##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23V3BCXX l01n01 kb2_4_94.3410000000b2fc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.70454545454545	0.0	0.0	0.0	0.0	0.0
2	36.0	0.0	0.0	0.0	0.0	0.0
3	35.36363636363637	0.0	0.0	0.0	0.0	0.0
4	35.54545454545455	0.0	0.0	0.0	0.0	0.0
5	35.84090909090909	0.0	0.0	0.0	0.0	0.0
6	36.93181818181818	0.0	0.0	0.0	0.0	0.0
7	37.27272727272727	0.0	0.0	0.0	0.0	0.0
8	36.95454545454545	0.0	0.0	0.0	0.0	0.0
9	37.45454545454545	0.0	0.0	0.0	0.0	0.0
10	36.43181818181818	0.0	0.0	0.0	0.0	0.0
11	37.81818181818182	0.0	0.0	0.0	0.0	0.0
12	36.65909090909091	0.0	0.0	0.0	0.0	0.0
13	37.34090909090909	0.0	0.0	0.0	0.0	0.0
14	37.02272727272727	0.0	0.0	0.0	0.0	0.0
15	37.38636363636363	0.0	0.0	0.0	0.0	0.0
16	37.27272727272727	0.0	0.0	0.0	0.0	0.0
17	38.04545454545455	0.0	0.0	0.0	0.0	0.0
18	37.0	0.0	0.0	0.0	0.0	0.0
19	36.88636363636363	0.0	0.0	0.0	0.0	0.0
20	36.63636363636363	0.0	0.0	0.0	0.0	0.0
21	38.20454545454545	0.0	0.0	0.0	0.0	0.0
22	37.61363636363637	0.0	0.0	0.0	0.0	0.0
23	37.36363636363637	0.0	0.0	0.0	0.0	0.0
24	37.70454545454545	0.0	0.0	0.0	0.0	0.0
25	37.34090909090909	0.0	0.0	0.0	0.0	0.0
26	36.54545454545455	0.0	0.0	0.0	0.0	0.0
27	35.95454545454545	0.0	0.0	0.0	0.0	0.0
28	37.79545454545455	0.0	0.0	0.0	0.0	0.0
29	37.5	0.0	0.0	0.0	0.0	0.0
30	36.22727272727273	0.0	0.0	0.0	0.0	0.0
31	36.5	0.0	0.0	0.0	0.0	0.0
32	37.45454545454545	0.0	0.0	0.0	0.0	0.0
33	36.09090909090909	0.0	0.0	0.0	0.0	0.0
34	36.54545454545455	0.0	0.0	0.0	0.0	0.0
35	35.47727272727273	0.0	0.0	0.0	0.0	0.0
36	37.5	0.0	0.0	0.0	0.0	0.0
37	35.95454545454545	0.0	0.0	0.0	0.0	0.0
38	37.04545454545455	0.0	0.0	0.0	0.0	0.0
39	37.11363636363637	0.0	0.0	0.0	0.0	0.0
40	36.36363636363637	0.0	0.0	0.0	0.0	0.0
41	36.70454545454545	0.0	0.0	0.0	0.0	0.0
42	37.0	0.0	0.0	0.0	0.0	0.0
43	36.63636363636363	0.0	0.0	0.0	0.0	0.0
44	34.93181818181818	0.0	0.0	0.0	0.0	0.0
45	36.36363636363637	0.0	0.0	0.0	0.0	0.0
46	35.0	0.0	0.0	0.0	0.0	0.0
47	36.45454545454545	0.0	0.0	0.0	0.0	0.0
48	35.20454545454545	0.0	0.0	0.0	0.0	0.0
49	35.65909090909091	0.0	0.0	0.0	0.0	0.0
50	36.22727272727273	0.0	0.0	0.0	0.0	0.0
51	35.43181818181818	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1202	1	3.0
1202	2	3.0
1202	3	-3.0
1202	4	-8.25
1202	5	-6.75
1202	6	-4.0
1202	7	2.5
1202	8	-7.25
1202	9	1.0
1202	10	5.25
1202	11	-7.25
1202	12	2.75
1202	13	-5.75
1202	14	2.25
1202	15	-4.5
1202	16	1.0
1202	17	-0.5
1202	18	-0.5
1202	19	5.75
1202	20	-3.5
1202	21	-0.5
1202	22	-1.0
1202	23	1.0
1202	24	-5.5
1202	25	1.0
1202	26	-0.5
1202	27	-17.25
1202	28	1.5
1202	29	1.5
1202	30	3.75
1202	31	-2.25
1202	32	2.25
1202	33	-0.5
1202	34	1.0
1202	35	5.75
1202	36	-5.0
1202	37	-2.0
1202	38	-8.75
1202	39	-7.25
1202	40	1.0
1202	41	2.25
1202	42	7.25
1202	43	2.5
1202	44	-17.75
1202	45	-7.25
1202	46	-1.75
1202	47	0.0
1202	48	0.5
1202	49	-5.0
1202	50	-8.75
1202	51	1.0
1216	1	-3.0
1216	2	3.0
1216	3	3.0
1216	4	2.75
1216	5	4.25
1216	6	4.0
1216	7	4.5
1216	8	5.75
1216	9	3.0
1216	10	7.25
1216	11	5.75
1216	12	10.75
1216	13	7.25
1216	14	4.25
1216	15	1.5
1216	16	3.0
1216	17	1.5
1216	18	1.5
1216	19	7.75
1216	20	4.5
1216	21	1.5
1216	22	1.0
1216	23	1.0
1216	24	2.5
1216	25	3.0
1216	26	1.5
1216	27	7.75
1216	28	1.5
1216	29	1.5
1216	30	5.75
1216	31	5.75
1216	32	4.25
1216	33	1.5
1216	34	3.0
1216	35	7.75
1216	36	3.0
1216	37	9.0
1216	38	4.25
1216	39	5.75
1216	40	3.0
1216	41	4.25
1216	42	9.25
1216	43	4.5
1216	44	9.25
1216	45	5.75
1216	46	4.25
1216	47	0.0
1216	48	2.5
1216	49	3.0
1216	50	4.25
1216	51	3.0
2114	1	-3.0
2114	2	-3.0
2114	3	3.0
2114	4	2.75
2114	5	-1.75
2114	6	-4.0
2114	7	-3.5
2114	8	-2.25
2114	9	-5.0
2114	10	-19.75
2114	11	-2.25
2114	12	-16.25
2114	13	-0.75
2114	14	-8.75
2114	15	1.5
2114	16	-5.0
2114	17	-0.5
2114	18	-0.5
2114	19	-19.25
2114	20	-3.5
2114	21	-0.5
2114	22	-1.0
2114	23	-1.0
2114	24	0.5
2114	25	-5.0
2114	26	-0.5
2114	27	7.75
2114	28	-4.5
2114	29	-4.5
2114	30	-7.25
2114	31	-7.25
2114	32	-8.75
2114	33	-0.5
2114	34	-5.0
2114	35	-19.25
2114	36	1.0
2114	37	-16.0
2114	38	2.25
2114	39	-2.25
2114	40	-5.0
2114	41	-8.75
2114	42	-17.75
2114	43	-3.5
2114	44	1.25
2114	45	-2.25
2114	46	-6.75
2114	47	0.0
2114	48	-5.5
2114	49	1.0
2114	50	2.25
2114	51	-5.0
2212	1	3.0
2212	2	-3.0
2212	3	-3.0
2212	4	2.75
2212	5	4.25
2212	6	4.0
2212	7	-3.5
2212	8	3.75
2212	9	1.0
2212	10	7.25
2212	11	3.75
2212	12	2.75
2212	13	-0.75
2212	14	2.25
2212	15	1.5
2212	16	1.0
2212	17	-0.5
2212	18	-0.5
2212	19	5.75
2212	20	2.5
2212	21	-0.5
2212	22	1.0
2212	23	-1.0
2212	24	2.5
2212	25	1.0
2212	26	-0.5
2212	27	1.75
2212	28	1.5
2212	29	1.5
2212	30	-2.25
2212	31	3.75
2212	32	2.25
2212	33	-0.5
2212	34	1.0
2212	35	5.75
2212	36	1.0
2212	37	9.0
2212	38	2.25
2212	39	3.75
2212	40	1.0
2212	41	2.25
2212	42	1.25
2212	43	-3.5
2212	44	7.25
2212	45	3.75
2212	46	4.25
2212	47	0.0
2212	48	2.5
2212	49	1.0
2212	50	2.25
2212	51	1.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	0.0
29	3.0
30	0.0
31	1.0
32	0.0
33	4.0
34	1.0
35	2.0
36	2.0
37	6.0
38	5.0
39	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.090909090909086	34.090909090909086	11.363636363636363	20.454545454545457
2	11.363636363636363	18.181818181818183	65.9090909090909	4.545454545454546
3	15.909090909090908	18.181818181818183	25.0	40.909090909090914
4	13.636363636363635	20.454545454545457	38.63636363636363	27.27272727272727
5	22.727272727272727	20.454545454545457	45.45454545454545	11.363636363636363
6	34.090909090909086	29.545454545454547	15.909090909090908	20.454545454545457
7	29.545454545454547	25.0	15.909090909090908	29.545454545454547
8	31.818181818181817	31.818181818181817	20.454545454545457	15.909090909090908
9	20.454545454545457	18.181818181818183	15.909090909090908	45.45454545454545
10	11.363636363636363	31.818181818181817	29.545454545454547	27.27272727272727
11	34.090909090909086	34.090909090909086	15.909090909090908	15.909090909090908
12	20.454545454545457	15.909090909090908	36.36363636363637	27.27272727272727
13	20.454545454545457	27.27272727272727	27.27272727272727	25.0
14	22.727272727272727	9.090909090909092	25.0	43.18181818181818
15	22.727272727272727	36.36363636363637	13.636363636363635	27.27272727272727
16	20.454545454545457	40.909090909090914	20.454545454545457	18.181818181818183
17	13.636363636363635	27.27272727272727	22.727272727272727	36.36363636363637
18	31.818181818181817	31.818181818181817	18.181818181818183	18.181818181818183
19	20.454545454545457	38.63636363636363	25.0	15.909090909090908
20	18.181818181818183	27.27272727272727	29.545454545454547	25.0
21	20.454545454545457	22.727272727272727	18.181818181818183	38.63636363636363
22	40.909090909090914	20.454545454545457	31.818181818181817	6.8181818181818175
23	20.454545454545457	36.36363636363637	25.0	18.181818181818183
24	22.727272727272727	34.090909090909086	18.181818181818183	25.0
25	25.0	27.27272727272727	13.636363636363635	34.090909090909086
26	15.909090909090908	40.909090909090914	27.27272727272727	15.909090909090908
27	18.181818181818183	22.727272727272727	22.727272727272727	36.36363636363637
28	25.0	22.727272727272727	18.181818181818183	34.090909090909086
29	22.727272727272727	31.818181818181817	11.363636363636363	34.090909090909086
30	22.727272727272727	27.27272727272727	31.818181818181817	18.181818181818183
31	18.181818181818183	20.454545454545457	34.090909090909086	27.27272727272727
32	18.181818181818183	40.909090909090914	25.0	15.909090909090908
33	27.27272727272727	18.181818181818183	34.090909090909086	20.454545454545457
34	36.36363636363637	22.727272727272727	22.727272727272727	18.181818181818183
35	20.454545454545457	27.27272727272727	27.27272727272727	25.0
36	27.27272727272727	25.0	31.818181818181817	15.909090909090908
37	25.0	27.27272727272727	15.909090909090908	31.818181818181817
38	20.454545454545457	29.545454545454547	36.36363636363637	13.636363636363635
39	27.27272727272727	20.454545454545457	27.27272727272727	25.0
40	27.27272727272727	29.545454545454547	27.27272727272727	15.909090909090908
41	15.909090909090908	27.27272727272727	20.454545454545457	36.36363636363637
42	18.181818181818183	34.090909090909086	22.727272727272727	25.0
43	20.454545454545457	34.090909090909086	31.818181818181817	13.636363636363635
44	25.0	38.63636363636363	18.181818181818183	18.181818181818183
45	27.27272727272727	25.0	29.545454545454547	18.181818181818183
46	27.27272727272727	20.454545454545457	31.818181818181817	20.454545454545457
47	22.727272727272727	25.0	34.090909090909086	18.181818181818183
48	20.454545454545457	25.0	27.27272727272727	27.27272727272727
49	25.0	25.0	22.727272727272727	27.27272727272727
50	11.363636363636363	22.727272727272727	36.36363636363637	29.545454545454547
51	18.181818181818183	9.090909090909092	43.18181818181818	29.545454545454547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	0.5
27	0.0
28	1.0
29	2.0
30	1.5
31	1.0
32	1.0
33	1.0
34	2.5
35	4.0
36	4.0
37	4.0
38	3.0
39	2.0
40	3.0
41	4.0
42	3.5
43	3.0
44	3.0
45	3.0
46	2.5
47	2.0
48	2.0
49	2.0
50	1.5
51	1.0
52	2.5
53	4.0
54	2.5
55	1.0
56	0.5
57	0.0
58	0.0
59	0.0
60	1.5
61	3.0
62	1.5
63	0.0
64	0.5
65	1.0
66	1.5
67	2.0
68	1.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.5
75	1.5
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	44.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT	1	2.272727272727273	RNA PCR Primer, Index 37 (95% over 22bp)
GTTATTCACAACCGAAGAATCTACTGATCTCAATCTCTTCACACTGTCTCT	1	2.272727272727273	No Hit
TTCAAGTACTTACTTAAGTGCATTGAGAACCATCCTAAAGATGACACCTCT	1	2.272727272727273	No Hit
GTCGTCCTTGGTCTTGAGCCGTGCGAGGCTCACGAAGGCCGACGGGTTGCG	1	2.272727272727273	No Hit
ATGCTGAGGTATTCACCATTGGTTCTTCATCAGGTTCTGATATCTGTCTCT	1	2.272727272727273	No Hit
TATTCAGGCTGTCTCTGAAATGTAATCACAATCTGTCTCTTATACACATCT	1	2.272727272727273	No Hit
GTTTAGAACCGATCGATACTGTAACAGCAGCTTGCAGCTTCTGGTTTAACC	1	2.272727272727273	No Hit
GTACGGGGTGGTACCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTCTC	1	2.272727272727273	No Hit
ATTAGTCACCACTCCTCTTTCGATCATCACACCATTATGATCATTTGTTTG	1	2.272727272727273	No Hit
AGCCTGAGCATCGGGCTGTGCGGCCTCGCTCAGGCGCTCGCGCTGCAGGGC	1	2.272727272727273	No Hit
AACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTG	1	2.272727272727273	No Hit
GTCTGCATACCATGGTTGAAGGAAAGGGCTTCCTCGTGAGCTCAGTGACTG	1	2.272727272727273	No Hit
ATCCTCCTGGCTTATTCTTTTCAATCTTGCTTTTGTCAATCTCATTAGCCT	1	2.272727272727273	No Hit
GGATGCCACCACCCCCAAATACTCCAAGGCTAGGTACGATGAAATCATCAA	1	2.272727272727273	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	1	2.272727272727273	RNA PCR Primer, Index 23 (95% over 21bp)
GTCTTATTTTGTTCAAAATCACTAAAGGCTTTACTGTGTTCAATATTATTA	1	2.272727272727273	No Hit
TTGTAACCCTGTGCAATTGTCTGCCAATTTCCTCAGCCTTTCAAGGCAGGT	1	2.272727272727273	No Hit
CGACGGCGCGCTGGAGCTGGCGCGCACCCAGCCCAACGTGCTGGTGACGCG	1	2.272727272727273	No Hit
ACCGTGGCGAGCGAAAGCGCCTGCTTCTCGTTGTAGGAGTAGCCGGTGAAC	1	2.272727272727273	No Hit
CAAGAGAAGAAGAGCAAGAAGAAAGATAAGAAAGCGAAGAAAGAGAAGAAC	1	2.272727272727273	No Hit
GTCGAAGGCTCTGTCTCGACCGAAAATGCTGTTGTTGAGTCCAAGAATGCC	1	2.272727272727273	No Hit
GTCCTTTAACATTCCTCATGATGTAACGATCAGAGTCGGTAAACTTCACTC	1	2.272727272727273	No Hit
ATATCGAAGAAACGAAATTTATAAGCAACGGCTCCATTGAAAATAAGGAAA	1	2.272727272727273	No Hit
CTTCAGAGAAGGAGAACCAAATGCTTTCATTTCGCGGATCATGGACAAGAT	1	2.272727272727273	No Hit
TTCTCCCATTAGACGACGATCGTACGGCGAAGAAAGAAGGAGAAGATCTTT	1	2.272727272727273	No Hit
CATATGTAGCAGAGGATTGCCTTGTAAGACATCAATGGGAGAAGAGGCCCT	1	2.272727272727273	No Hit
CATCTGGGCTGTACACCGCGTGGGCCACCGCGGCACGTTGCGTGAATTCGT	1	2.272727272727273	No Hit
ATCACGAACCAGTCGCTGTTCTCCAGCGCGGCGATCGAGCCTTCCGACTTG	1	2.272727272727273	No Hit
ATCATCCAATTCGCTCCAAGAGAGAGGCATGGTTTTGTAGTCAAAGCAACT	1	2.272727272727273	No Hit
CTCTTTCTGATGCTCATCAACGTGGACCGCTATGCGGCCATCGTGCTGTCT	1	2.272727272727273	No Hit
ACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACT	1	2.272727272727273	No Hit
GTCCTTCTTCAGCCACTGAAGAAAATTCTATGTGGATGCAGGTACTGAGTA	1	2.272727272727273	No Hit
AGCTCGGTGAACGCAAAGGCCTTCCACATGTTCTTAGTCAAAACTGAGAAT	1	2.272727272727273	No Hit
GTGTGGGGCCGCAACGTCACCAACGTCGCTTATGCCACCAATTACTTCAAT	1	2.272727272727273	No Hit
GTGTGTATACAGATAACGAAGGTATTCCAATGAGAAACATGTTAACACATT	1	2.272727272727273	No Hit
AATGTACGTAGAATACCAAAGGGAGTTATTCATGTTCAAGCAAGTTTCAAC	1	2.272727272727273	No Hit
GAAGGCGCCAGTCCGACCGCCGGACCCGAGCGGGTCAAGGCCGGGCGTCTC	1	2.272727272727273	No Hit
CTATTTTAAGATACATTGCGAGATAACTAAATTAACAAAATCTCCTGTCTC	1	2.272727272727273	No Hit
ATTATATGAAGGAGGGAAAAAACGCCATGCGATATTAATCTCTATTTGCTG	1	2.272727272727273	No Hit
ATGTAATGGAGCTTTTGATGTAAGGACACATAAAGACTTAGAAGAGGAAAG	1	2.272727272727273	No Hit
TGGCAAACCAAATCTACTTATTCTCCTTGATCTGCTTGTCCTGTCTCTTAT	1	2.272727272727273	No Hit
CTCTTAGCCTCACGGGGAATATCTCTGAGGCGTAGACCCACGTCACGGGGC	1	2.272727272727273	No Hit
GATTGAGCTCGTGCATATATTGCAGACAATAAGCAGTTCCCATTATCTGTC	1	2.272727272727273	No Hit
GTCTTCGGATTTCTCGCAGAGTTTCTCCGCTAGGGCTTTGCCTTTCTCGGT	1	2.272727272727273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
25	0.0	0.0	0.0
26	0.0	0.0	0.0
27	0.0	0.0	0.0
28	0.0	0.0	0.0
29	0.0	0.0	0.0
30	0.0	0.0	0.0
31	0.0	0.0	0.0
32	0.0	0.0	0.0
33	0.0	0.0	2.272727272727273
34	0.0	0.0	2.272727272727273
35	0.0	0.0	2.272727272727273
36	0.0	0.0	2.272727272727273
37	0.0	0.0	2.272727272727273
38	0.0	0.0	2.272727272727273
39	0.0	0.0	2.272727272727273
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE