FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_13.3410000000975e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_13.3410000000975e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934545
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG709.306441E-725.7136445
CCGTGCA551.3681671E-424.5448429
ACCTATA2700.024.1932471
CGTATGC1204.5110937E-1022.49943741
TATGCCG1305.0931703E-1122.49943743
ATACCCG2900.022.4994375
TACCCGG2850.022.1047126
TCGCGCG1850.021.8913449
GCGTTAT1358.367351E-1121.66612632
ATGCCGT1358.367351E-1121.66612644
CGTTATC1401.364242E-1020.89233633
GGTATCA6400.020.0611041
GTATATA1703.6379788E-1219.8747771
CGTCGGG2950.019.83001514
GTATCAA14550.019.8155841
GCCTCGA3100.019.59628532
CGGGGCA3800.019.53898417
GTATTAT951.3029912E-518.9682081
TCGGCAG1901.8189894E-1218.94689634
TTCGGCA2050.018.65807233
Produced by FastQC (version 0.11.2)