FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_8.3410000000ad04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_8.3410000000ad04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATTATACTCTACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC12.941176470588235No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC12.941176470588235RNA PCR Primer, Index 39 (95% over 21bp)
GAGCCCTACTATGTAATGTTTTCAAGGATGCTATCTGCTGTCTCTTATACA12.941176470588235No Hit
ATATAAGACCTTTCACTTACAAGTGAAGAGGAATACCATTAGACCTGTCTC12.941176470588235No Hit
TGCAAAAACTTTGTTAGAAGACTTGTCCGTTCTAAACTTTGAAGTAACTGG12.941176470588235No Hit
GTATACACTCTTTACATCCTCATATTCATCTGCTGTCTCTTATACAACATC12.941176470588235No Hit
AAATATGGGGATACCATTCTCCAAGGCTAAGTATAGTATATCTAGCGATAG12.941176470588235No Hit
ACTCAACAGGGCATGAGTTTGCCACAAGGTTGGGGAATGTGTACACCTGTC12.941176470588235No Hit
GTATTGTATGCTAAGTTCAAAGATTCTATCAATCTTGAAGAAGACTAGTTA12.941176470588235No Hit
TCTCAACTTAGTATTATACCCAACAGTACCTAACCTGTCTCTTATACACAT12.941176470588235No Hit
ATACAACACATTACTACACTTCAAGAGGACTTGAAGTCTGTCTCTTATACA12.941176470588235No Hit
GTGCAGAGATTGATAAATCAACTTGTTATTATCATCTCAGCAAACTTGATC12.941176470588235No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCTTTGTGT12.941176470588235Illumina PCR Primer Index 5 (96% over 28bp)
AAACATCATAAGATTTCTCGAAGATACTACACAGTAGCCATACATAAAACC12.941176470588235No Hit
TATCAACGCAGAGTACGGGAAGAAATGCTTCCAGTGTATACAACTGTCTCT12.941176470588235No Hit
ACCTAAAACACGGAGAATTTGATAAAGGACGATCGAATATGAGTGATTTTG12.941176470588235No Hit
CATAGAGAAAGAGTGATGAAATATCCAAACACACGATAAGTCTTAGAAGGC12.941176470588235No Hit
GGAGTATTCTGGTTGCGGAGAAGCCGTGCTGTCAGGCATGGTGGAGCTGTC12.941176470588235No Hit
AAATTATCCGGAAACTGCATAATTGAAAACCATAATAACTGTCTCTTATAC12.941176470588235No Hit
CTCTTTTGCTTTCTTTGATTGGATTAGTGGATTATGCATTTGGAGCTGTCT12.941176470588235No Hit
AAAAAGATCCCATCAAAAGAATTATCATCAAGCTTATCAAGAACATCCTGT12.941176470588235No Hit
GTTGTCAACTCTGTAAAGTCCCTCTACTTCAATAGGTATGGTAACTGTCTC12.941176470588235No Hit
GAGGAAGATGAAGATGATGAAGATGAAGAAGAAGAAGAAGATAACTGAATT12.941176470588235No Hit
GTTTATCGGAGAGGGAGGGGCTTTATTAGATAAAAGCCAAGTTGTTGAACT12.941176470588235No Hit
ATATAATCCACAATTGCTTACGCTTTTTGCTTCAATTCATATACAACGCTC12.941176470588235No Hit
TCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTACC12.941176470588235No Hit
ACTTACCAATCATGTCTAGCAGATCCTTTGTGACCTCTTGGCTAGAACCTG12.941176470588235No Hit
TATTTGCTTTGTTGAGTCATTACCATCTACTGAGACTTTCAAGTTTACGGC12.941176470588235No Hit
CTCGTGGAGTGGACTCCCGTGGTTCCATACCGCGACCACCCGGTGACCGGG12.941176470588235No Hit
GCATTACATTGAACAAGAACGCAGTATTTGGAGACAGTAGTGCTCTTGCTC12.941176470588235No Hit
GGTCAAGATAGTAAGGGTCCACGGTGGATGCCCTGGCACTGGAGCCTGTCT12.941176470588235No Hit
GATGCTGGTTGTTTAATAAAAGTACCTTCACTGGAAGATTCTCTACACGAA12.941176470588235No Hit
GGTTATGTGGGGTTACCCTTCAGTGAATTCATAGCTGTCTGTCTCTTATAC12.941176470588235No Hit
ATATAGTGCATCGGCTCAAGATATGGGGTTGGTATTCAAACAAGGATTTAG12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)