6927192262805774No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11080.6401442057705418No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8150.4708641946777902No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7280.4206001640802838No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.36860289104838057No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC5240.3027396785413032No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5220.30158418358503863No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.28425175924107093No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4840.27962977941601286No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4700.27154131472216125No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3580.20683359717134833No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.18776793039298384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3030.17505748587407416No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2980.17216874848341288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT2930.1692800110927516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2600.15021434431438707No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.1427036270986677No Hit
GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2470.1427036270986677No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.1375038997954774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.13519290988294835No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG2060.11901598049524513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2040.11786048553898062No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2020.11670499058271609No Hit
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA2020.11670499058271609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT2010.11612724310458385No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC2000.11554949562645159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT1960.11323850571392255No Hit
CAATTAGTACTGGTTAGCTTAACGCATTACTGCGCTTCCACACCCAGCCTA1860.10746103093259998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT1840.10630553597633545No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.1045722935419387No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1760.1016835561512774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT451.9130312E-839.99794837
ATAGACA552.7212081E-936.8162929
TAGACAA504.8403308E-835.99815430
CACGAGA504.8403308E-835.99815421
GAGACAT504.8403308E-835.99815424
AGACAAA504.8403308E-835.99815431
GACAAAT551.118824E-732.72559432
TATGCCG551.118824E-732.72559443
GTATGCC551.118824E-732.72559442
AGACATA551.118824E-732.72559425
ACATAGA551.118824E-732.72559427
GGAGTTT352.8113977E-432.1412112
ACAAATC602.400011E-729.99845933
CATAGAC602.400011E-729.99845928
CAAATCT602.400011E-729.99845934
TTAAAGG300.00513661529.9984593
GGACCTT300.00513661529.99845943
CCACGAG602.400011E-729.99845920
ATGCCGT654.835383E-727.69088444
AAATCTC709.2353184E-725.71296735
Produced by FastQC (version 0.11.2)