FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_84.3410000000b24f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_84.3410000000b24f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC64.10958904109589RNA PCR Primer, Index 39 (95% over 21bp)
AATATGTACAAATGCACACTGAGTGTTGTAGGATGTTGCTGTCTCTTATAC10.684931506849315No Hit
ACCTGATCGTTCAGGCCACGCATCGCTGGATCGGCCTGTTTCCCGCCGTGC10.684931506849315No Hit
GTACGGAGATGAAGTGATAAAGTAGAATAGAAGAGATGAGATATTTGCTGT10.684931506849315No Hit
CAACTAACTCTTCTTAATTCAAGTTCCTAATGGCTGTCTCTTATACACATC10.684931506849315No Hit
GTATCAACGCAGAGTACGGGGCATCCGGCGGGCAAGCTGAGCGGCGGGCAG10.684931506849315No Hit
GGTTCTTCTAATGAGTCATCAAGCACAGAATGAAATAGCAGTCAAGTATTA10.684931506849315No Hit
CTTCTAGAATATCAGTTAATGCTCTTAACCATTGTACCGCTGTCTCTTTAC10.684931506849315No Hit
CCCTTCTTTGCTTTATTTATGGGACTATTTGCCTATTTACCGAACTGGCTT10.684931506849315No Hit
TGATAATGGCGATTCCTTCCGATACTTCTTGAAATCTATCCTCCTCATATG10.684931506849315No Hit
TTTGGTTTCTGGGCTAATATCCACTTATCAGTGAGTGCATATCAAGTGACT10.684931506849315No Hit
GTCACAGATAGAGGAAGGATGAGGAATTGTTACTATGTAGTCACAGATAAG10.684931506849315No Hit
ACTTCATACTCATTGAAGGTAAAAATCCACCAAGATGACGTCCCAATTCTG10.684931506849315No Hit
GTTAAAAGGTCAACAAAATGCTGAGAATACCTGCCCTGTCTCTTATACACA10.684931506849315No Hit
GTTTCAAATGTTGTATGGAAGTGAGAGGAACGAAAGTGAAGGAGAACGGCT10.684931506849315No Hit
GGATGTGAGTTTTGAATCAGAAGAACATGCTTTGATTGCTTATACATCATT10.684931506849315No Hit
ACTAAGAAAACCAAGATTTTATCCTCTTGATATCCAACAAGGAGTAATCAT10.684931506849315No Hit
GTTAAACAGACAACTCTTAGAAGGGAAATCACTACAAATCTTCACAACCTG10.684931506849315No Hit
TCATACTAGTAGGTTCATCATCATAAACCGGAACAACAATATCATTTCTGT10.684931506849315No Hit
GTGTAAGCACCACCTGCCTTGACCTTGCCACAATCCTTGCAACCCCAGATA10.684931506849315No Hit
TACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGGCTGTCTCTTATAC10.684931506849315No Hit
CTCTTATAGATGAAAAAAATTCGATAGACAAAAACCACCTCAGAACTATCA10.684931506849315No Hit
GAGAAATACACACTTTAGGACGTGAAATATGGTGAGGAAAACTGAAAAAGG10.684931506849315No Hit
AATCTATAACCGGTTTTTCAGAAGATTGGTTGCTTTCTTACTACTACTTCT10.684931506849315No Hit
ACAAGAACTTCGTAAAACATTGCATCTTACGCTTTACTGTATCCATGCTTG10.684931506849315No Hit
CCATTCTATTCCATTCCATTGCAATCGAGTTGAATCCATTGCATTTCATTC10.684931506849315No Hit
GCTAGAGAGAGCTCGAAGACGCAATGGCGAGAATCAAGGTTCATGAGCTGA10.684931506849315No Hit
CAACACGAGAGGGAATGGCATGGAATGGAATGGAATGGAACGGAATGGAAT10.684931506849315No Hit
GGGCCATGATTGGTCAAGTTGCAGGTGGTGGAAGAACAGAGAAGCCGATGC10.684931506849315No Hit
CCATTCCATTCCATTCCATTCCATTCCATTCCGTTCCGTTCCATTCCATTC10.684931506849315No Hit
GTCCTTGAGGGTTGTATGGAAGTCCAGTCAGGTGGGTAACATCCATCTGAC10.684931506849315No Hit
GCCGATGAAGGACGTGGGAGGCTGCGATATGCCTCGGGGAGCTGCCAACCG10.684931506849315No Hit
GCTTCAGTTTGTTCCCAGAATTTGTCTTAGTTTCCAGATATTGCTGTTGTT10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC10.684931506849315No Hit
GTCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGC10.684931506849315No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAG10.684931506849315No Hit
GGACCACTGTTGACAATGGCGTCAATGATGACCTGAATTGGGTTCAAGTCA10.684931506849315No Hit
TTCTAACTCGCAAACTCCACCAAATTTCAAGAACAAACAAACTATATGACT10.684931506849315No Hit
CCTTATGATATCCTGAAATTCTTGCTTCGCTTTACCAATCCTCTCTCGGTC10.684931506849315No Hit
AGATAGATGTGTTCTTCCTAAACTCACGCACAGCGTTCCTCCCGTGCACAC10.684931506849315No Hit
ACCCATATGTTACTGGAACTTCAATTGTTGCAATCAAGTACCTGTCTCTTA10.684931506849315No Hit
ACTTAGAACACAGGATGTCAGCGCCATCTTGTGACGGCGAATGTGGGGGCG10.684931506849315No Hit
CATAAGAGATCCACGTACTTAGATCCACATATAACGAGCAAGTAAACACAT10.684931506849315No Hit
TCAGTACTTCTCTCAATCACAATCTTGTAGTACAGGAACTGTCTCTTATAC10.684931506849315No Hit
ATTGAAAATCCATACACTATATTCTGATCTGTTTCCTTTCTGTCTCTTATA10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGGATCTCGTATGCCGTCT10.684931506849315Illumina PCR Primer Index 2 (95% over 21bp)
ATTGATGAGTGATCACACTATCAGAAATAGGATTGTACAGTAACTGTCTCT10.684931506849315No Hit
GTTCATATCGATCTATTATGCGCCTCTGCATTTAGCATTGGGTAGACCTCA10.684931506849315No Hit
GGTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAATACTAA10.684931506849315Illumina PCR Primer Index 5 (96% over 28bp)
TATCAACGCAGAGTACGGGGGAAAAAAATGAGTTAAACTTATTCAGGCTTA10.684931506849315No Hit
GAAGTATTTAGGCAGCTAAGTGGTCTTAGCAAATTCACACAAGATTCCACG10.684931506849315No Hit
GTCTATACATCCCTTGAACTTATCTAGCGAAAGTTGTAGTGAACAAAATGG10.684931506849315No Hit
GTCTTTCAATACTAAATAGCAAGCAGAATATGAAGGATAAAAGGGGATGTC10.684931506849315No Hit
GTGAAAAGAAAACTGCTATGTTTTTCATTTCAATCCTAAGGTGTCACTCTG10.684931506849315No Hit
GACAAAGAACACTTACTGTAAGAACAAGGAATGCAAAAAGCTGTCTCTTAT10.684931506849315No Hit
CATTAGGCCCGCGTGTGCTTCTACCAGCCATTCCACCTCCACGTGCCATGC10.684931506849315No Hit
GAAATAGGTGATTTCTTAACAGATTCCTGTGCTTGTGATGCGTATTTGCTG10.684931506849315No Hit
GGTATTCCCAAACTTCGGCTCATCTTTCTCTCTACAAAACCCTAATTGAAA10.684931506849315No Hit
TTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGC10.684931506849315No Hit
CTTATACAAATCTCCGAGCCCACGAGACTGTTATACATCTCGTATACCGTC10.684931506849315No Hit
TCCCTTAAGATTACAAAACCATGTTTTCATTGTGCCTTCTCTCTCTGTCTC10.684931506849315No Hit
GCTAGACTCAGATCTGAGACTAGATGTTTCAGATAGCAATTACTGGTGTAA10.684931506849315No Hit
CCTTTCCATTCCATTCCATTCCTTTCCATTCCATTCCATTCCATTCCATTA10.684931506849315No Hit
GAGTCCGACGAAGAAGACTATGGAGGTGATTTCGTTTTGTTCGATGAAGAG10.684931506849315No Hit
AAACGGAGAAGATTGGCCTACTCTTGATGATATGATATGTCAGAACCCTGT10.684931506849315No Hit
GCATATACATTCACATATACACAGGCATGTATACACACCTGTCTCTTACAC10.684931506849315No Hit
TTTTAGTACAGAATCAGAAAGCATATTGTTGTGACTGTCTCTTATACACAT10.684931506849315No Hit
TCTTTGTAGTAAATGGAACCTTCCATGGAATCAAAGTAAACACCCATATGT10.684931506849315No Hit
ATACAATGTAGTCAACGAGTCCACTGAAGAAAGACTTTGAAGATAAGTCTT10.684931506849315No Hit
CCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGT10.684931506849315No Hit
TCAGTTCCCTGAGTTCCCTCGTCAAATTGACGTACCCGCAAGCGGGTGGGT10.684931506849315No Hit
ACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTAACGGGTGACGGAGA10.684931506849315No Hit
ATCACCCTCTATGGCTACTCTTTCCAAAGTATTCTATTAAACATCTGTCTC10.684931506849315No Hit
GCTTGAATCTGTCGCTTCGCATCTTTCTGCTCACCATGATAACTTACGTTA10.684931506849315No Hit
ACTCTCACCTTCTGGTCTTTGTTTTCCCTTCAATAATTTCAGCAATTATGT10.684931506849315No Hit
TAATCATACTTCCTAGAAAATCCGCTACGATTATGTTTATGCAACCCGTAC10.684931506849315No Hit
CGTTGGTGCTGGTGGTGGAGGTGGTGCTCCGGTTGCAGCTGCTGCTCCAGC10.684931506849315No Hit
ACGCAGAGTACGGGGACAAAGTAAACAAGCATACACAAGGAAGTAGGACTG10.684931506849315No Hit
ATGCATATCTTAGGGTTTTATTGCTGTAAAGAGACACTATGATATGCCTGT10.684931506849315No Hit
ATCTATAACACACACACACACACCATGATCAAATGTACACCCTGTCTCTTA10.684931506849315No Hit
CGTTTTCACTTATTCGGGAAATTGAAGAGGTTCGTAAAGACTTGGAATCAT10.684931506849315No Hit
GGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCTGTCTCTTATACAA10.684931506849315No Hit
CTGTTGGAGTGCACGTCCAAGCAGTAAGGTTGAGTGTTAGGCCTGTCTCTT10.684931506849315No Hit
GAACTAGGAATCATAGTCTGTCTTTCATAGGTTCATAAGGAATAAGTTAGG10.684931506849315No Hit
CCAGGAATTGACTTGATAATGTCAGAATCACCAGGATCAACAATGCTCAGA10.684931506849315No Hit
TCACTAAGTAATCAGAAAGATTTATGATCACTTCTAGGTTTGATTCCGAAT10.684931506849315No Hit
TCTCTATGTTTTGTGTTGAACGTTTAGCATTATCTCTATTTGGGATCTGTC10.684931506849315No Hit
ATATGTCATTCTGTAAAAGGCTTGCTTTTCAAGAAAGACCTGAATTAGATC10.684931506849315No Hit
GCTCACTGACAGCTATGAGCATAAAACATAAGGTTTACTTCCTGTCTCTTA10.684931506849315No Hit
GAGAAACAAGTGAGAAGAAAAAACAGTTACGGTTCGATAACTCTTCAATCC10.684931506849315No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGGCCCCGGTTT10.684931506849315No Hit
AAACATACCAACCAACATAGCCGTAGTTTGGCATCTGTCTCTTATACACAT10.684931506849315No Hit
CCCACAAGCTTACTGAAGTCAACCAAACTCCGAATGCCGGTATGCCAAGCG10.684931506849315No Hit
CCACTGGCAGGCGATCGTGTTTTTCACAGGATTTATCGTTACTCATGTCAG10.684931506849315No Hit
GCTGGGCCGTGACGGTCGGAGCCGTCCCTGTCTCTTATAACATCTCCGAGC10.684931506849315No Hit
CGACTTGTGCTCGGAGATTCATGAATGCTAGACCGAGGTGTTGTACTGTTG10.684931506849315No Hit
GTTCTGAGGGCTTCCACAACGGTATAATAAAGTCTTGAACACTCTTGAATA10.684931506849315No Hit
TCATTGCAATTATCCCTATTGTCAATCACAGACCCTGTCTCTTATACACAT10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.684931506849315No Hit
CATTAATTTCAAAACATGAAAGGCACATCATGGTACATCTGTCTCTTATAC10.684931506849315No Hit
CTGTGCTGTGCTGTGCTGTCTGTGCTTTGCTGACTATGCTGTGCTGTGCTG10.684931506849315No Hit
ACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCCTGTCTCTT10.684931506849315No Hit
GTATTAAAGGGAACCTCATCCTCATCACCTTCATCGTCACCGTAGTGTGTT10.684931506849315No Hit
GAGCCATAGCGACGCGCGAGGAAAGCTCGTATGTGCTTAGCAGGAGAAGGA10.684931506849315No Hit
GTGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGGTG10.684931506849315No Hit
GAAGAGAAGAGCTTGACAAGATCATCAGTAGACATATCTGTCTCTTATACA10.684931506849315No Hit
CCATTAATTAACTAGTTGCTGAGTCCTCTATTACAGAGTACAAAGCCTAAG10.684931506849315No Hit
GTATAAGTTTTTAAAAAGCAACTCTTCTGCTGATTTTTTGTTGGTCTTCAC10.684931506849315No Hit
ACATTCGTCAGTGTTACTCAAATGATATTCCTTTTGTACCTGTCTCTTATA10.684931506849315No Hit
TTTGGATTGTTCTGTTTTTTGGGACCCAAAGAGAATTTGCAGTCAAGTTTT10.684931506849315No Hit
GTCTATTCATGTTTGTATCTTCTTCATTGGGTTGGTTGAGGGAAATGGTTT10.684931506849315No Hit
CTATAAGACACTTCTTCTTACCCTTTCGTTTTTGTTTCAGACATATCTGTT10.684931506849315No Hit
CAGCAATGTGATCTCAAAGGGTGAGTCATCGAGCGCTGCAGTGGTTCAGGA10.684931506849315No Hit
CTAGATAACTATGTCTAGTGTGTTGTCTCTATGGTAATTGATCAAAAGTAC10.684931506849315No Hit
GTTCATAACAGATTCAGAGAGGAAAACACGTCGAAATCTCCAGATAGTGGT10.684931506849315No Hit
GTTTCTTATCAATCTCAGTTTCAAGAACGTTGGTTTCCTCTTAGATCTGTC10.684931506849315No Hit
CTCATTAGGTTATCAGTTCTTTGAAGAAAGGAATGGTATTTGATCTGTCTC10.684931506849315No Hit
GAGGCACACACAGCCATGACTGAATATGACAGGACTGAGAAGGATGTGACT10.684931506849315No Hit
TAGTAACTATATCTGATCAGAAGCAATGGAGAAAGGAAAAAAATCTATAGC10.684931506849315No Hit
ATAATTAAGGATGGCATGTTCATATTCCTTCTGTCTCTTATACACATCTCC10.684931506849315No Hit
TATCAACGCAGAGTACGGGTGGATAACAGGTTAAGATCTGTCTCTTATACA10.684931506849315No Hit
GATCGGGGCTCAAGCGATGTACCGAAGCTGCGGATTGAAATTTGCTCTGCA10.684931506849315No Hit
GGGTAGAGTACCTTTGCTTCTAATTTTGGAATAGTTTGTGAAGAACTGGAA10.684931506849315No Hit
CAATTTAAGGGAATTTTAGATACAGGAGCAGACAAAAGTATTGTTTCTTCC10.684931506849315No Hit
AGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCA10.684931506849315No Hit
ATGGGCAATGTATCAACTGTCTCTTTCTCTTATGGAACTTTATTTTAGTGC10.684931506849315No Hit
TCTTCAAGCTGTTTTCCGGCGAAGATCAACCTCTGCTGGTCCGGAGGAATT10.684931506849315No Hit
GATCTGTTTCATACTAAAACCAAAACTCATGTTTGTTCACTCCAAACACAA10.684931506849315No Hit
CTTGTAGGTCATCGAGCCGTCCAGGTTGAACGTGCTGCTGTTGCCCAGGAA10.684931506849315No Hit
GTGCTCTTGAGACTGTGGGTATTTTCTTGGGTACACTTCGAAGTTAGTTTT10.684931506849315No Hit
CAGTGTGAATACCCACACATGAACAACAAACCCTACAGTTACCTCTTACCA10.684931506849315No Hit
GTTCTGCAAACATGTATTATTCAGGCAGCACAAATACTGTCTCTTAACAAT10.684931506849315No Hit
TTATGAGACTCCCTTTGACTTGGTTAGTAATAAAGATAGAACGTATGACTC10.684931506849315No Hit
CTCTAGTATCCATACGTAAATACATAGCGTATGAACTGTCTCTTATACACA10.684931506849315No Hit
ATGCTTCTCCATGAAGACGAGGTTTAATTGATTGTGAGATTTTTCCTTGTA10.684931506849315No Hit
ACCTTGGACTGTTTTGATTTTATACAACTCACATTTGGAAATACAAGTCTC10.684931506849315No Hit
CCTTCAGGTACTGAGATGTTTCAGTTCCCTGAGTTCCCTCGTCAAATTGAC10.684931506849315No Hit
GATTAAGACTGGTCGTGACCAAAAAATAAGAAGCAGCTTCTGATTTCTTCT10.684931506849315No Hit
ACGTTGGAGAGCGTGGCCAAAGAAGGTGACAGCCCTGTAGGCGAAAACAAC10.684931506849315No Hit
TCATTATCCAGAACCTAGTAAACTATGAGTTGTAATTTTAAGGGCCTTGTT10.684931506849315No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)