FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_77.3410000000b1d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_77.3410000000b1d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313562
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC8610.27458684406911554No Hit
GTACTAAGTCAGGGGATATGATTGGAGAACATGAAATGCCAACATGTATAG4560.14542578501221448No Hit
CTATAACTCTTTCCATGGGATTTTGTTTCCTATTCTAGGGAGGAATGAAGT4020.12820431047129435No Hit
GACATAGATCATAAGATAACTCGACTTCATGTGTCCTTAGAAAGACCAGAC3770.12023140559123872No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3480.11098283593037421No Hit
CTTTCATACTGAGTTAGCATTGTCATTGACTGTAGTCTCTATACATGTTGG3420.10906933875916087No Hit
ATGAAAGACAGACTATGATTCCTAGTTCTGTTCCTAAGGAAGACATCATAG3360.10715584158794753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC700.041.78497345
GCCTAGT356.2345243E-638.57074411
ACTCTAT301.1328152E-437.529261
GGTACCG301.1381484E-437.49933611
GTGCTAT301.1381484E-437.49933613
CCCCTGA552.73576E-936.81752837
GGGGATG250.00210496135.9993617
GGTTAGA250.00210496135.9993623
TGCCTAG401.5571526E-533.749410
GTACCGT453.483257E-529.9994712
CTAGTTA453.483257E-529.9994713
AGCTGCC453.483257E-529.999477
CATATGG300.005142064329.9994682
GCGATGT300.005142064329.99946823
GATGCCG300.005142064329.99946836
CACGAGA1100.028.63585521
GTAGATT406.1567046E-428.12450227
GCAATCG654.855119E-727.69181419
CCCACGA1150.027.39081819
CTGGTCA1150.027.3908189