FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb3_1_10.34100000009628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb3_1_10.34100000009628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344000
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.5738372093023256No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19240.5593023255813954No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14220.41337209302325584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.3168604651162791No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10520.3058139534883721No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10000.29069767441860467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.24738372093023256No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8110.23575581395348838No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6510.1892441860465116No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6230.18110465116279068No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG4840.14069767441860465No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.13517441860465115No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC4480.13023255813953488No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4350.12645348837209303No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.1183139534883721No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG4050.11773255813953488No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4030.11715116279069768No Hit
GTATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCA3750.10901162790697674No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC3670.1066860465116279No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3640.1058139534883721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAG250.002090414336.0512351
GCTAACA300.005143117629.9990341
TAATATT406.1584543E-428.1240924
TATGCCG507.145642E-526.99912843
GTATGCC507.145642E-526.99912842
ACTGATT450.001225038424.99919327
TGATTAC450.001225038424.99919329
AGATCGG1001.3369572E-924.749210
GGTTCAA952.1052983E-823.68344515
AATGCAA1052.3537723E-923.57066727
ACGAATG1152.6375346E-1023.477545
TGCAAAG1156.730261E-921.52104229
GACCCGG855.0167146E-621.17578717
ATTCACG855.0167146E-621.17578724
CTACACA1501.4551915E-1120.9993214
GGTGGGA1401.364242E-1020.89218111
ACCTGCA1301.2187229E-920.76855939
TTAATAT550.003931753320.4538863
TTAGATA550.003931753320.4538864
ATTACAA550.003931753320.45388631
Produced by FastQC (version 0.11.2)