FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_79.3410000000b1fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_79.3410000000b1fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213380
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA19900.16400468113863753No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT18250.15040630305427813No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA15540.12807199723087576No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC12690.10458388963061861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG1900.035.52511227
CTACCGC1900.035.52511228
GCCCACG2050.034.0232418
TATGCCG2150.032.44209743
CCCACGA2150.032.4407619
CCGAGCC2150.032.4407614
CGAGCCC2150.032.4407615
CGTATGC2200.031.7034741
GACTACC2200.031.7034726
TACCGCT2150.031.39428529
AGCCCAC2350.029.67984617
TGCCGTC2450.029.3879745
ATGCCGT2400.029.06271444
GTATGCC2550.027.35201542
CCACGAG2550.027.35201520
ACCGTTC507.160375E-526.9990848
TCCGAGC2600.026.82601413
CGCTGAT2650.026.31986232
ACCGCTG2650.025.47083530
CTCCGAG2750.025.36277812
Produced by FastQC (version 0.11.2)