FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_74.3410000000b1ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_74.3410000000b1ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC37.142857142857142RNA PCR Primer, Index 39 (95% over 21bp)
GTACCAAACATGACACATGCTGAACAGTATTCATACTGGCATACGTCTCTG12.380952380952381No Hit
ATCTTATAGAGAATAATCCGCGAGCAACATTTCAGATCAGTAAAATCTGTC12.380952380952381No Hit
GATATACCTTGTTAGAACTCGCATGAGTAAAACTACCAGATAACGTTCGAA12.380952380952381No Hit
GTTCTGATGGTTATTTCTACAGTTGGTTTAGACTTTCTAGTTCTGTTCCTG12.380952380952381No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT12.380952380952381No Hit
ATGGGAGCACCTGCTTTCTCTGGACTTTCTAGCGATGGAGGCATGCAGTGG12.380952380952381No Hit
CTTCTTTTGTTTTCGATTTAGGATTTACCCTCTCTTTTTTTCTGTCTCTTA12.380952380952381No Hit
CTTTAAAGCAGTCACCTTCTTATCCTTACTCTTAAGAGCATCAAGAGTCGC12.380952380952381No Hit
CTTCTATGATTATCCTTCGAAGCTTTTCGTTCTCTACCATTACTTCATCAA12.380952380952381No Hit
TATCAGTGCTTGTTGTTTCATGAAGCAGTTTGTGTATACCTGTCTCTTATA12.380952380952381No Hit
GACCGGCCTGCCCTATCTGTCGGACCGCTCGGATCTCGACCTGCTCTGGCC12.380952380952381No Hit
CTAGTATACTCTACTTGTTCACCTGTGTCGGTTCTGTCTCTTATAACATCT12.380952380952381No Hit
AAGTAAGACTATCAAGAATTCAAGGACATCATAAATGCTGTCTCTTATACA12.380952380952381No Hit
CACTAATATTGTCAACAGAAGAAGATTAGCTACATTCTCTGATCAAAGATT12.380952380952381No Hit
TCATGGAGTTGAACCCTTTATCAAGTGTGGTCAACTCAAGACAGTTAAAGA12.380952380952381No Hit
CTTATACAATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT12.380952380952381RNA PCR Primer, Index 39 (95% over 22bp)
ATATTTAGCTTTAGATGGAGTCTACCACCCACTTTGAGCAGCAATCCCCCC12.380952380952381No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCTGTCTCTTATACACA12.380952380952381No Hit
GGTATACATCATTGACTTTGGTCTTGCTAAGAAGTATAGGGATAGTACCTG12.380952380952381No Hit
GCGCTACATGTACAAAAGAATTTCCCTATATCTTAGGATCGAGTAACTCAT12.380952380952381No Hit
CATCAATAATTTGTGCATAAATGTTTGTGTTTGAACGGTATACGTTCTGTC12.380952380952381No Hit
GACCCGGGCTATGCAGGCTCGCTGCGCCGGCATTTCCCGGCATTGGCCGGC12.380952380952381No Hit
ATCTTAGTCTTAGCATCTTCAGCATCTACAGTTTTACCTTTTTTGTCACCC12.380952380952381No Hit
ACCTAAAGCACCTACTTTACCCATTACCGAAGGCATAGTGATGATTACATC12.380952380952381No Hit
GTTATGCTTATCTTTAGGTGTAAAAGCTGAATGCATACATCAGCAGACATC12.380952380952381No Hit
TGTTAGGAGAATGTGCTGGGAAGCACAATCACAGAGAGTGATAATCCCGTA12.380952380952381No Hit
GGCATGAGTAACGAATTGATAGGTGAGAATCCTATCCTGTCTCTTATACAC12.380952380952381No Hit
GTGGTTGAGAACCAAAACAGTGTAAAGGAAGTTGATGAAGACCATTATATC12.380952380952381No Hit
TTGCAGCCCTACATTTGGGCAATAATAGTGAAATATGCTTGCTCTTAAAAG12.380952380952381No Hit
CATTGATATTCCTAACGAGTGATAAAGGTTTTTACTTTTGAGTTCTCTATC12.380952380952381No Hit
GCCCTCAATCGACGTGATAGGGTACTTTTGGAGGTAGCCCTTGTAGATGGT12.380952380952381No Hit
GCTTATAATATTCGTTTATCTGTCCCCGACCGTAATCTTTTCTTCCTAATA12.380952380952381No Hit
GAACTGGATCTAAGTGTGGATCTTAACGCAGCTAGTGCTGCAGAGGAGGAA12.380952380952381No Hit
TGTATCCTCAATATCAAAATCCTCGAACACAATTAAAACTGTCTCTTATAC12.380952380952381No Hit
CAAGTACACAAGGAAGAAGAAGTAACTCTTCATTCTGTCTCTTATACACAT12.380952380952381No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCCCGTACTCTGAGTTGATACCC12.380952380952381No Hit
CCCTACCACTGAGAAATCAAGATGGCAGTCTACTATGGGGAATTGAGGAAA12.380952380952381No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAAC12.380952380952381No Hit
CTGCAATTATCTCCATGGTAAGGAATTTTAGGAATTCCGTTTCACTCCTGT12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)