FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_91.3410000000b2c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_91.3410000000b2c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC24.761904761904762RNA PCR Primer, Index 23 (95% over 21bp)
AACTTCATCAGTCTTCACTTCAACAACTTCTGGGATATTCTTGTCCTCCAA12.380952380952381No Hit
ACAGAGAGCAAGTGATCATCCCTAAGAAGAAGAACAACATCTTCATGTTCG12.380952380952381No Hit
GGATTATATGGTTGAGGAAGATGATGATGGTGTTGGTATGGTACTCTAGGA12.380952380952381No Hit
CACTAATAGTATAGTAGAACCTTCATCAACAGTTTTATCCTTTGGAGATTG12.380952380952381No Hit
GATTAGAACTGTTCATTGATCCCTCAATAATAAGCCTGTCTCTTATACACA12.380952380952381No Hit
GGTAACAGCCTTGGTTCCTTCAGAAACAGCGTGTTTAGCGAGCTCACCAGG12.380952380952381No Hit
TGTCCGAATGGGGAAACCCACCTCGTAAGAGGTATCCGCAAGGATGGGAAC12.380952380952381No Hit
ATCTAGCAGTTTTATGTTGGGGGCTAAACGCCAAGAACATGTTTGCTTAGA12.380952380952381No Hit
GAAGGGTCTGGCTCGTATGCCGAGTTCTGCTAAAAAGAAAGAATGCAAATT12.380952380952381No Hit
ACCCACTTTCGTGCATTTGTCAATGGTGATGTTATTCACTTTTCCTTGTAT12.380952380952381No Hit
TTGTGCGAAAGTGCGCGCGTTTATAGCACGCCGTCTTGCCATGCCCCGTCC12.380952380952381No Hit
GACTTGAAACCGTGTGCTTACAAGTAGTCAGAGCCCGTTAATGGGTGATGG12.380952380952381No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCGGCCCATCTCGTATGCCGTC12.380952380952381No Hit
TGATGATAGTGGTGGAACTTGTAACAACGTGGCTGGTCACTTACAAATGGC12.380952380952381No Hit
GACGAGTATTCAATCAAGATCCAAATCAGCGAAACAGAGAACACAAAGATA12.380952380952381No Hit
CTCCGTGTTGACGTTGACCCCGCCCACCGTCAACGAGCAGACCTTTCGCCC12.380952380952381No Hit
CCTCGGGAGCCTTGCCGTCCAATTCGTCACCAACAAGGTAGCCGGACTTCT12.380952380952381No Hit
TCAAAGAAGCTCATAGATTTGGGGTTAAAGTTCATTTCCTGTCTCTTATAC12.380952380952381No Hit
ATATACCTTCACGTTCATAGAAAAGAACTAGCTTAGACAGGCATAGTAGCC12.380952380952381No Hit
TCTTAATTATGATCTGAACAGCATCCTTTGAGTAATAACGACCATCACCCT12.380952380952381No Hit
CACATAGATGGTCTTGACCTTCAGTTCGTCGATGTCGGCACCGTCGTTGTG12.380952380952381No Hit
ACCCTGGACAGCAGCCTTGGTCCGGTGGATGCCCCCCTGCCGAGGCTGGCC12.380952380952381No Hit
ATGTAGCTAATTGTCTGTTTTGGTTGGCCATTTCTACCACCAATAGTAGCT12.380952380952381No Hit
GCTTGAAGTGATCCAGATCCAGCATCACGAAGGACACCGGCTGGCCCTCGC12.380952380952381No Hit
GGCCGATCAGCTGCGCTTCGTGGAAGACCCCCGCGGCAGCCATGACGAAGC12.380952380952381No Hit
GAAAGAAGGGAGCAAGGTACAACTTGAAATTCACTTTTCATGTTAACAACA12.380952380952381No Hit
CACTTGGGAGGCTCGCATCAAAGTTCTTGTTGGCACTGCAAAGGCACTGGC12.380952380952381No Hit
CCGCGGGTCAGCTCGTCGAGGGAGAAGGAATCGACGCTGACCTTCAGCCCC12.380952380952381No Hit
GTCCTACTCAATTGCAGCAGATGCACTCTGGGAAGGGACTGTAGGACCACA12.380952380952381No Hit
GAAAACATTTGGTTATTTCTCTTTTGCTTTCTTTGATTGGATTCTGTCTCT12.380952380952381No Hit
CCTTAAAGATGTTAACTTCTTCAATAGCGGGAATGGAGTTAACTCCAATTC12.380952380952381No Hit
GGGTAGATTGCATCTTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGA12.380952380952381No Hit
ATTTTCCTCTAATTACCATTTTAGCAGCATCACTCAGGTATTGATCTGTCT12.380952380952381No Hit
GCTTAGAAGCAGCCACCCTTCAAAGAGTGCGTAATAGCTCACTGGTCGAGT12.380952380952381No Hit
ACCGCGCGGGCGGCGGCCGGGCTCCAGTAGTGGGCCAGTGCCGCCTCTGTC12.380952380952381No Hit
ATGCATGACCAGGTTGAAACCCCCGTGACAGGGGGCGGAGGACCGAACCGG12.380952380952381No Hit
GGCTTAGAGGAGGTATTATTGAGCCTTCCTTGATGACTGTCTCTTATACAC12.380952380952381No Hit
ACAATAATGGGTCCATCTGATAGTCCTTATTCAGGCGGTGTCTTTCTCGTA12.380952380952381No Hit
GAGCGAAAGCGAGTCCGAATAGGGCGGCATTAGTACGTCGGGCTAGACTCG12.380952380952381No Hit
AGCCACGGAGCTTGGATACGGTTTCCCGATCGGAGATCCATGGATCACAGA12.380952380952381No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)