FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_9.3410000000ad2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_9.3410000000ad2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729141
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC12020.16485151705911477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2000.035.9987418
TATGCCG2200.034.7762843
TGCCGTC2250.034.00347545
CGCTATG2200.033.74881730
CCCACGA2150.033.48719819
CACGAGA2250.031.99887821
ATGCCGT2400.031.87825444
GACTCGC2350.031.59463726
CCGAGCC2400.030.93641714
GAGCCCA2400.030.93641716
AGCCCAC2350.030.63722417
CCACGAG2350.030.63722420
CGTATGC2500.030.59892841
GTATGCC2450.030.30505842
CTCGCTA2450.030.30505828
ACTCGCT2450.030.30505827
TCCGAGC2550.029.9989513
ACGAGAC2400.029.9989522
CTCGTAT2500.029.6989639
TCGCTAT2500.029.6989629
Produced by FastQC (version 0.11.2)