FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_78.3410000000b1e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_78.3410000000b1e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494042
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC11120.22508207804194783RNA PCR Primer, Index 20 (95% over 21bp)
GATATGTACTCACTGATAAGTGGATATTAGCTCAGAAACCTAGAATACCCA9000.18217074661668442No Hit
CTATAACTCTTTCCATGGGATTTTGTTTCCTATTCTAGGGAGGAATGAAGT6330.12812675845373472No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6280.12711469875030867No Hit
ATTCTAGGGAGGAATGAAGTATCCACATGTTGTTGTTCCTTCTTGATTTTC5870.11881580918221527No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4990.10100355840191724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1500.034.49829539
CCGAGCC1500.034.49829514
CGAGCCC1550.033.38544515
CGTATGC1600.032.3421541
TCGTATG1600.032.3421540
CATCTCG1650.031.36208336
CACGAGA1650.031.36208321
ACGAGAC1650.031.36208322
CTCCGAG1700.030.4396712
TGCCGTC1700.030.4396745
ATGAGCG300.00514569629.99851618
GTATGCC1800.029.99851642
GGACCGC453.4880355E-529.99851644
TCCGAGC1750.029.56996713
CCCACGA1750.029.56996719
ACCGATC702.8805516E-828.92714113
CGAGACC1800.028.74857723
GCCCACG1850.027.97158818
CCACGAG2050.027.43766620
CAGTTCG1400.027.32007813
Produced by FastQC (version 0.11.2)