##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_4_91.3410000000b2c5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.214285714285715 0.0 0.0 0.0 0.0 0.0 2 33.23809523809524 0.0 0.0 0.0 0.0 0.0 3 34.19047619047619 0.0 0.0 0.0 0.0 0.0 4 35.023809523809526 0.0 0.0 0.0 0.0 0.0 5 34.214285714285715 0.0 0.0 0.0 0.0 0.0 6 33.333333333333336 0.0 0.0 0.0 0.0 0.0 7 34.476190476190474 0.0 0.0 0.0 0.0 0.0 8 34.23809523809524 0.0 0.0 0.0 0.0 0.0 9 34.214285714285715 0.0 0.0 0.0 0.0 0.0 10 35.714285714285715 0.0 0.0 0.0 0.0 0.0 11 35.04761904761905 0.0 0.0 0.0 0.0 0.0 12 36.142857142857146 0.0 0.0 0.0 0.0 0.0 13 36.833333333333336 0.0 0.0 0.0 0.0 0.0 14 35.69047619047619 0.0 0.0 0.0 0.0 0.0 15 36.476190476190474 0.0 0.0 0.0 0.0 0.0 16 35.92857142857143 0.0 0.0 0.0 0.0 0.0 17 34.95238095238095 0.0 0.0 0.0 0.0 0.0 18 36.214285714285715 0.0 0.0 0.0 0.0 0.0 19 35.666666666666664 0.0 0.0 0.0 0.0 0.0 20 37.07142857142857 0.0 0.0 0.0 0.0 0.0 21 37.666666666666664 0.0 0.0 0.0 0.0 0.0 22 36.976190476190474 0.0 0.0 0.0 0.0 0.0 23 35.404761904761905 0.0 0.0 0.0 0.0 0.0 24 36.0 0.0 0.0 0.0 0.0 0.0 25 35.73809523809524 0.0 0.0 0.0 0.0 0.0 26 34.23809523809524 0.0 0.0 0.0 0.0 0.0 27 35.26190476190476 0.0 0.0 0.0 0.0 0.0 28 35.61904761904762 0.0 0.0 0.0 0.0 0.0 29 35.04761904761905 0.0 0.0 0.0 0.0 0.0 30 35.45238095238095 0.0 0.0 0.0 0.0 0.0 31 36.0 0.0 0.0 0.0 0.0 0.0 32 34.785714285714285 0.0 0.0 0.0 0.0 0.0 33 35.38095238095238 0.0 0.0 0.0 0.0 0.0 34 35.54761904761905 0.0 0.0 0.0 0.0 0.0 35 36.404761904761905 0.0 0.0 0.0 0.0 0.0 36 35.785714285714285 0.0 0.0 0.0 0.0 0.0 37 34.80952380952381 0.0 0.0 0.0 0.0 0.0 38 35.142857142857146 0.0 0.0 0.0 0.0 0.0 39 36.214285714285715 0.0 0.0 0.0 0.0 0.0 40 36.45238095238095 0.0 0.0 0.0 0.0 0.0 41 34.214285714285715 0.0 0.0 0.0 0.0 0.0 42 35.285714285714285 0.0 0.0 0.0 0.0 0.0 43 35.976190476190474 0.0 0.0 0.0 0.0 0.0 44 34.38095238095238 0.0 0.0 0.0 0.0 0.0 45 36.333333333333336 0.0 0.0 0.0 0.0 0.0 46 35.642857142857146 0.0 0.0 0.0 0.0 0.0 47 36.45238095238095 0.0 0.0 0.0 0.0 0.0 48 35.666666666666664 0.0 0.0 0.0 0.0 0.0 49 33.357142857142854 0.0 0.0 0.0 0.0 0.0 50 35.333333333333336 0.0 0.0 0.0 0.0 0.0 51 34.54761904761905 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2101 1 7.75 2101 2 7.75 2101 3 2.75 2101 4 3.0 2101 5 3.0 2101 6 7.75 2101 7 2.5 2101 8 6.25 2101 9 3.0 2101 10 4.5 2101 11 4.5 2101 12 10.5 2101 13 4.25 2101 14 3.0 2101 15 7.75 2101 16 0.0 2101 17 7.75 2101 18 7.75 2101 19 3.0 2101 20 1.5 2101 21 1.5 2101 22 0.5 2101 23 7.75 2101 24 4.25 2101 25 1.0 2101 26 7.75 2101 27 3.75 2101 28 7.25 2101 29 7.25 2101 30 3.75 2101 31 7.25 2101 32 7.25 2101 33 9.25 2101 34 7.0 2101 35 1.5 2101 36 7.75 2101 37 9.25 2101 38 7.75 2101 39 4.25 2101 40 7.75 2101 41 12.5 2101 42 6.25 2101 43 4.25 2101 44 9.25 2101 45 9.25 2101 46 7.75 2101 47 1.5 2101 48 5.75 2101 49 7.75 2101 50 10.5 2101 51 6.25 1114 1 -17.25 1114 2 -17.25 1114 3 -8.25 1114 4 3.0 1114 5 -3.0 1114 6 5.75 1114 7 -5.5 1114 8 6.25 1114 9 1.0 1114 10 -3.5 1114 11 -3.5 1114 12 -16.5 1114 13 2.25 1114 14 1.0 1114 15 5.75 1114 16 0.0 1114 17 5.75 1114 18 5.75 1114 19 1.0 1114 20 -0.5 1114 21 -0.5 1114 22 -1.5 1114 23 5.75 1114 24 2.25 1114 25 -1.0 1114 26 5.75 1114 27 3.75 1114 28 7.25 1114 29 7.25 1114 30 3.75 1114 31 7.25 1114 32 5.25 1114 33 1.25 1114 34 5.0 1114 35 -0.5 1114 36 5.75 1114 37 1.25 1114 38 5.75 1114 39 2.25 1114 40 7.75 1114 41 12.5 1114 42 6.25 1114 43 2.25 1114 44 1.25 1114 45 7.25 1114 46 5.75 1114 47 -0.5 1114 48 -2.25 1114 49 5.75 1114 50 -2.5 1114 51 6.25 2215 1 1.75 2215 2 7.75 2215 3 2.75 2215 4 -3.0 2215 5 3.0 2215 6 5.75 2215 7 2.5 2215 8 -18.75 2215 9 -5.0 2215 10 -3.5 2215 11 -3.5 2215 12 -2.5 2215 13 -8.75 2215 14 -5.0 2215 15 5.75 2215 16 0.0 2215 17 5.75 2215 18 5.75 2215 19 1.0 2215 20 -0.5 2215 21 -0.5 2215 22 0.5 2215 23 5.75 2215 24 2.25 2215 25 1.0 2215 26 5.75 2215 27 1.75 2215 28 5.25 2215 29 5.25 2215 30 1.75 2215 31 5.25 2215 32 7.25 2215 33 7.25 2215 34 -6.0 2215 35 -0.5 2215 36 5.75 2215 37 7.25 2215 38 5.75 2215 39 2.25 2215 40 1.75 2215 41 -12.5 2215 42 6.25 2215 43 2.25 2215 44 7.25 2215 45 1.25 2215 46 5.75 2215 47 -0.5 2215 48 3.75 2215 49 5.75 2215 50 8.5 2215 51 6.25 2111 1 7.75 2111 2 1.75 2111 3 2.75 2111 4 -3.0 2111 5 -3.0 2111 6 -19.25 2111 7 0.5 2111 8 6.25 2111 9 1.0 2111 10 2.5 2111 11 2.5 2111 12 8.5 2111 13 2.25 2111 14 1.0 2111 15 -19.25 2111 16 0.0 2111 17 -19.25 2111 18 -19.25 2111 19 -5.0 2111 20 -0.5 2111 21 -0.5 2111 22 0.5 2111 23 -19.25 2111 24 -8.75 2111 25 -1.0 2111 26 -19.25 2111 27 -9.25 2111 28 -19.75 2111 29 -19.75 2111 30 -9.25 2111 31 -19.75 2111 32 -19.75 2111 33 -17.75 2111 34 -6.0 2111 35 -0.5 2111 36 -19.25 2111 37 -17.75 2111 38 -19.25 2111 39 -8.75 2111 40 -17.25 2111 41 -12.5 2111 42 -18.75 2111 43 -8.75 2111 44 -17.75 2111 45 -17.75 2111 46 -19.25 2111 47 -0.5 2111 48 -7.25 2111 49 -19.25 2111 50 -16.5 2111 51 -18.75 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 0.0 26 1.0 27 0.0 28 2.0 29 1.0 30 1.0 31 3.0 32 1.0 33 1.0 34 3.0 35 4.0 36 4.0 37 5.0 38 2.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.714285714285715 28.57142857142857 11.904761904761903 23.809523809523807 2 19.047619047619047 26.190476190476193 26.190476190476193 28.57142857142857 3 14.285714285714285 21.428571428571427 28.57142857142857 35.714285714285715 4 9.523809523809524 21.428571428571427 42.857142857142854 26.190476190476193 5 23.809523809523807 40.476190476190474 30.952380952380953 4.761904761904762 6 38.095238095238095 38.095238095238095 4.761904761904762 19.047619047619047 7 23.809523809523807 35.714285714285715 19.047619047619047 21.428571428571427 8 19.047619047619047 47.61904761904761 28.57142857142857 4.761904761904762 9 26.190476190476193 19.047619047619047 26.190476190476193 28.57142857142857 10 33.33333333333333 23.809523809523807 21.428571428571427 21.428571428571427 11 40.476190476190474 26.190476190476193 16.666666666666664 16.666666666666664 12 23.809523809523807 11.904761904761903 40.476190476190474 23.809523809523807 13 28.57142857142857 9.523809523809524 47.61904761904761 14.285714285714285 14 23.809523809523807 9.523809523809524 28.57142857142857 38.095238095238095 15 19.047619047619047 33.33333333333333 26.190476190476193 21.428571428571427 16 33.33333333333333 14.285714285714285 26.190476190476193 26.190476190476193 17 19.047619047619047 35.714285714285715 21.428571428571427 23.809523809523807 18 14.285714285714285 23.809523809523807 38.095238095238095 23.809523809523807 19 16.666666666666664 23.809523809523807 23.809523809523807 35.714285714285715 20 21.428571428571427 14.285714285714285 38.095238095238095 26.190476190476193 21 19.047619047619047 21.428571428571427 19.047619047619047 40.476190476190474 22 21.428571428571427 33.33333333333333 19.047619047619047 26.190476190476193 23 26.190476190476193 33.33333333333333 16.666666666666664 23.809523809523807 24 26.190476190476193 26.190476190476193 26.190476190476193 21.428571428571427 25 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 26 30.952380952380953 30.952380952380953 23.809523809523807 14.285714285714285 27 23.809523809523807 33.33333333333333 19.047619047619047 23.809523809523807 28 11.904761904761903 23.809523809523807 26.190476190476193 38.095238095238095 29 33.33333333333333 33.33333333333333 16.666666666666664 16.666666666666664 30 19.047619047619047 28.57142857142857 28.57142857142857 23.809523809523807 31 35.714285714285715 9.523809523809524 30.952380952380953 23.809523809523807 32 35.714285714285715 28.57142857142857 16.666666666666664 19.047619047619047 33 35.714285714285715 30.952380952380953 23.809523809523807 9.523809523809524 34 14.285714285714285 30.952380952380953 30.952380952380953 23.809523809523807 35 30.952380952380953 16.666666666666664 26.190476190476193 26.190476190476193 36 21.428571428571427 30.952380952380953 19.047619047619047 28.57142857142857 37 28.57142857142857 28.57142857142857 23.809523809523807 19.047619047619047 38 30.952380952380953 16.666666666666664 28.57142857142857 23.809523809523807 39 16.666666666666664 21.428571428571427 23.809523809523807 38.095238095238095 40 28.57142857142857 26.190476190476193 23.809523809523807 21.428571428571427 41 21.428571428571427 26.190476190476193 23.809523809523807 28.57142857142857 42 21.428571428571427 14.285714285714285 35.714285714285715 28.57142857142857 43 23.809523809523807 19.047619047619047 35.714285714285715 21.428571428571427 44 21.428571428571427 16.666666666666664 38.095238095238095 23.809523809523807 45 23.809523809523807 21.428571428571427 28.57142857142857 26.190476190476193 46 23.809523809523807 26.190476190476193 19.047619047619047 30.952380952380953 47 16.666666666666664 21.428571428571427 30.952380952380953 30.952380952380953 48 19.047619047619047 28.57142857142857 19.047619047619047 33.33333333333333 49 30.952380952380953 26.190476190476193 26.190476190476193 16.666666666666664 50 28.57142857142857 11.904761904761903 23.809523809523807 35.714285714285715 51 16.666666666666664 23.809523809523807 19.047619047619047 40.476190476190474 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 1.0 33 1.0 34 2.5 35 4.0 36 4.0 37 4.0 38 3.5 39 3.0 40 3.0 41 3.0 42 3.0 43 3.0 44 2.0 45 1.0 46 0.5 47 0.0 48 0.5 49 1.0 50 1.5 51 2.0 52 3.5 53 5.0 54 4.0 55 3.0 56 1.5 57 0.0 58 1.5 59 3.0 60 2.5 61 2.0 62 1.0 63 0.0 64 1.0 65 2.0 66 1.5 67 1.0 68 1.0 69 1.0 70 0.5 71 0.0 72 0.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 42.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.61904761904762 #Duplication Level Percentage of deduplicated Percentage of total 1 97.5609756097561 95.23809523809523 2 2.4390243902439024 4.761904761904762 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 2 4.761904761904762 RNA PCR Primer, Index 23 (95% over 21bp) AACTTCATCAGTCTTCACTTCAACAACTTCTGGGATATTCTTGTCCTCCAA 1 2.380952380952381 No Hit ACAGAGAGCAAGTGATCATCCCTAAGAAGAAGAACAACATCTTCATGTTCG 1 2.380952380952381 No Hit GGATTATATGGTTGAGGAAGATGATGATGGTGTTGGTATGGTACTCTAGGA 1 2.380952380952381 No Hit CACTAATAGTATAGTAGAACCTTCATCAACAGTTTTATCCTTTGGAGATTG 1 2.380952380952381 No Hit GATTAGAACTGTTCATTGATCCCTCAATAATAAGCCTGTCTCTTATACACA 1 2.380952380952381 No Hit GGTAACAGCCTTGGTTCCTTCAGAAACAGCGTGTTTAGCGAGCTCACCAGG 1 2.380952380952381 No Hit TGTCCGAATGGGGAAACCCACCTCGTAAGAGGTATCCGCAAGGATGGGAAC 1 2.380952380952381 No Hit ATCTAGCAGTTTTATGTTGGGGGCTAAACGCCAAGAACATGTTTGCTTAGA 1 2.380952380952381 No Hit GAAGGGTCTGGCTCGTATGCCGAGTTCTGCTAAAAAGAAAGAATGCAAATT 1 2.380952380952381 No Hit ACCCACTTTCGTGCATTTGTCAATGGTGATGTTATTCACTTTTCCTTGTAT 1 2.380952380952381 No Hit TTGTGCGAAAGTGCGCGCGTTTATAGCACGCCGTCTTGCCATGCCCCGTCC 1 2.380952380952381 No Hit GACTTGAAACCGTGTGCTTACAAGTAGTCAGAGCCCGTTAATGGGTGATGG 1 2.380952380952381 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCGGCCCATCTCGTATGCCGTC 1 2.380952380952381 No Hit TGATGATAGTGGTGGAACTTGTAACAACGTGGCTGGTCACTTACAAATGGC 1 2.380952380952381 No Hit GACGAGTATTCAATCAAGATCCAAATCAGCGAAACAGAGAACACAAAGATA 1 2.380952380952381 No Hit CTCCGTGTTGACGTTGACCCCGCCCACCGTCAACGAGCAGACCTTTCGCCC 1 2.380952380952381 No Hit CCTCGGGAGCCTTGCCGTCCAATTCGTCACCAACAAGGTAGCCGGACTTCT 1 2.380952380952381 No Hit TCAAAGAAGCTCATAGATTTGGGGTTAAAGTTCATTTCCTGTCTCTTATAC 1 2.380952380952381 No Hit ATATACCTTCACGTTCATAGAAAAGAACTAGCTTAGACAGGCATAGTAGCC 1 2.380952380952381 No Hit TCTTAATTATGATCTGAACAGCATCCTTTGAGTAATAACGACCATCACCCT 1 2.380952380952381 No Hit CACATAGATGGTCTTGACCTTCAGTTCGTCGATGTCGGCACCGTCGTTGTG 1 2.380952380952381 No Hit ACCCTGGACAGCAGCCTTGGTCCGGTGGATGCCCCCCTGCCGAGGCTGGCC 1 2.380952380952381 No Hit ATGTAGCTAATTGTCTGTTTTGGTTGGCCATTTCTACCACCAATAGTAGCT 1 2.380952380952381 No Hit GCTTGAAGTGATCCAGATCCAGCATCACGAAGGACACCGGCTGGCCCTCGC 1 2.380952380952381 No Hit GGCCGATCAGCTGCGCTTCGTGGAAGACCCCCGCGGCAGCCATGACGAAGC 1 2.380952380952381 No Hit GAAAGAAGGGAGCAAGGTACAACTTGAAATTCACTTTTCATGTTAACAACA 1 2.380952380952381 No Hit CACTTGGGAGGCTCGCATCAAAGTTCTTGTTGGCACTGCAAAGGCACTGGC 1 2.380952380952381 No Hit CCGCGGGTCAGCTCGTCGAGGGAGAAGGAATCGACGCTGACCTTCAGCCCC 1 2.380952380952381 No Hit GTCCTACTCAATTGCAGCAGATGCACTCTGGGAAGGGACTGTAGGACCACA 1 2.380952380952381 No Hit GAAAACATTTGGTTATTTCTCTTTTGCTTTCTTTGATTGGATTCTGTCTCT 1 2.380952380952381 No Hit CCTTAAAGATGTTAACTTCTTCAATAGCGGGAATGGAGTTAACTCCAATTC 1 2.380952380952381 No Hit GGGTAGATTGCATCTTCACAACCACTTCAACTTCGCTGAGTCTCGGGAGGA 1 2.380952380952381 No Hit ATTTTCCTCTAATTACCATTTTAGCAGCATCACTCAGGTATTGATCTGTCT 1 2.380952380952381 No Hit GCTTAGAAGCAGCCACCCTTCAAAGAGTGCGTAATAGCTCACTGGTCGAGT 1 2.380952380952381 No Hit ACCGCGCGGGCGGCGGCCGGGCTCCAGTAGTGGGCCAGTGCCGCCTCTGTC 1 2.380952380952381 No Hit ATGCATGACCAGGTTGAAACCCCCGTGACAGGGGGCGGAGGACCGAACCGG 1 2.380952380952381 No Hit GGCTTAGAGGAGGTATTATTGAGCCTTCCTTGATGACTGTCTCTTATACAC 1 2.380952380952381 No Hit ACAATAATGGGTCCATCTGATAGTCCTTATTCAGGCGGTGTCTTTCTCGTA 1 2.380952380952381 No Hit GAGCGAAAGCGAGTCCGAATAGGGCGGCATTAGTACGTCGGGCTAGACTCG 1 2.380952380952381 No Hit AGCCACGGAGCTTGGATACGGTTTCCCGATCGGAGATCCATGGATCACAGA 1 2.380952380952381 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 2.380952380952381 37 0.0 0.0 4.761904761904762 38 0.0 0.0 4.761904761904762 39 0.0 0.0 7.142857142857143 >>END_MODULE >>Kmer Content pass >>END_MODULE