Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l01n01 kb2_4_80.3410000000b20b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 716322 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 12580 | 1.7561934437306126 | RNA PCR Primer, Index 39 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 4165 | 0.5814424239378381 | No Hit |
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC | 3667 | 0.5119206167058948 | No Hit |
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA | 1355 | 0.18916074056080925 | No Hit |
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA | 1347 | 0.18804392438037643 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 1283 | 0.17910939493691386 | No Hit |
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG | 788 | 0.11000639377263298 | No Hit |
GTCATAAGACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTG | 772 | 0.10777276141176732 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1315 | 0.0 | 42.9457 | 45 |
CTCCTCG | 25 | 0.0021068368 | 35.999104 | 9 |
ATGCCGT | 1690 | 0.0 | 33.416332 | 44 |
TATGCCG | 1855 | 0.0 | 30.443989 | 43 |
CATCTCG | 1865 | 0.0 | 30.160112 | 36 |
TCGTATG | 1875 | 0.0 | 30.119253 | 40 |
CTCGTAT | 1880 | 0.0 | 30.039146 | 39 |
GGCCGTA | 30 | 0.005146604 | 29.999254 | 7 |
CGTATGC | 1890 | 0.0 | 29.88021 | 41 |
ACGAGAC | 1900 | 0.0 | 29.841364 | 22 |
CGAGACT | 1905 | 0.0 | 29.763044 | 23 |
GTTATAC | 1905 | 0.0 | 29.763044 | 30 |
ATCTCGT | 1895 | 0.0 | 29.682642 | 37 |
CACGAGA | 1920 | 0.0 | 29.530516 | 21 |
CCACGAG | 1935 | 0.0 | 29.3016 | 20 |
TCTCGTA | 1965 | 0.0 | 28.625244 | 38 |
ACTGTTA | 2010 | 0.0 | 28.208256 | 27 |
TGTTATA | 2020 | 0.0 | 28.179993 | 29 |
GTATGCC | 2070 | 0.0 | 27.281933 | 42 |
GACTGTT | 2125 | 0.0 | 26.68169 | 26 |