FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_81.3410000000b218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_81.3410000000b218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972406
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC23860.24537076077276362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3200.035.15528545
ACGAGAC3350.033.5811722
GACTCGC3250.033.2298626
CTCCGAG3550.032.95684412
CGTATGC3350.032.90954641
CGCTATG3350.032.90954630
CGAGACT3450.032.60780323
TATGCCG3500.032.14197543
ATGCCGT3500.032.14197544
ACTCGCT3400.031.76383627
GCCCACG3550.031.68927218
CCGAGCC3650.031.43749214
TCGCTAT3450.031.3034929
CGAGCCC3600.031.24914415
TCCGAGC3650.030.82107213
CACGAGA3650.030.82107221
CCACGAG3650.030.82107220
CTCGCTA3550.030.42170128
ATCTCGT3650.030.2046537
AGACTCG3600.029.99917825
Produced by FastQC (version 0.11.2)