FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_10.34100000009727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_10.34100000009727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774015
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGG551.3679055E-424.544752
GGTATCA11150.024.4479261
GTATCAA23900.023.0940281
CCTACAC855.029955E-621.1758613
GTATTAA654.2005145E-420.7955021
GTATTAC550.00390668320.480421
GGGGCGA550.003936048620.45395918
ATACCAT908.247578E-619.9994266
GCCCACG807.560593E-519.68693418
CCCACGA807.560593E-519.68693419
CTATATA706.921509E-419.3101081
AACGCAG28200.018.9090336
GGCCTAC600.00646128818.7737181
TACACGT600.006509697518.7494625
TTAGTGC851.204061E-418.528883
GCAGCCC851.204061E-418.5288827
CGCAGAG29450.018.2592378
ACGCAGA29700.018.1055417
TATCAAC29800.018.0447832
CGGGCCA750.001109991617.99948517
Produced by FastQC (version 0.11.2)