FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_93.3410000000b2ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_93.3410000000b2ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCACCTTGTGGGGTTGGTGCTTCCCACATTTCTTGCAGAATGTCCGGC11.4285714285714286No Hit
GTTACATGCTATACTACATCTGCCCAATGCACACTCTGTTTACTCTAATGG11.4285714285714286No Hit
GGACCAGATCATAAGTGGAGATATATGTGGAGAGTTGGTCCTAGGCCATCT11.4285714285714286No Hit
CTCATGACAAACTCATAGAACTCTTCGAAAACTGGATCTCAAATTTTGAGA11.4285714285714286No Hit
GTATCAACGCAGAGTACGGGATAACAGAAAGAAGAAACACAAAGTGAGACA11.4285714285714286No Hit
AGGGAAGACACCCTTCCGTCGTTTATTCTCAAGATTTTGTACCCCCTGTCT11.4285714285714286No Hit
GCGCGACATCGAACGCGTGCTGTACTTCGAAGCGTTCGTGGTGCTGGAACC11.4285714285714286No Hit
GTATTATTGCACATAGTTGTTACAAGACTTCCGTTGATAAATCCTGTCTCT11.4285714285714286No Hit
GGATAATATCAATCCAGAATCGGTTTAAGGTTAGAGACTGTCTCTTATACA11.4285714285714286No Hit
GTGTAATATGTTTGATTCGTTGTTTTCATGTTTTTGTTTCGGTGGTAATAA11.4285714285714286No Hit
CTAAATACATTTTCTTGAACTCCTCATGGCTCAAATCCGCAAACTCTGTCT11.4285714285714286No Hit
GAAGGGTGGTCAACAAACTACTTCCAAATCTCAGGTGGCAATATTTGTTGC11.4285714285714286No Hit
GTATCAACGCAGAGTACGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGA11.4285714285714286No Hit
CACCTGATGAATGCATCGCGCGAGATCTCCTCGGGCAACCAGGATCTCTCG11.4285714285714286No Hit
TTCCTAAAGGGCGCTGTGAACTGTAATTGTGAAAAGGTTGGAAGATCTGGC11.4285714285714286No Hit
TCTCGATACCGTGCGCCACGCTGTTGCGCAGCAGGTGCTCGAAGGCACCGG11.4285714285714286No Hit
GTTCACGCTCACCATATTTATTTTCATGCTTGCTCTTGTTGGAGTGTTCTA11.4285714285714286No Hit
CCCTACAAGGAACTGAATCCTCAAAAATCTTGTCCATGTTCATATCCATCA11.4285714285714286No Hit
TTCTGGATGTCTCCATCTCAAATTAGCTGTCGAAAACAGCTCTGGGTGTCT11.4285714285714286No Hit
ACCCTCACACCCGCTGGGCAGGCCCATGCGCCGCAGCGACGCACCTGTCTC11.4285714285714286No Hit
GGTCTGAACGGCACGCTGGTCACCGAGTACAAGTTCTCGACCGACCCGCGC11.4285714285714286No Hit
GTCCTGCATGACGGCAACAGTTGTGACTCTAGTTCCAGGGGACCCAAACAC11.4285714285714286No Hit
AAGATGGACTTCAATTCGTGGTTTTCCTACACGTTGGTGTTTCTGTCTCTT11.4285714285714286No Hit
ATGCAACGTTATATGGTTACAGGTACCACCAGAATAACATATAAAAGGCGT11.4285714285714286No Hit
GGTTGGGCTGCCGGAGGACGCCTTCGTCTTCTGCGCGTTCAACAACAACCA11.4285714285714286No Hit
GGGGTTTCTTCCTCTTCAATCGGTAACCTATCATAAACCTGTCTCTTATAC11.4285714285714286No Hit
CCATTCCAAGGAGATTAACAATTCCATCGGTCGAAGAAGATACATGGTCAA11.4285714285714286No Hit
ATACATCATTGAAGGTCTTTCTTCCGGATTCATGTTCGTACCTGTCTCTTA11.4285714285714286No Hit
GAGTATATGTGGCAAATATTCGTCTTAAGGGAGTTGATACAGATGTCTTAG11.4285714285714286No Hit
GATCAACTCAACGCTATTAATTGTCTCTTGATTTATCACTGTCTCTTATAC11.4285714285714286No Hit
GTTGAGTTAAACACCAACGTGTAGGAAAACCACGAATTGAAGTCCATCTTC11.4285714285714286No Hit
CGGTTAAGCTCGCTACTAAACGTAAGTCGCTGACCCATTATGCAAAAGGTA11.4285714285714286No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCTGTCTCTTATAC11.4285714285714286No Hit
ATGATGATCAAGTCGAGATATCTCAAGTCTATCATTGCGATGGCTTATTGT11.4285714285714286No Hit
GCCATTAAAGAAGCAACTTCAGCTTCTATTGATGCAACAGACTGATGAAGC11.4285714285714286No Hit
GTACAAGACTCTTGTGATGCGGGGTGATTTAGACAAAGCCAGTGAAATCTT11.4285714285714286No Hit
CCTTGAAAGAACATAAGCCAATGGCAATCATGATAATGGCTAACTTGATAA11.4285714285714286No Hit
GCAGTAAACATCTTCAAAAGATGGCTTAGTAGAGTCCTATTAACCTGTCTC11.4285714285714286No Hit
CACCCATGCGAATGCTGTCCAAGTTTGTTGTCGCCGGCGCCCTGAGCGCCA11.4285714285714286No Hit
ATACACACATCCCTATGGAGAAGGAAAAAAATGAAAAGGGACAACATAGAA11.4285714285714286No Hit
GTGTAATCGTGGACGACCCGGCCGTCGGGCAGCCGGCCGGCCTCGCGGCGT11.4285714285714286No Hit
GTATCAACGCAGAGTACGGGGGAAGCTATCACTTTAAGAAGAGTAGAAGAC11.4285714285714286No Hit
CCCTTTAAAGAAGCCGAATGCACAGGTGTTAAAGGTTTTAAAATAAACAAG11.4285714285714286No Hit
GGGCATATATAGGTAAACTTCCAGCCTCGTTATGTAGTATAAGGCCTGTCT11.4285714285714286No Hit
TGGATCGGGAGTGCTCAGAGATGGGTGTGCCTCTTTTGGGGTCGGTTCACA11.4285714285714286No Hit
GTCCTGTTTCGTTTTGCTTCTTTGATTCAGGCAATTCCTTGAGGGATGTGG11.4285714285714286No Hit
GAATGAGAATCTAAACCACAAGACTGCTCAAAGGTACTTCCTTTACTTCTG11.4285714285714286No Hit
CAACAGAACCCTAGATTCCGCGTTCTAGCTCCGCACTACGTAATTCATCTC11.4285714285714286No Hit
GATGATGATGATCACACTTCATGAAGCTTGAAGTTTTACTTCTGTCTCTTA11.4285714285714286No Hit
CATGTCTCGTATGACGTCATCTTATTTGTCTCGAATTGAAACTTCTCAATT11.4285714285714286No Hit
CCTTTAACTTCAGACACTTGAGCTTTAACCTTAGCAAGCTTGCTAATCTCA11.4285714285714286No Hit
ATTTACATCTGCTTCTTTCTTTCTTTTTTCCCCTCAAACCATGAGCCTTTG11.4285714285714286No Hit
ATTACCAGTTGATTGATATCACTGAGGATGGCTTCGTGAGCCTTCTCACTG11.4285714285714286No Hit
GGATGTACATGTTCGACATAACGCGAGCCATCCTGCAGCGCTGGAGAATGG11.4285714285714286No Hit
TAAAGAATCAGAGCGCGTGGGGGAACGAACTGTGGTACCTAGCGTGAGGAC11.4285714285714286No Hit
GATCTAAGTGGAATCTTTTGTGACTGTGTTACTTGCATTTATTTTTATGTC11.4285714285714286No Hit
GGTTTACCAGTGTTTCTCTTCATCCTAATAAACCCTTTCTCTCCCCATCTT11.4285714285714286No Hit
CTACCATCTACATCACACGCATACTCAACTGCTGTCTCTTATACACATCTC11.4285714285714286No Hit
CACTTATGGGGGACCCAGATTACCATACAGTCCGGCCAATGAGAAGTAAGA11.4285714285714286No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAATAAAAAAAAAAAAAAAAT11.4285714285714286No Hit
GCGTTCTTATGGAAACTACTGAGAGTGCTTCAGCTTTCAAACCTGTTCTAA11.4285714285714286No Hit
CAACAATAGAGACACAACAATACCAGTAGCATGTGGGTTGAAGCAAAAGAC11.4285714285714286No Hit
CTCTCATTGTTTGTAACCTTGCAAGTCTTCCCAAGAAATACTTAAACGTGT11.4285714285714286No Hit
ATAGAGGATTGTACACTAGATTTACTAACCCTAACGGGTTTTTAGAACCTG11.4285714285714286No Hit
GTTTCAATGTGGAAACTGTGGAATACAAGAACATTAGTTTCACCGTGTGGG11.4285714285714286No Hit
GGACTGAATTCTTTTGGGATTTATTATCTTTCGGAGAGGATACTATTGAGA11.4285714285714286No Hit
GAACCAGACATTGCATAAATCCTAAGGGACCAACCACATCAAGAAATATAT11.4285714285714286No Hit
TTTCTGGAGTCCTTTAACATTCTTACTGGGATACACTGATCCAGCTAAACC11.4285714285714286No Hit
ATGTGAAGCTTTTGGCACTTCCTTTGTTAGCCAAATCAATGACTTCTTTAG11.4285714285714286No Hit
GTTAAGGGAAGCGTGTGTTAACTTTGTGGACTTGTGCAAGAGAAAGAACGA11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC50.045.021
AACGCTA50.045.010
ACTGTCT50.045.038
ACGCTAT50.045.011
CAACTCA50.045.04
TGATTTA50.045.029
CTCTTGA50.045.025
TTATCAC50.045.033
CTCTTAT50.045.043
CACTGTC50.045.037
TCAACTC50.045.03
TCAACGC50.045.08
CGCTATT50.045.012
GCTATTA50.045.013
AACTCAA50.045.05
ACTCAAC50.045.06
ATTAATT50.045.016
ATTGTCT50.045.020
TAATTGT50.045.018
TTGATTT50.045.028
Produced by FastQC (version 0.11.2)