FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_80.3410000000b20b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_80.3410000000b20b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716322
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC125801.7561934437306126RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT41650.5814424239378381No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC36670.5119206167058948No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA13550.18916074056080925No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA13470.18804392438037643No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG12830.17910939493691386No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG7880.11000639377263298No Hit
GTCATAAGACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTG7720.10777276141176732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13150.042.945745
CTCCTCG250.002106836835.9991049
ATGCCGT16900.033.41633244
TATGCCG18550.030.44398943
CATCTCG18650.030.16011236
TCGTATG18750.030.11925340
CTCGTAT18800.030.03914639
GGCCGTA300.00514660429.9992547
CGTATGC18900.029.8802141
ACGAGAC19000.029.84136422
CGAGACT19050.029.76304423
GTTATAC19050.029.76304430
ATCTCGT18950.029.68264237
CACGAGA19200.029.53051621
CCACGAG19350.029.301620
TCTCGTA19650.028.62524438
ACTGTTA20100.028.20825627
TGTTATA20200.028.17999329
GTATGCC20700.027.28193342
GACTGTT21250.026.6816926