##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_4_86.3410000000b275.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9896 Sequences flagged as poor quality 0 Sequence length 51 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.90774050121261 38.0 38.0 38.0 32.0 38.0 2 36.852970897332256 38.0 38.0 38.0 32.0 38.0 3 36.931386418755054 38.0 38.0 38.0 32.0 38.0 4 37.01212611156023 38.0 38.0 38.0 32.0 38.0 5 37.00090945836702 38.0 38.0 38.0 32.0 38.0 6 38.981911883589326 40.0 40.0 40.0 38.0 40.0 7 38.900970088924815 40.0 38.0 40.0 38.0 40.0 8 38.903092158447855 40.0 38.0 40.0 38.0 40.0 9 38.96160064672595 40.0 38.0 40.0 38.0 40.0 10 38.9000606305578 40.0 38.0 40.0 38.0 40.0 11 38.90208164915117 40.0 38.0 40.0 38.0 40.0 12 38.87489894907033 40.0 38.0 40.0 38.0 40.0 13 38.89329021827001 40.0 38.0 40.0 38.0 40.0 14 38.881568310428456 40.0 38.0 40.0 38.0 40.0 15 38.87095796281326 40.0 38.0 40.0 38.0 40.0 16 38.87833468067906 40.0 38.0 40.0 38.0 40.0 17 38.84913096200485 40.0 38.0 40.0 38.0 40.0 18 38.87469684721099 40.0 38.0 40.0 38.0 40.0 19 38.89500808407438 40.0 38.0 40.0 38.0 40.0 20 38.83761115602263 40.0 38.0 40.0 38.0 40.0 21 38.88682295877122 40.0 38.0 40.0 38.0 40.0 22 38.83811641067098 40.0 38.0 40.0 38.0 40.0 23 38.849029911075185 40.0 38.0 40.0 38.0 40.0 24 38.84246160064673 40.0 38.0 40.0 38.0 40.0 25 38.84680679062247 40.0 38.0 40.0 38.0 40.0 26 38.8444826192401 40.0 38.0 40.0 38.0 40.0 27 38.8248787388844 40.0 38.0 40.0 38.0 40.0 28 38.73807599029911 40.0 38.0 40.0 38.0 40.0 29 38.71149959579628 40.0 38.0 40.0 38.0 40.0 30 38.751010509296684 40.0 38.0 40.0 38.0 40.0 31 38.72938561034761 40.0 38.0 40.0 38.0 40.0 32 38.706345998383185 40.0 38.0 40.0 38.0 40.0 33 38.698464025869036 40.0 38.0 40.0 38.0 40.0 34 38.6696645109135 40.0 38.0 40.0 38.0 40.0 35 38.70796281325788 40.0 38.0 40.0 38.0 40.0 36 38.628839935327406 40.0 38.0 40.0 38.0 40.0 37 38.63106305578011 40.0 38.0 40.0 38.0 40.0 38 38.618128536782535 40.0 38.0 40.0 38.0 40.0 39 38.567299919159254 40.0 38.0 40.0 38.0 40.0 40 38.60711398544866 40.0 38.0 40.0 38.0 40.0 41 38.62045270816491 40.0 38.0 40.0 38.0 40.0 42 38.61802748585287 40.0 38.0 40.0 38.0 40.0 43 38.582558609539205 40.0 38.0 40.0 38.0 40.0 44 38.54951495553759 40.0 38.0 40.0 38.0 40.0 45 38.49302748585287 40.0 38.0 40.0 38.0 40.0 46 38.54345189975748 40.0 38.0 40.0 38.0 40.0 47 38.48272029102668 40.0 38.0 40.0 38.0 40.0 48 38.462813257881976 40.0 38.0 40.0 38.0 40.0 49 38.451495553759095 40.0 38.0 40.0 38.0 40.0 50 38.46372271624899 40.0 38.0 40.0 38.0 40.0 51 37.9926232821342 40.0 38.0 40.0 38.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.9079362288522006 2201 2 1.1994384971431202 2201 3 0.19926868837108458 2201 4 0.47447923680690707 2201 5 1.0373371594758112 2201 6 0.7009704735892441 2201 7 0.6954486643468059 2201 8 1.0427520624477893 2201 9 0.9942046903528663 2201 10 0.7366051781922422 2201 11 0.7900267909120586 2201 12 1.0593600165690518 2201 13 0.927911295253601 2201 14 0.5237473086822462 2201 15 0.8900099653770042 2201 16 0.9461687520613893 2201 17 0.4657853989056804 2201 18 0.7421979966708392 2201 19 0.417934497200541 2201 20 0.6649807095155822 2201 21 0.2583756507832504 2201 22 0.9036745110915589 2201 23 0.7930960168733492 2201 24 0.7928136765839966 2201 25 -0.008669340501050726 2201 26 -0.2454475398880973 2201 27 0.3982286017055401 2201 28 0.714324130461911 2201 29 0.8899637503449256 2201 30 0.5168402755628208 2201 31 0.7085180391530912 2201 32 0.28125875719299387 2201 33 -0.2733305420472689 2201 34 0.5223816310431033 2201 35 0.4735172368964484 2201 36 0.6441436713011655 2201 37 0.4504781821635433 2201 38 0.8126909638053874 2201 39 0.7171300957545839 2201 40 0.02536351173819895 2201 41 0.023697359974704568 2201 42 0.7253908605235324 2201 43 0.4186950218683947 2201 44 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0.3579225345249313 1214 4 0.18601769834536697 1214 5 1.0373371594758112 1214 6 0.8836627812815507 1214 7 0.9069871258852658 1214 8 -0.019747937552210715 1214 9 0.2442046903528663 1214 10 0.6692974858845488 1214 11 -0.05612705524178807 1214 12 0.5593600165690518 1214 13 0.20675744909975435 1214 14 0.5141319240668594 1214 15 0.44770227306931076 1214 16 0.6000149059075426 1214 17 0.23501616813644688 1214 18 -0.10876354179070091 1214 19 -0.5291808874148458 1214 20 0.6265191710540421 1214 21 0.9314525738601702 1214 22 0.6825206649377122 1214 23 0.9757883245656558 1214 24 0.9755059842763032 1214 25 0.9432537364220295 1214 26 0.6103216908811362 1214 27 0.13861321709015328 1214 28 -0.1702912541534758 1214 29 0.4476560580372322 1214 30 0.632224890947434 1214 31 0.6700565006915511 1214 32 0.17548952642376037 1214 33 0.438207919491191 1214 34 1.0127662464277165 1214 35 0.9927480061272149 1214 36 0.663374440531932 1214 37 0.5947089513943098 1214 38 1.0530755791900006 1214 39 0.9575147111391971 1214 40 0.7561327425074325 1214 41 0.9852358215131645 1214 42 0.9946216297542989 1214 43 0.9667719449453145 1214 44 0.9345854037254142 1214 45 0.6812710391173908 1214 46 0.9225799204423666 1214 47 0.6903423289440624 1214 48 0.866135352905502 1214 49 -1.1327720017662912 1214 50 0.1961015717437462 1214 51 1.0186709113470087 1215 1 1.0151406942247192 1215 2 0.8464973206725332 1215 3 0.3395401815837573 1215 4 0.58307652187478 1215 5 0.6843959830052242 1215 6 0.6557216048109638 1215 7 1.0393400670617368 1215 8 0.9251050036242603 1215 9 0.4059693962352213 1215 10 0.6913563094139619 1215 11 0.590931768287625 1215 12 0.9417129577455228 1215 13 0.9641103902762254 1215 14 0.9038378064198014 1215 15 0.7271140377751948 1215 16 0.8647207882604846 1215 17 0.5291338151952729 1215 18 0.6155011640916541 1215 19 0.4450838184675092 1215 20 1.008872112230513 1215 21 0.9461584562131122 1215 22 0.8222265472906543 1215 23 0.7478471480950688 1215 24 0.7475648078057162 1215 25 0.7153125599514425 1215 26 0.8676746320576072 1215 27 0.6606720406195663 1215 28 0.9767675693759372 1215 29 0.4917737050960582 1215 30 0.308695479182731 1215 31 0.46417414775037713 1215 32 0.8151954087767024 1215 33 0.482325566550017 1215 34 0.31423683466301355 1215 35 -0.051369640931611116 1215 36 0.825139146414287 1215 37 0.9844148337472518 1215 38 0.9574873438958846 1215 39 0.5089852993744941 1215 40 1.0208386248603745 1215 41 0.5440593509249325 1215 42 0.788739276813125 1215 43 -1.1876398197605695 1215 44 0.6037030507842402 1215 45 -0.6275524902943772 1215 46 -0.10683184426351744 1215 47 0.3668129171793595 1215 48 -1.045629352976853 1215 49 0.25325740999841173 1215 50 0.49021921880257224 1215 51 0.9451414995823058 1212 1 0.30925834128354524 1212 2 0.49355614420194627 1212 3 -0.01340099488683677 1212 4 -0.887511713419336 1212 5 -0.37442754640654385 1212 6 0.18513336951684778 1212 7 0.03934006706173676 1212 8 0.21922265068308633 1212 9 0.17067527858816334 1212 10 -0.014526043527219201 1212 11 0.12034353299350897 1212 12 -0.23475763048977427 1212 13 -0.3300072567826007 1212 14 0.08030839465509132 1212 15 0.02123168483401372 1212 16 0.15883843531931063 1212 17 0.058545579901149836 1212 18 -1.796263541790701 1212 19 -0.025504416826613863 1212 20 0.30298975928933913 1212 21 -0.7597238967280617 1212 22 0.3516383119965383 1212 23 0.15961185397742383 1212 24 0.7475648078057162 1212 25 -0.1670403812250285 1212 26 -0.1323253679423928 1212 27 -0.045210312321614765 1212 28 0.03559109878769817 1212 29 -0.7435204125509998 1212 30 0.42634253800626 1212 31 0.6994682653974351 1212 32 -0.8906869441644716 1212 33 0.952913801844133 1212 34 -0.8622337535722835 1212 35 0.536865653186041 1212 36 0.707492087590758 1212 37 0.8667677749237228 1212 38 0.36925204977823256 1212 39 0.5089852993744941 1212 40 -0.3321025516102125 1212 41 -1.8088818255456616 1212 42 -1.9171430761280561 1212 43 -0.5405809962311565 1212 44 -0.6315910668628177 1212 45 -0.5099054314708411 1212 46 -0.5185965501458654 1212 47 -0.8684812004677056 1212 48 -1.045629352976853 1212 49 -1.1585072958839362 1212 50 -1.6862513694327248 1212 51 -1.7019173239471073 1213 1 -0.9848593057752808 1213 2 -0.8005615028568798 1213 3 -0.9545774654750687 1213 4 -0.6639823016546345 1213 5 -0.5626628405241902 1213 6 -2.391337218718448 1213 7 -0.44301287411473567 1213 8 -0.5572479375522121 1213 9 -0.07246197631380369 1213 10 -0.4223691807821197 1213 11 -0.9227937219084552 1213 12 -1.5406399834309497 1213 13 -0.7849092175669128 1213 14 -1.460868075933142 1213 15 -0.8022977269306892 1213 16 -1.0333184274257903 1213 17 -0.29831716519688456 1213 18 -0.5962635417906981 1213 19 -0.16668088741484866 1213 20 0.19318583772071207 1213 21 -1.551880759473164 1213 22 -0.7424793350622849 1213 23 -0.8992116754343442 1213 24 -2.1661606823903625 1213 25 -1.531746263577972 1213 26 -0.21467830911886665 1213 27 -0.3197201162431824 1213 28 -2.0036245874868115 1213 29 -0.669010608629435 1213 30 -0.7344417757192332 1213 31 -0.696610165975116 1213 32 -1.2828438069095753 1213 33 -0.14512541384214472 1213 34 -2.395567086905615 1213 35 -0.3572519938727865 1213 36 -5.319958892801402 1213 37 -3.5802910486056874 1213 38 -0.7719244208100022 1213 39 -2.0674852888608015 1213 40 -2.143867257492566 1213 41 -2.914764178486834 1213 42 -1.638711703579034 1213 43 -1.7248947217213555 1213 44 -1.557081262941253 1213 45 -2.768728960882612 1213 46 -1.9107534128909691 1213 47 -3.209657671055936 1213 48 -1.633864647094498 1213 49 -1.836938668432957 1213 50 -1.537231761589588 1213 51 -1.497995755319657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 6.0 24 9.0 25 10.0 26 22.0 27 18.0 28 53.0 29 59.0 30 66.0 31 96.0 32 125.0 33 151.0 34 173.0 35 219.0 36 347.0 37 553.0 38 1179.0 39 6808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45914751442746 18.39627417231953 10.944618811379973 25.19995950187304 2 20.260711398544867 19.24009700889248 32.7303961196443 27.76879547291835 3 22.877930476960387 21.715844785772028 25.808407437348425 29.59781729991916 4 17.178658043654004 16.350040420371865 30.2445432497979 36.22675828617623 5 20.462813257881972 32.35650767987065 28.506467259498784 18.674211802748587 6 35.559822150363786 31.436944219886826 15.622473726758287 17.38075990299111 7 32.4373484236055 25.909458367016974 17.774858528698463 23.87833468067906 8 28.102263540824573 29.668552950687143 17.33023443815683 24.898949070331447 9 26.687550525464832 13.136620856911884 18.45189975747777 41.72392886014551 10 21.402586903799513 24.151172190784155 26.01050929668553 28.4357316087308 11 40.076798706548104 19.21988682295877 19.452303961196442 21.251010509296687 12 26.172190784155212 21.10953920776071 24.090541632983022 28.627728375101054 13 34.074373484236055 17.2696038803557 20.78617623282134 27.869846402586905 14 24.767582861762328 17.28981406628941 22.09983831851253 35.84276475343573 15 29.264349232012936 22.433306386418757 19.54324979789814 28.75909458367017 16 28.981406628940988 22.44341147938561 20.301131770412287 28.274050121261112 17 27.970897332255458 22.48383185125303 21.655214227970898 27.89005658852061 18 28.344785772029102 21.37227162489895 22.23120452708165 28.0517380759903 19 30.41632983023444 20.957962813257883 21.695634599838318 26.93007275666936 20 28.80962004850445 21.705739692805174 21.614793856103475 27.869846402586905 21 29.011721907841554 20.59417946645109 21.64510913500404 28.748989490703313 22 28.35489086499596 21.917946645109136 21.119644300727565 28.60751818916734 23 27.849636216653195 21.958367016976556 22.15036378334681 28.041632983023447 24 28.526677445432497 21.513742926434922 22.36257073565077 27.59700889248181 25 28.73888439773646 20.998383185125302 21.018593371059012 29.244139046079226 26 28.637833468067907 22.16046887631366 21.473322554567503 27.72837510105093 27 27.89005658852061 21.453112368633793 22.221099434114794 28.4357316087308 28 28.486257073565074 21.39248181083266 20.796281325788197 29.324979789814066 29 29.193613581244946 22.120048504446242 20.685125303152788 28.001212611156024 30 27.42522231204527 21.998787388843976 22.78698464025869 27.789005658852062 31 28.20331447049313 20.685125303152788 22.342360549717057 28.769199676637026 32 27.314066289409862 21.695634599838318 22.09983831851253 28.890460792239285 33 28.092158447857717 22.109943411479385 21.766370250606307 28.03152789005659 34 28.556992724333064 21.00848827809216 22.29183508488278 28.142683912691997 35 28.65804365400162 21.341956345998383 22.382780921584477 27.617219078415523 36 27.314066289409862 21.746160064672594 21.958367016976556 28.981406628940988 37 28.263945028294263 21.857316087308003 21.059013742926435 28.819725141471302 38 27.35448666127728 22.07962813257882 22.625303152789005 27.94058205335489 39 27.677849636216656 21.443007275666936 22.635408245755862 28.243734842360553 40 28.65804365400162 21.210590137429264 21.81689571544058 28.314470493128535 41 27.55658852061439 21.382376717865803 21.806790622473727 29.254244139046083 42 27.9607922392886 22.029102667744542 21.422797089733226 28.58730800323363 43 28.698464025869036 21.22069523039612 21.786580436540014 28.294260307194826 44 26.899757477768794 21.81689571544058 22.78698464025869 28.49636216653193 45 28.162894098625706 21.68552950687146 21.806790622473727 28.344785772029102 46 28.193209377526273 21.099434114793855 21.73605497170574 28.97130153597413 47 27.000808407437347 21.443007275666936 22.706143896523848 28.85004042037187 48 28.37510105092967 21.574373484236055 22.008892481810832 28.041632983023447 49 28.668148746968473 20.715440582053354 22.241309620048504 28.37510105092967 50 27.5767987065481 20.796281325788197 22.685933710590138 28.940986257073565 51 26.78860145513339 22.221099434114794 22.120048504446242 28.870250606305575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 2.0 9 1.0 10 0.0 11 1.5 12 3.0 13 3.5 14 4.0 15 6.0 16 8.0 17 8.5 18 9.0 19 13.0 20 17.0 21 18.0 22 19.0 23 22.5 24 26.0 25 32.5 26 51.0 27 63.0 28 73.0 29 83.0 30 91.5 31 100.0 32 125.5 33 151.0 34 164.0 35 177.0 36 192.5 37 208.0 38 232.5 39 257.0 40 282.5 41 308.0 42 317.5 43 327.0 44 325.5 45 324.0 46 372.0 47 420.0 48 466.5 49 513.0 50 468.0 51 423.0 52 455.5 53 488.0 54 493.0 55 498.0 56 509.5 57 521.0 58 530.0 59 539.0 60 544.5 61 550.0 62 562.5 63 575.0 64 564.0 65 553.0 66 582.5 67 612.0 68 588.5 69 565.0 70 512.5 71 460.0 72 422.5 73 385.0 74 316.0 75 218.0 76 189.0 77 152.0 78 115.0 79 86.5 80 58.0 81 47.0 82 36.0 83 25.5 84 15.0 85 9.0 86 3.0 87 3.5 88 4.0 89 2.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1919967663702506 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 9896.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.2798640919256 #Duplication Level Percentage of deduplicated Percentage of total 1 97.87918387436328 95.21673704726656 2 1.90771331187526 3.7116418341116515 3 0.1256050219414587 0.36656518391185305 4 0.050017679057416546 0.19462852083596138 5 0.012494634811076169 0.06077381881498661 6 0.012492920979672863 0.07291857930082454 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0124925569718396 0.37673501575817014 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 31 0.31325788197251414 RNA PCR Primer, Index 45 (95% over 21bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.02021018593371059 29 0.0 0.0 0.060630557801131774 30 0.0 0.0 0.09094583670169766 31 0.0 0.0 0.14147130153597412 32 0.0 0.0 0.15157639450282942 33 0.0 0.0 0.2021018593371059 34 0.0 0.0 0.2526273241713824 35 0.0 0.0 0.28294260307194824 36 0.0 0.0 0.4143088116410671 37 0.0 0.0 0.5355699272433306 38 0.0 0.0 0.6669361358124495 39 0.0 0.0 0.9195634599838318 >>END_MODULE >>Kmer Content pass >>END_MODULE