FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_1_9.3410000000960e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_1_9.3410000000960e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568043
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG11820.2080828387991754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.20192133342018123No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC11390.20051298933355396No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC9120.1605512258755059No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8100.14259483877100854No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7750.13643333339201433No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7430.13079995704550537No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA7190.12657492478562363No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA6900.12146967747159985No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA6740.11865298929834536No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.11178731187603756No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT6190.10897062370278307No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG6120.10773832262698421No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT5920.1042174624104161No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5890.1036893333779309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.10280911832378888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG650.041.5370843
GCCCACG700.038.57014518
CGTATGC803.6379788E-1233.7488841
CCCACGA855.456968E-1231.7636519
GTATGCC855.456968E-1231.7636542
CCCCTGA300.005108379830.0439641
GTGTAAT453.4530705E-530.0439621
CTCGTAT901.2732926E-1129.99900239
ACTGTAT901.2732926E-1129.99900232
GCCCGCC300.005145928429.99900238
GTCTACA554.1129006E-628.678331
AACTGTA952.3646862E-1128.42010931
ATGCCGT952.3646862E-1128.42010944
GTATCTC1004.5474735E-1126.99910435
TCGTATG1004.5474735E-1126.99910440
ACCTATA2900.026.4179671
TGTATCT1200.026.24912634
TATCTCG1159.094947E-1225.43393536
CGTCGGG2950.025.16865314
GGGGCAA3000.024.74917618
Produced by FastQC (version 0.11.2)