FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_11.34100000009734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_11.34100000009734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769785
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10100.13120546646141454No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC9720.1262690231688069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.11717557499821378No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC8670.11262885091291724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATA500.00224727422.5286181
CGCATAC500.002264315522.499353
TTATACG602.4669644E-422.499354
GGGACCG602.4669644E-422.499356
CTAGTAC500.002264315522.499353
GCCTCGA2800.022.4993532
CTAGACA955.5283635E-721.3151744
ACCTATA2750.021.2997841
TAGTACC855.0299022E-621.175864
GCCCACG654.2373047E-420.7686318
CCGAGCG550.00393603320.4539559
GCCAGGC2750.020.45395527
CGTCGGG2800.020.08870514
ATACCGT908.247496E-619.9994246
CGGGGCA3750.019.79942917
GTATACA807.485355E-519.712541
GCCCAAT807.560533E-519.68693211
CGGCCTA1950.019.61481912
ATACCCG3100.019.5962095
CATTATA1151.5941077E-719.5646532
Produced by FastQC (version 0.11.2)