FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_1_4.3410000000956c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_1_4.3410000000956c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946993
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC300.005146865729.99992845
GTATGCC957.421477E-1026.05119542
CGTATGC1001.3460522E-924.74863641
CGCGTTA651.5240335E-524.22943341
GGTATCA11250.024.0545621
ATGCCGT1152.6557245E-1023.47820544
TATTAGG702.7034941E-522.498762
TACCGAC500.00226504322.498767
GTATCAA23650.022.0267051
TATGCCG1257.512426E-1021.5999543
ATAGCAC654.2390486E-420.7680853
GACCTAT1452.1827873E-1020.171326
CGGGGTC908.252664E-619.99889817
ATACAGG706.9848157E-419.284656
CGAGACC1656.002665E-1119.08985723
CTATAAT951.2899218E-518.9904441
GTCGTAC951.2899218E-518.9904441
GTATATA1354.1602107E-818.3750131
GCCCACG1354.2724423E-818.33232318
CCGAGCC1507.2650437E-917.99900814
Produced by FastQC (version 0.11.2)