FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_1_3.34100000009543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_1_3.34100000009543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255641
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG21530.8421966742423946No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19670.7694383921201998No Hit
GTATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCA17560.6869007710030863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17110.6692979608122328No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC14830.5801103891785746No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13750.5378636447205261No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAA13580.5312136942039813No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTC12920.5053962392573962No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGGA11720.45845541208178653No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCT11040.4318556100156078No Hit
GAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGC10820.42324979170007937No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10710.41894688254231516No Hit
TATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCAT10220.39977937811227465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.39899703099268113No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGC9510.37200605536670567No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGC9150.3579238072140228No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGG9110.3563591129748358No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGG8880.34736212109951065No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8740.3418856912623562No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGT8650.3383651292241855No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTC8140.3184152776745514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.31763293055495795No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATAT7880.308244765119836No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.2972919054455271No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGA7400.28946843424959223No Hit
GGTATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC7380.2886860871299987No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACG6660.260521590824633No Hit
CATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGC6230.24370112775337288No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTT5870.22961887960069005No Hit
GTATCAACGCAGAGTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGG5790.22648949112231606No Hit
GCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAG5620.21983954060577135No Hit
CCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGA5230.20458377177369827No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5200.20341025109430802No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.18658978802304796No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4700.18385157310447073No Hit
GTATCAACGCAGAGTACGGGCAGGCCTCGATGAGTAGGAGGGCGCGGCGGT4670.1826780524250805No Hit
CCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACC4620.18072218462609674No Hit
ACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCC4600.17993983750650328No Hit
GAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACT4490.17563692834873906No Hit
TCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCC4410.17250753987036507No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4380.17133401919097482No Hit
GTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCG4260.1666399364734139No Hit
TGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCT4190.16390172155483668No Hit
ATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGT4130.16155468019605618No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.15959881239707246No Hit
GTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGC4020.157251771038292No Hit
TCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGG3850.1506018205217473No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA3820.14942829984235706No Hit
GACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGC3800.14864595272276354No Hit
GGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGA3750.14669008492377983No Hit
AATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACT3580.14004013440723515No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.1388666137278449No Hit
ACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTG3480.1361283988092677No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTG3440.1345637045700807No Hit
GTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACC3410.13339018389069046No Hit
CCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAA3400.13299901033089373No Hit
ACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT3230.12634905981434905No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA3220.1259578862545523No Hit
TATCAACGCAGAGTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGT3200.1251755391349588No Hit
CTCATAGGCAGTGGCTTGGTTAAGGGAACCCACCGGAGCCGTAGCGAAAGC3180.12439319201536529No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG3120.12204615065658482No Hit
CTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCC3090.12087262997719458No Hit
CTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGAT3080.12048145641739784No Hit
GAGTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAA3030.11852558861841411No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3000.11735206793902386No Hit
AACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG2990.11696089437922713No Hit
GCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG2940.11500502658024338No Hit
ATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATG2880.1126579852214629No Hit
GTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATA2790.1091374231832922No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATT2770.1083550760636987No Hit
GGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGG2740.10718155538430847No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2730.10679038182451171No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAAC2710.10600803470491822No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA2690.10522568758532472No Hit
ATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAA2670.10444334046573124No Hit
GCCCGGGCTCGCGCCCTAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCA2660.1040521669059345No Hit
TATCAACGCAGAGTACGGGCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTC2640.103269819786341No Hit
TTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCC2620.10248747266674751No Hit
CCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTT2620.10248747266674751No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTTA207.0240366E-444.9986331
CTCGTAT250.002104365335.99890539
CTTATCT250.002104365335.99890534
TATCTCG250.002104365335.99890536
GTCTTAT250.002104365335.99890532
AGTGTCT250.002104365335.99890529
ACAGTGT300.005140625429.99908627
TATGCCG300.005140625429.99908643
GTGTCTT300.005140625429.99908630
TCGTATG300.005140625429.99908640
CACACAG300.005140625429.9990865
ATGCCGT300.005140625429.99908644
ACGAGAC300.005140625429.99908622
TGCCGTC300.005140625429.99908645
GCCCACG406.154021E-428.12414418
TAAAGAG406.154021E-428.1241445
GCGCGCT406.154021E-428.12414411
CTGGCTC406.154021E-428.12414418
CCGGCCG755.604852E-826.9991789
GGATAGC507.1384886E-526.99917835
Produced by FastQC (version 0.11.2)