FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_87.3410000000b282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_87.3410000000b282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94698
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC1070.11299077066041523RNA PCR Primer, Index 23 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA700.045.069841
CATATAC300.005124588529.9989413
GTATCAA1400.024.1062952
AGCCAGG600.00646878918.7493386
CAACGCA2351.8189894E-1216.2760224
TACGGGA1004.118E-415.7494464
AGAGTAC2456.548362E-1114.69335910
CAGAGTA2509.094947E-1114.3994929
TATCAAC2551.2187229E-1014.1395581
ATCAACG2551.2551027E-1014.1171492
TCAACGC2551.2551027E-1014.1171493
AACGCAG2601.70985E-1013.8456665
GCAGAGT2601.70985E-1013.8456668
ACGCAGA2652.3101165E-1013.5844276
CGCAGAG2754.1472958E-1013.0904477
AGTACGG2704.443791E-912.4995612
GAGTACG2755.838956E-912.27229411
GTACGGG2951.6529157E-811.44027413
TACGGGG1450.006748512410.86168714
Produced by FastQC (version 0.11.2)