FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_73.3410000000b183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_73.3410000000b183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144511
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.15016157939534014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA300.00510711330.030481
TAGGCCA300.005133131529.99930834
GTTTTAT500.002241833622.5228611
GGTATCA2800.022.522861
GGGACTG500.002255415522.4994835
ACAAACT602.4534404E-422.49948115
GTATCAA5500.021.7038461
TATATGA550.00392068420.4540752
ACTGTGC550.00392068420.4540758
TCTTAGG600.006484488518.7495672
AACGCAG6450.018.4879466
GGGAGAT750.001104021417.99958612
ACGCAGA6800.017.8672357
CGCAGAG6650.017.5935788
ACATCTC1802.0190782E-1017.49959645
ATCTCCG1053.082709E-517.1424645
TATCAAC7050.016.9145032
TCAACGC7350.016.2241154
CAACGCA7400.016.1144925
ATCAACG7400.016.1144923
Produced by FastQC (version 0.11.2)