FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_76.3410000000b1c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_76.3410000000b1c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901467
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC36770.4078906937247841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3950.036.45641345
ACGAGAC5150.027.96016522
CCCACGA5200.027.69131919
TCGTATG5200.027.69131940
GCCCACG5150.027.52328918
CGAGACC5150.027.52328923
GAGCCCA5200.027.2586416
CGTATGC5300.027.1688441
CATCTCG5100.026.91080336
CGAGCCC5350.026.4943815
TCCGAGC5500.026.18088213
CCGAGCC5450.026.00824514
TATGCCG5600.025.71479443
ATGCCGT5600.025.71479444
CCACGAG5600.025.7133720
ATCTCGT5650.025.08759937
GAGACCA5600.024.90982624
AGCCCAC5750.024.65129317
CACGAGA6150.024.14547721
CTCCGAG6000.023.99914212
Produced by FastQC (version 0.11.2)