FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_94.3410000000b2fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_94.3410000000b2fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT12.272727272727273RNA PCR Primer, Index 37 (95% over 22bp)
GTTATTCACAACCGAAGAATCTACTGATCTCAATCTCTTCACACTGTCTCT12.272727272727273No Hit
TTCAAGTACTTACTTAAGTGCATTGAGAACCATCCTAAAGATGACACCTCT12.272727272727273No Hit
GTCGTCCTTGGTCTTGAGCCGTGCGAGGCTCACGAAGGCCGACGGGTTGCG12.272727272727273No Hit
ATGCTGAGGTATTCACCATTGGTTCTTCATCAGGTTCTGATATCTGTCTCT12.272727272727273No Hit
TATTCAGGCTGTCTCTGAAATGTAATCACAATCTGTCTCTTATACACATCT12.272727272727273No Hit
GTTTAGAACCGATCGATACTGTAACAGCAGCTTGCAGCTTCTGGTTTAACC12.272727272727273No Hit
GTACGGGGTGGTACCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTCTC12.272727272727273No Hit
ATTAGTCACCACTCCTCTTTCGATCATCACACCATTATGATCATTTGTTTG12.272727272727273No Hit
AGCCTGAGCATCGGGCTGTGCGGCCTCGCTCAGGCGCTCGCGCTGCAGGGC12.272727272727273No Hit
AACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTG12.272727272727273No Hit
GTCTGCATACCATGGTTGAAGGAAAGGGCTTCCTCGTGAGCTCAGTGACTG12.272727272727273No Hit
ATCCTCCTGGCTTATTCTTTTCAATCTTGCTTTTGTCAATCTCATTAGCCT12.272727272727273No Hit
GGATGCCACCACCCCCAAATACTCCAAGGCTAGGTACGATGAAATCATCAA12.272727272727273No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC12.272727272727273RNA PCR Primer, Index 23 (95% over 21bp)
GTCTTATTTTGTTCAAAATCACTAAAGGCTTTACTGTGTTCAATATTATTA12.272727272727273No Hit
TTGTAACCCTGTGCAATTGTCTGCCAATTTCCTCAGCCTTTCAAGGCAGGT12.272727272727273No Hit
CGACGGCGCGCTGGAGCTGGCGCGCACCCAGCCCAACGTGCTGGTGACGCG12.272727272727273No Hit
ACCGTGGCGAGCGAAAGCGCCTGCTTCTCGTTGTAGGAGTAGCCGGTGAAC12.272727272727273No Hit
CAAGAGAAGAAGAGCAAGAAGAAAGATAAGAAAGCGAAGAAAGAGAAGAAC12.272727272727273No Hit
GTCGAAGGCTCTGTCTCGACCGAAAATGCTGTTGTTGAGTCCAAGAATGCC12.272727272727273No Hit
GTCCTTTAACATTCCTCATGATGTAACGATCAGAGTCGGTAAACTTCACTC12.272727272727273No Hit
ATATCGAAGAAACGAAATTTATAAGCAACGGCTCCATTGAAAATAAGGAAA12.272727272727273No Hit
CTTCAGAGAAGGAGAACCAAATGCTTTCATTTCGCGGATCATGGACAAGAT12.272727272727273No Hit
TTCTCCCATTAGACGACGATCGTACGGCGAAGAAAGAAGGAGAAGATCTTT12.272727272727273No Hit
CATATGTAGCAGAGGATTGCCTTGTAAGACATCAATGGGAGAAGAGGCCCT12.272727272727273No Hit
CATCTGGGCTGTACACCGCGTGGGCCACCGCGGCACGTTGCGTGAATTCGT12.272727272727273No Hit
ATCACGAACCAGTCGCTGTTCTCCAGCGCGGCGATCGAGCCTTCCGACTTG12.272727272727273No Hit
ATCATCCAATTCGCTCCAAGAGAGAGGCATGGTTTTGTAGTCAAAGCAACT12.272727272727273No Hit
CTCTTTCTGATGCTCATCAACGTGGACCGCTATGCGGCCATCGTGCTGTCT12.272727272727273No Hit
ACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACT12.272727272727273No Hit
GTCCTTCTTCAGCCACTGAAGAAAATTCTATGTGGATGCAGGTACTGAGTA12.272727272727273No Hit
AGCTCGGTGAACGCAAAGGCCTTCCACATGTTCTTAGTCAAAACTGAGAAT12.272727272727273No Hit
GTGTGGGGCCGCAACGTCACCAACGTCGCTTATGCCACCAATTACTTCAAT12.272727272727273No Hit
GTGTGTATACAGATAACGAAGGTATTCCAATGAGAAACATGTTAACACATT12.272727272727273No Hit
AATGTACGTAGAATACCAAAGGGAGTTATTCATGTTCAAGCAAGTTTCAAC12.272727272727273No Hit
GAAGGCGCCAGTCCGACCGCCGGACCCGAGCGGGTCAAGGCCGGGCGTCTC12.272727272727273No Hit
CTATTTTAAGATACATTGCGAGATAACTAAATTAACAAAATCTCCTGTCTC12.272727272727273No Hit
ATTATATGAAGGAGGGAAAAAACGCCATGCGATATTAATCTCTATTTGCTG12.272727272727273No Hit
ATGTAATGGAGCTTTTGATGTAAGGACACATAAAGACTTAGAAGAGGAAAG12.272727272727273No Hit
TGGCAAACCAAATCTACTTATTCTCCTTGATCTGCTTGTCCTGTCTCTTAT12.272727272727273No Hit
CTCTTAGCCTCACGGGGAATATCTCTGAGGCGTAGACCCACGTCACGGGGC12.272727272727273No Hit
GATTGAGCTCGTGCATATATTGCAGACAATAAGCAGTTCCCATTATCTGTC12.272727272727273No Hit
GTCTTCGGATTTCTCGCAGAGTTTCTCCGCTAGGGCTTTGCCTTTCTCGGT12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers