FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_3_1.34100000009685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_3_1.34100000009685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245979
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAG250.002104363435.998377
GTACTAT300.00508970730.0597531
GTGCAAC300.00514062329.9986449
CGGGGAA300.00514062329.99864417
GGTATCA1400.025.7655011
AAATTCG450.001224136924.9988716
TTAGACA602.46062E-422.4989834
GTATCAA3300.021.1784631
GTGCAGG550.003882851220.4952871
TAGACAG550.003928909520.453625
TCGCTCA550.003928909520.4536222
TGGAGTG807.534798E-519.6866115
TAAGACA706.9642504E-419.2848424
CAGGAAT600.00649798818.7491534
TAGGCCA600.00649798818.7491534
TTTTAGA600.00649798818.7491532
CAACGCA3800.017.1702775
AACACAG800.001706203216.8742395
TCAACGC3900.016.7300134
AACGCAG3950.016.5182426
Produced by FastQC (version 0.11.2)