FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_89.3410000000b2ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_89.3410000000b2ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCGAACAGCAGGCGCGCGCTCAATTGCAGCTTGCCGCTGTCTCTTATA11.8867924528301887No Hit
CATGACATCAAACTAAAACTCTTTTGTCTTTTTTTCCCTGTCTCTTATACA11.8867924528301887No Hit
CTTATAAGCAGTGATTTTGAATGGTTTATGTCTGTCTCTTATACACATCTC11.8867924528301887No Hit
GCATAAGCATGATTAATTTTTTCAGTTGTACCTCTTCCTACGATTTCTGCT11.8867924528301887No Hit
GGTCAACACTAGTCAAAGAACTAAGAAAAGGATAAAGGAAAATGAGTCCAA11.8867924528301887No Hit
ATCTAAGAAACTTTGATTTCTCCTTTTGTGTTTCTCATTGATTTGCAATTG11.8867924528301887No Hit
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT11.8867924528301887RNA PCR Primer, Index 23 (95% over 23bp)
TGCCAGTCCCGAGCCACTGCTCTAGATTTTGTCCCTGTCAAACACAAGCAA11.8867924528301887No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTCTGTCTCTTATACACATCT11.8867924528301887No Hit
TCGTAAAACTCTGTTATTAGGGAAGGACGGTACCTAATCCTGTCTCTTATA11.8867924528301887No Hit
CATACCAACAATTTGGAAATCAATCATACGCTCATCAACATCTACGTTAAC11.8867924528301887No Hit
GGTCACCTCATCCACTCCTCACCCACCCGAAGGCGGTTAAGAGAAGCTTCA11.8867924528301887No Hit
TGTACAGACAGACTTTTGGGGTGCTAAGATCCAAAGTCGAGAGGGAAACAG11.8867924528301887No Hit
CCTTTAGCTTCCTCAATATTTCCTATTCTCCTTTCTAACTGTCTCTTATAC11.8867924528301887No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTACTGTCTCTTATA11.8867924528301887No Hit
GAACATATGCACATTTCCTTCAATTGTTCAATATGTTATACCTGTCTCTTA11.8867924528301887No Hit
CTGGCAGAAGTAATCATATGTGTTCTTGGGTTGTAATAAGTGTTTGTAGCT11.8867924528301887No Hit
CTTTTAATTTTATCCGTCATCGTAGCTAAACTGGTATAGAACCAACCATTG11.8867924528301887No Hit
CTCTAAATGATAAGGTTTAGTGGACTTCTCGCGACGTCGCGGGCCTGTCCC11.8867924528301887No Hit
GGGTCGAGAGCCTCACGGCGCTCGTCGAGCATCACGACGGCCAGCTTGAGA11.8867924528301887No Hit
CTGTTAGAGCTCTCTTCAACCTGCTCATGGCTAGATCGATCGGTTTCGGGT11.8867924528301887No Hit
GCCTTATCCCATCCAATCCTTAAACTCGACCTGAATATGTACTGTCTCTTA11.8867924528301887No Hit
GTCCTCCAACAATAAAGCTTACACAAGTTGTATACAGCCTGTCTCTTATAC11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC11.8867924528301887No Hit
GAAAAAGAAAGACGAAGCTTCGTTCCAAAGTGTTCACTTCTTTTTCGCCAG11.8867924528301887No Hit
GGCCAGCACGTGTCGACCACCCGCAGCAAAGGCAAGACCCAGACGACAACC11.8867924528301887No Hit
TTGTAAGACTGCTTGACTCAAATCTAATGCTGTCTCTTATACACATCTCCG11.8867924528301887No Hit
GTGAAAAAGTGAGGAGATTTGGTAAATTCTTGCCACTTGGGTTTTTAAGCT11.8867924528301887No Hit
CAATTGTTTTCTTCTCCTACGGGTACTGAGATGTTTCACTTCTGTCTCTTA11.8867924528301887No Hit
GTACGGGCCCACAGTCTTAAATTTTCAATCTCTTTAAAATATCCATCTCTT11.8867924528301887No Hit
GCCCAGGAAGACGGGCGCCTGCGCGGCAGCGACCCGGCGTTTGCCGCCCAC11.8867924528301887No Hit
ATTATAAGAGTAGCAAAAGTGAGAGACAAAAACGAAATAACAAGGTTTATG11.8867924528301887No Hit
CATCAAGACTCGGTGCTGCATCACAATCTATAAGTACTTCATTTTAGATAA11.8867924528301887No Hit
GTATCAACGCAGAGTACGGGATGACGGCCTTCGGGTTGTAAACCGCTTTCG11.8867924528301887No Hit
CATCATGACCGCTCACGTCGGCTGTCTGGAGCTTTGCCGGGCGATGGCCAA11.8867924528301887No Hit
GAGTAGTGGAAGTAGTGCAAGTAGAGAGATTCTGGTGCAGCATTTGCTTGT11.8867924528301887No Hit
CTTATACACATCTCAGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC11.8867924528301887No Hit
CCTTAGAATTAGTTCTGTGAGGCCTCTCTACATCCCTTGAGTCTTTCTTTA11.8867924528301887No Hit
GAGTTATCGTTTCACAATTAGTACGTTCACCATCAGTGTATTTTAACTGTC11.8867924528301887No Hit
TTATTGTACAAGAGGAGAAGAATCAGGGAAAGGTAGTCAAACAGAAGCCGC11.8867924528301887No Hit
CCCGTGAACATATCGATGCCGCCGCATCCCGCCGTGACCTTGGGCAGCTGC11.8867924528301887No Hit
TATCATTACATCGTCATACATGATACCCAACACCATCCATCATATCACTGT11.8867924528301887No Hit
AAATTCTTCAAACAAAACATATCCTCGAATTCAAAATCTTCAAACGCAACT11.8867924528301887No Hit
CACACGTATAATTTCATCAATGTGGTCTAAAGCAATTCTTAAGCCTTCTAA11.8867924528301887No Hit
GTCATATAGGTCTTAGTTCATTAAGCCTTCGTCTTGGGTAAAAACTGTCTC11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC11.8867924528301887RNA PCR Primer, Index 45 (95% over 21bp)
GTGCTTTCACTTTATGGCTTGTCTTTTGAAAAGAGTTGTAAGTGGAATTTA11.8867924528301887No Hit
AATGCAAACAGACCAGTGACTTGACTTGAGATGACATTTTAGACAACTATT11.8867924528301887No Hit
ATACTTGAATGACCTGAAAATCAGTTTTCCCAATTCCAGGTCTTAGCAGGT11.8867924528301887No Hit
CTACTAGAGGCATGAGACAAAGAAGGCTTGATTGGTGATTTACCTGTCTCT11.8867924528301887No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCTGTCTCTTATACACA11.8867924528301887No Hit
CATATAGACTAGAGTTTTAACTTTTAAGCTCAAAGACAAAAGAAACGAGCA11.8867924528301887No Hit
CGTTGCTCGATCCGCCAGCCGAACTGGCGCTCGCCGGTCGTACCCGCGGTG11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA50.045.039
ACAGACC50.045.08
CCAGTGA50.045.013
AAACAGA50.045.06
ACTTGAG50.045.024
ACTTGAC50.045.019
TGCAAAC50.045.03
ATGACAT50.045.031
CAACTAT50.045.044
TGACATT50.045.032
CATTTTA50.045.035
ACATTTT50.045.034
TTGAGAT50.045.026
GATGACA50.045.030
GACATTT50.045.033
TTTTAGA50.045.037
GCAAACA50.045.04
AACTATT50.045.045
GACAACT50.045.042
TTGACTT50.045.021
Produced by FastQC (version 0.11.2)