FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_88.3410000000b29f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_88.3410000000b29f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGAGGTACGTGAGTTCGGTTTAATACGACGTGAGTCAGTATCTGT11.5625No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGAAAACTT11.5625No Hit
GATCAGGGCTATACCGGTGCACGGGCAGCCAATGCCGCAGCCGCCCATCTG11.5625No Hit
ATTGGAAACCAAGAAAAGATCCCACAAACTCTCAAGGAGAAATTCTGTCTC11.5625No Hit
GGTTAGTTTTACCCTACTGATGGAGGGTTATCGCAATAGCTGTCTCTTATA11.5625No Hit
GGACAGGACCGTCTATAACATACTACTATACCTGCACATTCACATTCACAG11.5625No Hit
GTGTTATAAGGTGTACTTTCGGAGGATTCATGGATCACATTTCTAAGCTCA11.5625No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGAGATATCTCGTATGCCGTC11.5625No Hit
ATCATAGCATAAACATAGACAGTCTTTACACCTGTCTCTTATACACATCTC11.5625No Hit
GTTCAAACTAATGCAACTGTGACGCTCTCTGATGACAAGAAGACTGCAACA11.5625No Hit
TATATTGATGGAGAAATCAAGGTTTGCTCTTCTACAACTGTCTCTTATACA11.5625No Hit
TTCATGTGCTGTGCAACTTGTCATAATCTGTATGCTCAACTCTCAAAAAAA11.5625No Hit
TGTTCAAGCATGCACCACATATGTTGATGTGATCGTCTCTGTCTCTTATAC11.5625No Hit
ACAATATTTGTTCTCGAAAGTCTGAGTTTCCGTGTACTCTCCAAATTTATG11.5625No Hit
GATCTACGCCGAGCAACTGCGTCAGGACGGGCAGGGCACGGTGATCGTGGA11.5625No Hit
AGATATACCTCTCTTGGGCATATACCCAGAAGATGTTCCAACTAGTAAGAA11.5625No Hit
CTCGAATAGACTGAAACCCATGTCATCATCGGATTCTTCCTGTCTCTTATA11.5625No Hit
AGACTTAGATTGGACTAATAGTAAGGTAACGTCATTATCCCTGTCTCTTAT11.5625No Hit
GTACTAAGATGTTTCAGTTCCCTGCGTTAGCCTGTCTCTTATACACATCTC11.5625No Hit
ATGATAAAGTATGCTTTACTGAAAGATTGATAAATCGATCAGTTACGCAAG11.5625No Hit
TTCATATGGCGTTCATGTTCACTCTAAGAAATCTGTGATTGGTTGAAAACT11.5625No Hit
GCGCCCAATTCAGCAAAGTCGCAGACATCCAGGATTCAAGCACGCCACATG11.5625No Hit
GATATGATCGTCGTGAAAATGCGTACACAAGCGTATTGGTCATGAGAATCT11.5625No Hit
TGTTGGACCACTTTCCCTGATGCTTAAGTTCTTCTTGACCTCAACGTAAGC11.5625No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTTCCTCTTTT11.5625No Hit
ACATATTACCGAAGCTGTGGATACCTTTATAGGTATGGTAGGAGAGCTGTC11.5625No Hit
TCTGTGTGTATATGCACAGGTGTATGTATACGTGTGTGTTGGTGTATTTGT11.5625No Hit
GATTTGAAGCGCTTTCCAGGACCGGGCCGGGAGACGAGAAGCCCGCGGCGG11.5625No Hit
GTCCTTCAGCTGAATATGTTCAATTTCAAGATGAAGTGGCTAAAGCAAAGA11.5625No Hit
ATGACCAAGGAGTTTTTCCATGAAGACAGCTTTACGATCCGCGCTTACTGC11.5625No Hit
CATTCTTCATTTTGTAGGTAGTTGTTGTGGTAGCTGTCTCTTATACACATC11.5625No Hit
GTTGTAATTCTCCAACATCACATCCATGTACAATGTCCTCTGAGCAAAATT11.5625No Hit
AGCTGGAGCTCATAGCTGCTTTGGTTCTCTCCACAGCATAGCATAATCAGC11.5625No Hit
GATAAGTGGTGTACGTGCTTCATCAATTAAAATTGAGTCGACCTCATCAAC11.5625No Hit
TTCTTGCGGTCAACCTCGAACACAGCGAGATCCGTAATAATCCTGTCTCTT11.5625No Hit
CTTACAGGACTTTCACCTTCTATGGTCAATCTCTGTCTCTTATACACATCT11.5625No Hit
GGAGGAGAATCTATTTATGGAGGATTCTTTGAAGATGAGAGTTTTGCTGTT11.5625No Hit
GTCTTTCGCTACTCATACCGGCATTCTCACTTCTATGCGCTCCAGCACTCC11.5625No Hit
CCAAAGAATTAGTGTAAATAGTTTACGGTTTGGACTACTCAGGTAGCTAAT11.5625No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCTG11.5625No Hit
GCATGATTTTTAAAATATGTTTCCACTCACGTTAAATTTTATCTCTCAGTG11.5625No Hit
GTTGAGAAGAAGTATGCTGACCTGATTAAGAAAGTCTAACCTTAGGTACTG11.5625No Hit
AATCAGGACATCAAGACATGATTGCCATCCTAAAATCCTGTCTCTTATACA11.5625No Hit
ATCCTACTGCGTCCCCCCATCGATTAAAACGATACTAGGTGGTACAGGAAT11.5625No Hit
GATAAGTACTGAAAGCATATCAAGTGCGAAACAGATCTTCTGATATTCTTT11.5625No Hit
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT11.5625No Hit
GAATCGTTTTTCCTCTGTCGAACGCAGTTCCACCTCGCCGTGTCGGACTCT11.5625No Hit
GCTGGGGCGAGCCGCCGGCAATGCCCACGCTGATGCGCACCACCAGGCCCG11.5625No Hit
ATTGATAGGTTTATTTCGCACTTTAGAAAAAGTTTGGAAAGAAAAGTAGAC11.5625No Hit
TAGTTTTACTGGCCGTATAGTTGCCTTCCTACAAATCCTGTCTCTTATACA11.5625No Hit
GGTCACCTACTGAAGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGCC11.5625No Hit
CTCCAGTTAATTCCTGTAAGAACAGCCTGATGTAAAATGCAGCAAACCTGA11.5625No Hit
GTCCAGAACGTGTCGTCGGAACTGCTCGAAGGCACGGTCCCCGAGGTCACC11.5625No Hit
GCGATGATGCCTTCGCGTTCCAGTTGCGACAGGCGGCTGGCGCTCGACTGC11.5625No Hit
GCAATATCCTGTGATCCGGCGATATCTGAATGGGGAAACCCACTTGTCATA11.5625No Hit
GTTAATAGACATTGTGCAGTGTTTTCCAGAGAAGCACTCATTCTTTTAGTT11.5625No Hit
CTACAAAAGAGGTTATGGAAAGCTGAACCACCAGAGGATAGCACTTACTGA11.5625No Hit
CATTGTACATGGATGTGATGTTGGAGAATTACAACAATCTGTTGTTTGTGG11.5625No Hit
CCTCGCCACCACTCGATTGAGTGGTTCGTTTCGAAAAGCGCCCAGTTGGGC11.5625No Hit
GCCTTGCAGCGTGCTGATGGACGCCTCGATGAAGTTGGCTTGTCGGAAGGT11.5625No Hit
CGCATCCACAGTACCTTTGTTGGCAACCTAGCTTCACGGGTACCCTTCCTC11.5625No Hit
GAGTTGAACCAGTGGCAGTGATGCATAATGGATCCACCTACAAGCGTCATC11.5625No Hit
CTCAAAACACAACATAGATAAATTTACCCTTTCGAAAAACTCATTCTTCGA11.5625No Hit
CCTCACATTAGATCTAGTTACTGTGGTATGGCTAATACCTGTCAACATTTG11.5625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACAT50.045.039
TTCACAG50.045.045
TGCACAT50.045.033
CTATACC50.045.026
CTATAAC50.045.013
TATACCT50.045.027
GACAGGA50.045.02
AGGACCG50.045.05
GGACCGT50.045.06
CCTGCAC50.045.031
TATAACA50.045.014
ACCGTCT50.045.08
CCGTCTA50.045.09
GGACAGG50.045.01
TCTATAA50.045.012
ATAACAT50.045.015
ACCTGCA50.045.030
ACATACT50.045.018
GACCGTC50.045.07
TAACATA50.045.016