FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_2_1.3410000000966b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_2_1.3410000000966b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265427
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1750.028.3222371
GTATCAA4700.025.8844411
ACGCAGA5250.022.7136217
CAACGCA5300.022.499345
AACGCAG5300.022.499346
TCAACGC5400.022.0826854
TATCAAC5500.021.6811852
GCAGAGT5800.021.335589
AGAGTAC5750.021.12981611
CTAAGCA654.227244E-420.7686214
ACGGGAC654.227244E-420.76862116
CGCAGAG5750.020.7385238
CAGAGTA5900.020.59261710
GTATTAT550.003899424320.4809551
ATTAAAC550.00392935520.4539453
ACGGGAT550.00392935520.45394516
CTAGCAA550.00392935520.45394522
ATCAACG5900.020.2112733
ACGGGGG908.214134E-619.99941416
TATATAC807.536752E-519.6869223
Produced by FastQC (version 0.11.2)