FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_90.3410000000b2b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_90.3410000000b2b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACAACGTCCCTAAAAAGGATGTTAGGTTTCAGTTCATGAACACAGTAA13.125No Hit
CAGACAGACAATGGCGTGATCAGGAAAGGGAGAAAACAAACAAACAAACAA13.125No Hit
TCTTTACCAGTAGCTAAAGTTATTAAATCAGCTGCTGCTAACGCTGAACAC13.125No Hit
CGCCGGCCTTGCGCCGCAATACCTCGATGAGCTTGTCGCGTGGCCAGTCAT13.125No Hit
CCACCGGTCCAACCCGCCAGTCATGCAGACGCGCCAGCACGGCCTGCAGCA13.125No Hit
GTACAATGAAGTCCCTTACTTTGTACATCTAATTTACATTAATATGAATGA13.125No Hit
CCTGCGTGCGGCCGGACTGCTTGCCCTTGGCCGCCTCACGACCTGTCTCTT13.125No Hit
GGAGCGAACAGTGTCGCAATGGCGGCACGGCACATCGATTGAGGGAGTGAT13.125No Hit
ATCAAAAGACTAAGTTTCAGAAGAAGAAAGAGGAAGATTAGGCTACAATTC13.125No Hit
CTATAGAGCTACGTAAAGAAATTATGACTGGATTTGAACAATTTGGCATTA13.125No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC13.125No Hit
ACAAAAGACAAAGGAGTTTTTCAATGGCACTTTCTAAACTAGATAGCCTTT13.125No Hit
GCTGGAACAGCAGCCGCCAATCCCCCGCGGCGATCAGCCCCGAGACTGCCT13.125No Hit
TCACCATGGCGACCGCCCGGCGCCGAGCAAGGTTCACGCTGGCGACACTGC13.125No Hit
GCATTAATCTTCCTAACTTTCCCCTTAGCTTCGTAGTAATGAATCACTGGT13.125No Hit
CTTAGCTGCAGGTCTGTTGGAATTTGCCAGAGGTCCACTCCAGACCCTGTT13.125No Hit
TTCTGAAGCTCTTCCTTTGATTACTTGCAAAAGAAGGAAATAAGACTGTCT13.125No Hit
AATCTAAACTAGGTATCTATTCTTTGCAATCCCGCCTGCAGCTATAAATTC13.125No Hit
GGTATATGCTAATTATCTCATTTCGTTGTCATAATCCACACACCAGCGGTA13.125No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG13.125No Hit
AACCAAATGCGTTTATCTTCCTTGGAATGAAGATCTCAACACTGCTTAAGT13.125No Hit
GATTCGGTTAGCGAGAATTTTATTGAGGATTTTTGCATCGATATTCATAAG13.125No Hit
GGAGCGTGGTGAGATCCTGCGCAAGGCTTTCGAGCTCGTCACCGAGCGCAA13.125No Hit
ATTCAAGGGCAACTCGACCAGGTGCCCTTTTATGAAGCGCAAAACAATTCA13.125No Hit
ATTCCATTCTATTCCATTCTACTCGTGTTGATTCCATTCCTTTCCATTCCA13.125No Hit
GTACTGGCACCTGAGTAGGTCGTTGTTCGTGAAACGATGACTGAATCCGCG13.125No Hit
GGTGAAAGCAACTAAGGCAGAGAAGAAGATCGCCTACGATACCAAGCTACT13.125No Hit
TCCCTGCGGGCAAGTGCCAGTAGGCCGCATGAAGAAGCCAGGATGTAGCGC13.125No Hit
GGGCTGGGGCATCGGCGGCACGGGCCGGGACGTGCCGAGCGCCGCATGGAC13.125No Hit
ATTTATATGCAGGCATCTACAAAGGATAAGCGAGTTAAGACCTTAGTTTTA13.125No Hit
ATGTAGAATATGGTGACAAAACATTAAAAAAAGTGGAAAATGAGAACTGCA13.125No Hit
CCCTATGCTCGTCATGGTCCCCGCTCAGCCCCAGCAACTATCCGGTAGCCG13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)