FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_83.3410000000b232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_83.3410000000b232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326863
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC19270.5895436314296816No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA13930.42617243309888236No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT12350.3778341384616796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.33255522956100875No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10570.3233770723514133No Hit
GTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTG9580.2930891535597483No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC9210.2817694263345806No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACG8350.2554587090004069No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTT7210.22058171160394416No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT6630.20283727433205961No Hit
TATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGA6410.1961066257116896No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGA6110.18692846850209416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.1844809599128687No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCG5890.18019781988172415No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTG5650.1728552941140478No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC5640.1725493555403946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.1691840312302096No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA5460.16704246121463734No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA5380.16459495262541188No Hit
GAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT5350.16367713690445232No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATT5130.15694648828408234No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGAC4990.15266334825293776No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAG4570.13981392815950414No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4500.13767235814393186No Hit
GGTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCT4500.13767235814393186No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC4500.13767235814393186No Hit
GTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATAT4190.12818826236068323No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGG4020.12298730660857914No Hit
TCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCT3940.12053979801935367No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC3930.12023385944570049No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAAC3840.11748041228282186No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGG3840.11748041228282186No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCC3730.11411508797263686No Hit
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTT3520.10769037792592003No Hit
ACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTA3520.10769037792592003No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGC3330.10187754502650959No Hit
GATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGT3320.1015716064528564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.038.302343
TGCCGTC2450.037.65741345
CTCGTAT2400.037.498639
ATGCCGT2450.036.7389444
CCCACGA2450.036.7333219
ACTAAGC2500.035.99865327
TCGTATG2550.035.29279740
ACGAGAC2600.034.6140922
CACGAGA2700.034.1653921
TGATCTC2700.034.1653935
GTATGCC2650.033.96099542
TCTCGTA2650.033.96099538
CCGAGCC2650.033.96099514
CTCCGAG2700.033.3320912
GAGACTA2700.033.3320924
AGCATGA2700.033.3320931
GATCTCG2700.033.3320936
CGTATGC2750.032.72604841
CGAGACT2750.032.72604823
ATCTCGT2850.032.3672137
Produced by FastQC (version 0.11.2)