FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_1_5.34100000009586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_1_5.34100000009586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614470
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32550.5297248034891857No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA21500.34989503149055284No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG18400.2994450502058685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.2893550539489316No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16280.26494377268214886No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG15970.25989877455368043No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC15300.24899506892118412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.22458378765440137No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAA13340.21709766139925463No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATT11830.19252363825736002No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11670.18991976825556983No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11560.1881296076293391No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9990.16257913323677314No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA9930.16160268198610184No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT9850.16030074698520677No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA9590.15606945823229773No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC8900.14484026884957768No Hit
GCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG8860.14418930134913016No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8410.13686591696909534No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG8020.1305189838397318No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA7900.12856608133838918No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC7870.12807785571305352No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC7790.12677592071215846No Hit
TGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAA7290.11863882695656419No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7000.11391931257831953No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6970.11343108695298387No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA6930.11278011945253634No Hit
ATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACT6890.11212915195208878No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT6820.11098995882630561No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCG6460.10513125132227774No Hit
GCATTGCGATGGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCAGTG6340.1031783488209351No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGG6230.10138818819470437No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG6220.10122544631959249No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATG6220.10122544631959249No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG6220.10122544631959249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT800.039.37350539
GTATGCC800.039.37350542
TCGTATG800.039.37350540
TTATCTC900.037.49857735
CGCACAG301.1395584E-437.4985775
CGTATGC850.037.05741541
ATGCCGT850.037.05741544
TATGCCG900.034.99867243
ACTGTAA458.6937507E-734.99867220
TACTTAT950.033.1566432
TATCTCG950.033.1566436
AGACCTC950.033.1566425
CTTATCT1050.032.14163634
CCCACGA1000.031.49880419
GACCTCT1000.031.49880426
GCCCACG1100.028.63527718
TGTAATT554.1620224E-628.63527722
CACGAGA1200.028.12393421
TAACCGG803.3905962E-928.12393244
GGCGCTA406.164221E-428.12393213
Produced by FastQC (version 0.11.2)