##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23V3BCXX l01n01 kb2_4_74.3410000000b1ad.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.404761904761905	0.0	0.0	0.0	0.0	0.0
2	34.404761904761905	0.0	0.0	0.0	0.0	0.0
3	34.833333333333336	0.0	0.0	0.0	0.0	0.0
4	35.57142857142857	0.0	0.0	0.0	0.0	0.0
5	35.54761904761905	0.0	0.0	0.0	0.0	0.0
6	37.23809523809524	0.0	0.0	0.0	0.0	0.0
7	36.404761904761905	0.0	0.0	0.0	0.0	0.0
8	36.57142857142857	0.0	0.0	0.0	0.0	0.0
9	36.42857142857143	0.0	0.0	0.0	0.0	0.0
10	37.19047619047619	0.0	0.0	0.0	0.0	0.0
11	37.857142857142854	0.0	0.0	0.0	0.0	0.0
12	37.42857142857143	0.0	0.0	0.0	0.0	0.0
13	35.76190476190476	0.0	0.0	0.0	0.0	0.0
14	36.76190476190476	0.0	0.0	0.0	0.0	0.0
15	36.04761904761905	0.0	0.0	0.0	0.0	0.0
16	36.30952380952381	0.0	0.0	0.0	0.0	0.0
17	34.95238095238095	0.0	0.0	0.0	0.0	0.0
18	33.833333333333336	0.0	0.0	0.0	0.0	0.0
19	36.38095238095238	0.0	0.0	0.0	0.0	0.0
20	34.642857142857146	0.0	0.0	0.0	0.0	0.0
21	34.714285714285715	0.0	0.0	0.0	0.0	0.0
22	35.19047619047619	0.0	0.0	0.0	0.0	0.0
23	34.904761904761905	0.0	0.0	0.0	0.0	0.0
24	36.404761904761905	0.0	0.0	0.0	0.0	0.0
25	36.285714285714285	0.0	0.0	0.0	0.0	0.0
26	36.07142857142857	0.0	0.0	0.0	0.0	0.0
27	36.26190476190476	0.0	0.0	0.0	0.0	0.0
28	36.88095238095238	0.0	0.0	0.0	0.0	0.0
29	36.166666666666664	0.0	0.0	0.0	0.0	0.0
30	36.523809523809526	0.0	0.0	0.0	0.0	0.0
31	37.142857142857146	0.0	0.0	0.0	0.0	0.0
32	37.0	0.0	0.0	0.0	0.0	0.0
33	37.45238095238095	0.0	0.0	0.0	0.0	0.0
34	38.26190476190476	0.0	0.0	0.0	0.0	0.0
35	36.19047619047619	0.0	0.0	0.0	0.0	0.0
36	36.76190476190476	0.0	0.0	0.0	0.0	0.0
37	35.30952380952381	0.0	0.0	0.0	0.0	0.0
38	35.357142857142854	0.0	0.0	0.0	0.0	0.0
39	36.166666666666664	0.0	0.0	0.0	0.0	0.0
40	35.357142857142854	0.0	0.0	0.0	0.0	0.0
41	35.42857142857143	0.0	0.0	0.0	0.0	0.0
42	35.04761904761905	0.0	0.0	0.0	0.0	0.0
43	34.69047619047619	0.0	0.0	0.0	0.0	0.0
44	34.714285714285715	0.0	0.0	0.0	0.0	0.0
45	34.214285714285715	0.0	0.0	0.0	0.0	0.0
46	35.285714285714285	0.0	0.0	0.0	0.0	0.0
47	36.04761904761905	0.0	0.0	0.0	0.0	0.0
48	35.07142857142857	0.0	0.0	0.0	0.0	0.0
49	35.30952380952381	0.0	0.0	0.0	0.0	0.0
50	34.88095238095238	0.0	0.0	0.0	0.0	0.0
51	34.023809523809526	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2103	1	1.5
2103	2	-1.5
2103	3	1.5
2103	4	1.5
2103	5	-1.5
2103	6	NaN
2103	7	1.0
2103	8	2.5
2103	9	NaN
2103	10	1.0
2103	11	-0.5
2103	12	-0.5
2103	13	-1.0
2103	14	0.5
2103	15	0.0
2103	16	NaN
2103	17	-1.0
2103	18	NaN
2103	19	2.5
2103	20	1.0
2103	21	0.5
2103	22	-1.0
2103	23	0.5
2103	24	1.5
2103	25	3.0
2103	26	0.0
2103	27	-0.5
2103	28	2.0
2103	29	1.5
2103	30	0.5
2103	31	0.5
2103	32	-0.5
2103	33	0.5
2103	34	3.0
2103	35	-0.5
2103	36	3.0
2103	37	-0.5
2103	38	-0.5
2103	39	-1.5
2103	40	NaN
2103	41	1.0
2103	42	-0.5
2103	43	-3.5
2103	44	-0.5
2103	45	0.5
2103	46	-1.0
2103	47	-1.0
2103	48	1.0
2103	49	1.5
2103	50	NaN
2103	51	1.0
2113	1	1.5
2113	2	4.5
2113	3	1.5
2113	4	1.5
2113	5	4.5
2113	6	NaN
2113	7	1.0
2113	8	2.5
2113	9	NaN
2113	10	3.0
2113	11	1.5
2113	12	1.5
2113	13	1.0
2113	14	0.5
2113	15	0.0
2113	16	NaN
2113	17	1.0
2113	18	NaN
2113	19	2.5
2113	20	3.0
2113	21	0.5
2113	22	1.0
2113	23	2.5
2113	24	1.5
2113	25	1.0
2113	26	0.0
2113	27	1.5
2113	28	2.0
2113	29	-0.5
2113	30	0.5
2113	31	0.5
2113	32	1.5
2113	33	0.5
2113	34	1.0
2113	35	1.5
2113	36	1.0
2113	37	-0.5
2113	38	-0.5
2113	39	0.5
2113	40	NaN
2113	41	1.0
2113	42	1.5
2113	43	4.5
2113	44	1.5
2113	45	2.5
2113	46	1.0
2113	47	1.0
2113	48	3.0
2113	49	1.5
2113	50	NaN
2113	51	3.0
1204	1	1.5
1204	2	-1.5
1204	3	-4.5
1204	4	-4.5
1204	5	-1.5
1204	6	NaN
1204	7	-1.0
1204	8	-5.5
1204	9	NaN
1204	10	1.0
1204	11	-0.5
1204	12	-0.5
1204	13	-1.0
1204	14	-1.5
1204	15	0.0
1204	16	NaN
1204	17	-1.0
1204	18	NaN
1204	19	-5.5
1204	20	-5.0
1204	21	-1.5
1204	22	-1.0
1204	23	-5.5
1204	24	-4.5
1204	25	-5.0
1204	26	0.0
1204	27	-0.5
1204	28	-6.0
1204	29	-0.5
1204	30	-1.5
1204	31	-1.5
1204	32	-0.5
1204	33	-1.5
1204	34	-5.0
1204	35	-0.5
1204	36	-5.0
1204	37	-0.5
1204	38	-0.5
1204	39	0.5
1204	40	NaN
1204	41	-1.0
1204	42	-0.5
1204	43	2.5
1204	44	-0.5
1204	45	2.5
1204	46	1.0
1204	47	1.0
1204	48	1.0
1204	49	-4.5
1204	50	NaN
1204	51	-5.0
2209	1	-4.5
2209	2	-1.5
2209	3	1.5
2209	4	1.5
2209	5	-1.5
2209	6	NaN
2209	7	-1.0
2209	8	0.5
2209	9	NaN
2209	10	-5.0
2209	11	-0.5
2209	12	-0.5
2209	13	1.0
2209	14	0.5
2209	15	0.0
2209	16	NaN
2209	17	1.0
2209	18	NaN
2209	19	0.5
2209	20	1.0
2209	21	0.5
2209	22	1.0
2209	23	2.5
2209	24	1.5
2209	25	1.0
2209	26	0.0
2209	27	-0.5
2209	28	2.0
2209	29	-0.5
2209	30	0.5
2209	31	0.5
2209	32	-0.5
2209	33	0.5
2209	34	1.0
2209	35	-0.5
2209	36	1.0
2209	37	1.5
2209	38	1.5
2209	39	0.5
2209	40	NaN
2209	41	-1.0
2209	42	-0.5
2209	43	-3.5
2209	44	-0.5
2209	45	-5.5
2209	46	-1.0
2209	47	-1.0
2209	48	-5.0
2209	49	1.5
2209	50	NaN
2209	51	1.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	0.0
26	0.0
27	1.0
28	0.0
29	0.0
30	1.0
31	2.0
32	4.0
33	5.0
34	2.0
35	0.0
36	6.0
37	5.0
38	5.0
39	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.33333333333333	19.047619047619047	14.285714285714285	33.33333333333333
2	9.523809523809524	23.809523809523807	50.0	16.666666666666664
3	14.285714285714285	16.666666666666664	45.23809523809524	23.809523809523807
4	9.523809523809524	23.809523809523807	33.33333333333333	33.33333333333333
5	11.904761904761903	28.57142857142857	52.38095238095239	7.142857142857142
6	28.57142857142857	50.0	14.285714285714285	7.142857142857142
7	19.047619047619047	26.190476190476193	21.428571428571427	33.33333333333333
8	21.428571428571427	50.0	14.285714285714285	14.285714285714285
9	11.904761904761903	16.666666666666664	28.57142857142857	42.857142857142854
10	7.142857142857142	33.33333333333333	45.23809523809524	14.285714285714285
11	19.047619047619047	23.809523809523807	38.095238095238095	19.047619047619047
12	7.142857142857142	21.428571428571427	47.61904761904761	23.809523809523807
13	19.047619047619047	23.809523809523807	28.57142857142857	28.57142857142857
14	16.666666666666664	26.190476190476193	26.190476190476193	30.952380952380953
15	16.666666666666664	40.476190476190474	19.047619047619047	23.809523809523807
16	21.428571428571427	23.809523809523807	33.33333333333333	21.428571428571427
17	26.190476190476193	33.33333333333333	33.33333333333333	7.142857142857142
18	33.33333333333333	14.285714285714285	30.952380952380953	21.428571428571427
19	23.809523809523807	28.57142857142857	30.952380952380953	16.666666666666664
20	19.047619047619047	28.57142857142857	14.285714285714285	38.095238095238095
21	19.047619047619047	26.190476190476193	33.33333333333333	21.428571428571427
22	38.095238095238095	26.190476190476193	9.523809523809524	26.190476190476193
23	19.047619047619047	28.57142857142857	35.714285714285715	16.666666666666664
24	19.047619047619047	38.095238095238095	23.809523809523807	19.047619047619047
25	26.190476190476193	28.57142857142857	16.666666666666664	28.57142857142857
26	19.047619047619047	28.57142857142857	26.190476190476193	26.190476190476193
27	23.809523809523807	28.57142857142857	26.190476190476193	21.428571428571427
28	16.666666666666664	19.047619047619047	30.952380952380953	33.33333333333333
29	19.047619047619047	19.047619047619047	40.476190476190474	21.428571428571427
30	19.047619047619047	23.809523809523807	28.57142857142857	28.57142857142857
31	23.809523809523807	21.428571428571427	40.476190476190474	14.285714285714285
32	16.666666666666664	28.57142857142857	30.952380952380953	23.809523809523807
33	14.285714285714285	28.57142857142857	42.857142857142854	14.285714285714285
34	16.666666666666664	30.952380952380953	38.095238095238095	14.285714285714285
35	7.142857142857142	45.23809523809524	33.33333333333333	14.285714285714285
36	11.904761904761903	21.428571428571427	40.476190476190474	26.190476190476193
37	23.809523809523807	21.428571428571427	23.809523809523807	30.952380952380953
38	23.809523809523807	19.047619047619047	23.809523809523807	33.33333333333333
39	16.666666666666664	19.047619047619047	38.095238095238095	26.190476190476193
40	33.33333333333333	9.523809523809524	35.714285714285715	21.428571428571427
41	19.047619047619047	19.047619047619047	40.476190476190474	21.428571428571427
42	14.285714285714285	26.190476190476193	33.33333333333333	26.190476190476193
43	16.666666666666664	23.809523809523807	40.476190476190474	19.047619047619047
44	7.142857142857142	30.952380952380953	40.476190476190474	21.428571428571427
45	28.57142857142857	21.428571428571427	40.476190476190474	9.523809523809524
46	19.047619047619047	19.047619047619047	40.476190476190474	21.428571428571427
47	9.523809523809524	30.952380952380953	21.428571428571427	38.095238095238095
48	16.666666666666664	21.428571428571427	26.190476190476193	35.714285714285715
49	26.190476190476193	30.952380952380953	14.285714285714285	28.57142857142857
50	16.666666666666664	23.809523809523807	40.476190476190474	19.047619047619047
51	11.904761904761903	26.190476190476193	19.047619047619047	42.857142857142854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	2.5
31	5.0
32	3.0
33	1.0
34	3.0
35	5.0
36	6.0
37	7.0
38	5.5
39	4.0
40	4.0
41	4.0
42	2.5
43	1.0
44	2.0
45	3.0
46	3.0
47	3.0
48	3.5
49	4.0
50	2.5
51	1.0
52	0.5
53	0.0
54	0.5
55	1.0
56	0.5
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.5
69	1.0
70	1.0
71	1.0
72	1.0
73	1.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	42.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.23809523809523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.5	92.85714285714286
2	0.0	0.0
3	2.5	7.142857142857142
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	3	7.142857142857142	RNA PCR Primer, Index 39 (95% over 21bp)
GTACCAAACATGACACATGCTGAACAGTATTCATACTGGCATACGTCTCTG	1	2.380952380952381	No Hit
ATCTTATAGAGAATAATCCGCGAGCAACATTTCAGATCAGTAAAATCTGTC	1	2.380952380952381	No Hit
GATATACCTTGTTAGAACTCGCATGAGTAAAACTACCAGATAACGTTCGAA	1	2.380952380952381	No Hit
GTTCTGATGGTTATTTCTACAGTTGGTTTAGACTTTCTAGTTCTGTTCCTG	1	2.380952380952381	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT	1	2.380952380952381	No Hit
ATGGGAGCACCTGCTTTCTCTGGACTTTCTAGCGATGGAGGCATGCAGTGG	1	2.380952380952381	No Hit
CTTCTTTTGTTTTCGATTTAGGATTTACCCTCTCTTTTTTTCTGTCTCTTA	1	2.380952380952381	No Hit
CTTTAAAGCAGTCACCTTCTTATCCTTACTCTTAAGAGCATCAAGAGTCGC	1	2.380952380952381	No Hit
CTTCTATGATTATCCTTCGAAGCTTTTCGTTCTCTACCATTACTTCATCAA	1	2.380952380952381	No Hit
TATCAGTGCTTGTTGTTTCATGAAGCAGTTTGTGTATACCTGTCTCTTATA	1	2.380952380952381	No Hit
GACCGGCCTGCCCTATCTGTCGGACCGCTCGGATCTCGACCTGCTCTGGCC	1	2.380952380952381	No Hit
CTAGTATACTCTACTTGTTCACCTGTGTCGGTTCTGTCTCTTATAACATCT	1	2.380952380952381	No Hit
AAGTAAGACTATCAAGAATTCAAGGACATCATAAATGCTGTCTCTTATACA	1	2.380952380952381	No Hit
CACTAATATTGTCAACAGAAGAAGATTAGCTACATTCTCTGATCAAAGATT	1	2.380952380952381	No Hit
TCATGGAGTTGAACCCTTTATCAAGTGTGGTCAACTCAAGACAGTTAAAGA	1	2.380952380952381	No Hit
CTTATACAATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT	1	2.380952380952381	RNA PCR Primer, Index 39 (95% over 22bp)
ATATTTAGCTTTAGATGGAGTCTACCACCCACTTTGAGCAGCAATCCCCCC	1	2.380952380952381	No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCTGTCTCTTATACACA	1	2.380952380952381	No Hit
GGTATACATCATTGACTTTGGTCTTGCTAAGAAGTATAGGGATAGTACCTG	1	2.380952380952381	No Hit
GCGCTACATGTACAAAAGAATTTCCCTATATCTTAGGATCGAGTAACTCAT	1	2.380952380952381	No Hit
CATCAATAATTTGTGCATAAATGTTTGTGTTTGAACGGTATACGTTCTGTC	1	2.380952380952381	No Hit
GACCCGGGCTATGCAGGCTCGCTGCGCCGGCATTTCCCGGCATTGGCCGGC	1	2.380952380952381	No Hit
ATCTTAGTCTTAGCATCTTCAGCATCTACAGTTTTACCTTTTTTGTCACCC	1	2.380952380952381	No Hit
ACCTAAAGCACCTACTTTACCCATTACCGAAGGCATAGTGATGATTACATC	1	2.380952380952381	No Hit
GTTATGCTTATCTTTAGGTGTAAAAGCTGAATGCATACATCAGCAGACATC	1	2.380952380952381	No Hit
TGTTAGGAGAATGTGCTGGGAAGCACAATCACAGAGAGTGATAATCCCGTA	1	2.380952380952381	No Hit
GGCATGAGTAACGAATTGATAGGTGAGAATCCTATCCTGTCTCTTATACAC	1	2.380952380952381	No Hit
GTGGTTGAGAACCAAAACAGTGTAAAGGAAGTTGATGAAGACCATTATATC	1	2.380952380952381	No Hit
TTGCAGCCCTACATTTGGGCAATAATAGTGAAATATGCTTGCTCTTAAAAG	1	2.380952380952381	No Hit
CATTGATATTCCTAACGAGTGATAAAGGTTTTTACTTTTGAGTTCTCTATC	1	2.380952380952381	No Hit
GCCCTCAATCGACGTGATAGGGTACTTTTGGAGGTAGCCCTTGTAGATGGT	1	2.380952380952381	No Hit
GCTTATAATATTCGTTTATCTGTCCCCGACCGTAATCTTTTCTTCCTAATA	1	2.380952380952381	No Hit
GAACTGGATCTAAGTGTGGATCTTAACGCAGCTAGTGCTGCAGAGGAGGAA	1	2.380952380952381	No Hit
TGTATCCTCAATATCAAAATCCTCGAACACAATTAAAACTGTCTCTTATAC	1	2.380952380952381	No Hit
CAAGTACACAAGGAAGAAGAAGTAACTCTTCATTCTGTCTCTTATACACAT	1	2.380952380952381	No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCCCGTACTCTGAGTTGATACCC	1	2.380952380952381	No Hit
CCCTACCACTGAGAAATCAAGATGGCAGTCTACTATGGGGAATTGAGGAAA	1	2.380952380952381	No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAAC	1	2.380952380952381	No Hit
CTGCAATTATCTCCATGGTAAGGAATTTTAGGAATTCCGTTTCACTCCTGT	1	2.380952380952381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
25	0.0	0.0	0.0
26	0.0	0.0	0.0
27	0.0	0.0	0.0
28	0.0	0.0	0.0
29	0.0	0.0	0.0
30	0.0	0.0	0.0
31	0.0	0.0	0.0
32	0.0	0.0	0.0
33	0.0	0.0	0.0
34	0.0	0.0	2.380952380952381
35	0.0	0.0	4.761904761904762
36	0.0	0.0	7.142857142857143
37	0.0	0.0	9.523809523809524
38	0.0	0.0	11.904761904761905
39	0.0	0.0	14.285714285714286
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE