FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_12.34100000009741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_12.34100000009741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458674
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.20188630705032332No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG9090.19817997095976664No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8430.18379066613760536No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7120.15523007626331556No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6410.13975067259099055No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.13430017834017188No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA6100.1329920597199754No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.12623344684896026No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5750.12536136776882928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.11860275489781412No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT4940.10770176639617682No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA4770.10399543030562011No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAAC4720.10290533145545638No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA4650.10137919306522716No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG4620.10072513375512893No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT4620.10072513375512893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG1450.032.5851829
GTCGCGC1600.029.5303218
GGTCGCG1600.029.5303217
CGCGCGC1800.028.74909610
GGCAGGT1550.024.67664336
TCGGCAG1650.024.54468234
TACCCGG2400.024.3742336
CGTCGGG2350.023.93541714
ATTCGGC1700.023.82277932
CGGCAGG1650.023.1810935
ACCTATA2600.022.5312251
TTAGACC500.002262964622.4992923
CGCGCTT2300.022.49929212
GCGCACC602.4648994E-422.49929230
AATACCG500.002262964622.4992925
TTCGGCA1850.021.89120333
GGGGCAA2800.021.69574518
ATACCCG2700.021.6659855
TCTATAA855.0227045E-621.1758042
TGAGTTG2050.020.85300342
Produced by FastQC (version 0.11.2)