FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_85.3410000000b25c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_85.3410000000b25c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51246
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC3290.6420013269328337No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA1180.23026187409749052No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA760.14830425789329899No Hit
GAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACG650.12683916793505834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.12293642430628732No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC540.10537407797681769RNA PCR Primer, Index 37 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT530.1034227061624322RNA PCR Primer, Index 44 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT1000.031.49863212
AGTACTT1100.028.6351213
GAGACCC607.99635E-626.24886124
GTACTTT1200.026.24886114
CGTATGC551.3438326E-424.5443941
CCCTAGA551.3438326E-424.5443928
GACCCTA551.3438326E-424.5443926
TCTCGTA551.3438326E-424.5443938
ATGCCGT551.3438326E-424.5443944
TACTTTT1301.8189894E-1224.2297215
CTCGTAT602.4238524E-422.49902339
CCTAGAA602.4238524E-422.49902329
TATGCCG602.4238524E-422.49902343
TATCTCG602.4238524E-422.49902336
GTATGCC602.4238524E-422.49902342
AGACCCT602.4238524E-422.49902325
TCGTATG602.4238524E-422.49902340
ACTTTTT1405.456968E-1222.49902316
CGAGACC754.4948527E-520.99908823
CTAGAAT654.163747E-420.7683330
Produced by FastQC (version 0.11.2)