FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_71.3410000000b169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_71.3410000000b169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC21.2738853503184715No Hit
ATTTGGTTGCGGGGTTGAGTTTTGGATATGTTTGGTTTTGACGCTACTTGG10.6369426751592357No Hit
TTTCTTTGTCTCTTCAACAATCTCCTCTTTCTCACCACTTGTTCCTTCGGT10.6369426751592357No Hit
CCAGGAGACTGTTCAATACCCAGTCGAGCACCCAGAGAAGTTTGAGAAATT10.6369426751592357No Hit
CTCATTTCCTTTTACCTCTTTTTCTGAATTGGGATTCTCTCTTTTTGTGCT10.6369426751592357No Hit
TACCAATACAGAACAATCATTCATCACTTTGATTTGAGCTTAATCTTAGAA10.6369426751592357No Hit
TACTTAGTTCCACCATCTTTTCCTCGCGTTTGGACTCTCGAAGGTCAGGGA10.6369426751592357No Hit
GGAGTGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGG10.6369426751592357No Hit
GACCAAGCCTGTCTTCCAAAAGAAGGCCAAGACAACCAAGAAGGTTGTGCT10.6369426751592357No Hit
GTTATTTTAAGGATTTTTCGAAGGTTATTATCTTGTGCTGCCTGTCTCTTA10.6369426751592357No Hit
CCCCTACCTCGATGATGATGATGAAGTTTGATTATGAAGATGGTGTTCATT10.6369426751592357No Hit
GCTTCAGGTGAGCTCGATTCTGTCCGGCTCCGATGCGACTCCCAGCGCCAC10.6369426751592357No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.6369426751592357No Hit
CTTCCCTGCCTTGTCAGACTTCTTCTTCTTCTTACGAGTGTCCAATGCTGC10.6369426751592357No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCG10.6369426751592357No Hit
GCTACAAGCTGGTGTGGAAGGCCGAACCGAACCCGTTCGACAAGGCCCGCG10.6369426751592357No Hit
GGAACAATTCATGTAACGAGATATTAGAAGCCTCCGAAACAACAGGTACTG10.6369426751592357No Hit
GTGTTAACAAGCATTGCTAAAAATGCTGGAAGGAACAATTGTAAATGTACC10.6369426751592357No Hit
GATTGTAGCTAATTCCGTTCAGTTCTATTTCCTGTCTCTTATACACATCTC10.6369426751592357No Hit
AACCAAGATCGGATATAGCTGGTTTTCCGCGAAATCTATTTCAGTAGAGCG10.6369426751592357No Hit
AGGAAAGAACAGAGGAGATAGGAAAAATCACTTGGTGATGATATAAAGAGC10.6369426751592357No Hit
CAACCAAAGAAACTCTCAAGAACTCTCTCGTTCTTAGGATTCTCTTTGTTG10.6369426751592357No Hit
TCACTGTCTTCTTGTGTCTCAGAAACAGAGCTTTGTTCTCTGTCTTTTTTT10.6369426751592357No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGACTGTCTCT10.6369426751592357No Hit
GGATACATGGCTGTCTGGTGGTGAAGTTTACTGGAAAACAAACTTGCAATT10.6369426751592357No Hit
CAACCCAGTTGAGAATTCCAGCTTCTTTCAGAAGAAAAGCTACAAACTTGC10.6369426751592357No Hit
GAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCC10.6369426751592357No Hit
TTATACGGGAGAGTTCGCATCTGGCTGCAGCGTTTCCTCTGTCTCTTATAC10.6369426751592357No Hit
CCCATATACATTATACTGAATTGTTGGCTATAAGCAACTTAATGTTAAGAA10.6369426751592357No Hit
ATGTTATTGTCAAGAACTCATGGGGACCAAGATGGGGAGAAAAAGGGTTTA10.6369426751592357No Hit
TCTTGTTAAGTTGTTCCATCTCAACAACATCAGCCATTTAGATTGATAGCC10.6369426751592357No Hit
GGATTATTAAGATATGCACTTGGACTGCGGTTTACACCAGCATCCAAGAGC10.6369426751592357No Hit
GATAAAGGAGGGTTTAAAAAAAGAACATAACAACTGGTAAGAAAAACATCT10.6369426751592357No Hit
ATACATGATGACAGCAACAAGAAGCTCGTCTGCGAAAATCCTAATGTGATT10.6369426751592357No Hit
CTCTGAACCTCTTCTTACTTTGGGTCATGGTTTCACCAAGTCACGTGGACT10.6369426751592357No Hit
GTAAGCGATAGTGAAAAAGTACCGTGAGGGAATACATGAAATAGTAGTTTT10.6369426751592357No Hit
CCCCTGGAGCATCCAGGGGCTGGACCACCAACCAAAGAGTACACATTGAGG10.6369426751592357No Hit
ATAGAGGAGGAACCTCTAGTCTAGATATCGATCTACTTGGTGCCAACTGAA10.6369426751592357No Hit
CAATTACAGAAGGAACTATATCTACTTGGTTTAAGCAAGTAGGCGACAGTG10.6369426751592357No Hit
TTGCAGAGTCCTTCAAACTTCTCCTTGAGCTCTTCTTTCTTCTTCTTTTCA10.6369426751592357No Hit
AGTCTAGCCACGAAATGACCTCGACGGAGCCTACGCTGTCTCTTATACACA10.6369426751592357No Hit
GCATTAGTCATCAAGGATGAAAGTGAAGCAAACCTTTCTGCTGCGAGTAGA10.6369426751592357No Hit
GGCTTAAGGTGACCACTTGTGGTGACAAATAAAGTGCATTCCAGAAGAAGA10.6369426751592357No Hit
TCACAGCACCCTTTAGGAATCTTCAGTCAAGGTACGAAGTAACTCGACTGA10.6369426751592357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC10.6369426751592357No Hit
GTTGTTGTCCCAAGTACTGTCTTGCCCAAAATTCTGATCTTAAGTTCCACA10.6369426751592357No Hit
CTTATATCTTTCGTTGGAATCTCATTAAGATCAACCCATTCCCATGTCTCA10.6369426751592357No Hit
CATCAGGCAGCAAGTGGTCGGCAGCGCTAGCTGGACGCTGCCGACGACTCG10.6369426751592357No Hit
TAGCTATACGACTTCTCCTTAGCAATGGCATAAATCTGTCTCTTATACAAT10.6369426751592357No Hit
CTTATACAATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTCT10.6369426751592357No Hit
GTAACACTTCTTTAAGAAGATTACCTTCTTTATACAAATGGCATTGGACAG10.6369426751592357No Hit
GTCCTGGTTCGGTCCTCCAGTGCGTCTTACCGCACCTTCAACCTGCACATG10.6369426751592357No Hit
TCCTTCTTTCTCTCTTCTATCTGTGAACAAGGCACATTAGCTGTCTCTTAT10.6369426751592357No Hit
CTAAAACAATCATCCTTAAGAATAATGGTGATTTCTTTGGTTGCTTTATAA10.6369426751592357No Hit
ACTTAGATTGGACTAATAGTAAGGTAACGTCATTATCCTCCCTTACCAGAC10.6369426751592357No Hit
CATATGTATGACATCTGGCTTATCAATTAGATATGCATCTGTCTCTTATAC10.6369426751592357No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCC10.6369426751592357No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAG10.6369426751592357No Hit
GAACACTATCGATCTAATCTTTTCAGCTCTCTCCATCGTCGTCGTATCTGT10.6369426751592357No Hit
GATTGAGATTGTGTACAACTATGGTCTAGAAGACAACCCATAATAAACCTA10.6369426751592357No Hit
CATCAAAAATATCCTCAAAACCACTAGGATCAGCCCAGAAAGATGTTTTGT10.6369426751592357No Hit
CACTAAAAGTTAGGGTTTACTAGAGGATGTAATCTTTAGGGTTCTTTGACT10.6369426751592357No Hit
CATTAGAGACATAGATGAAAATCTGAGCGTAATCCCTGTCTCTTATCACAT10.6369426751592357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCTT10.6369426751592357No Hit
AGCTAGTTTGTATCAAAGTCATTGTTCCTTCTAAAGATTTCGCACAAAAGC10.6369426751592357No Hit
TCCAAAAACCTCAAGGTACTTCATGAGTTGTCGATAAAAACATAAGTGCTG10.6369426751592357No Hit
GCATAATAATCACACAACAAAAGCGCACTCTCACTCTCTATGACTCTTTAT10.6369426751592357No Hit
CTACTGGCGCTTCAAAAGGCGAAAGTCTCGTCGTAGTTTGGCGACCTTCAG10.6369426751592357No Hit
ATCCGAGGCTGTACCTCAATACATTTCGGGGAGAACTAGCTATCTCCAGGT10.6369426751592357No Hit
GTACTACCACTAGTCACAAACGACAAACAATCAGCCATGTTTAGTATCAAC10.6369426751592357No Hit
AAAAACAAATTTTATCACATTTCTCCTCTGTATTTTCATTTTCTCTCATTT10.6369426751592357No Hit
GAACAATAATAATATGGGGATGTCTAAAAGTATCAAGGTTATCCCTGTCTC10.6369426751592357No Hit
TACCAAGGGTTGTGACCATTTGAGAGATGTCTTTTGCTAAGCAGCTGTCTC10.6369426751592357No Hit
CACGACATGCTTGTTATCTAACCATGAAGTCTTGACGGTACAAATGAAAAA10.6369426751592357No Hit
ATCGGTGGGAGGGAGATTGAAGAACGGTAAACGGAAAATATGAAGACGACG10.6369426751592357No Hit
CTTATACACATCTCCGAGCCCACGGAGACAGGATGTGATCTCGTATGCCGT10.6369426751592357No Hit
GTGTTAGAGAAACCCACAATGGAAGTTTCACAGATACAATTCAACTTCTGG10.6369426751592357No Hit
GATGAGCACACGGGCATGGTGAATAGATTTGGCCAGGCCCAGCTTAAAGAC10.6369426751592357No Hit
GAGTAAATCAGTAACCCATTTTTCCATTGCGTTCATTAATTTGGATCTTTG10.6369426751592357No Hit
CCTTGGAATATTCATCCCGTCCACCTGTGTCGGTTTGCGGTACGGTCTAGT10.6369426751592357No Hit
GTATCAACGCAGAGTACGGGGATATGTTGAGAATGGCTCACACCAATCATG10.6369426751592357No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC10.6369426751592357No Hit
CTATCAGTTCTGAAGGCACGGTAGAGTGGTCGATACCATAACACATAGCCT10.6369426751592357No Hit
GCTTTCCATGTGCTCGGAATATCGATAGGCATAATGTTCTCAGGCTTAAAG10.6369426751592357No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT10.6369426751592357No Hit
ATCTTACGAGCAATTTGGTGCATTTCTTGGAAATGTGAAGGATCTTAACGC10.6369426751592357No Hit
AATTAGAGGTTCTCCTTTTATTCTTTTTTCCTTCCGAGGATAGAATCTGTC10.6369426751592357No Hit
TTCTCATTCTGCCTGGATTCGGTATCATAAGTCATATCGTTTCTGTCTCTT10.6369426751592357No Hit
CACATGAACACAGACATTCCTTGAAGCTTTCTCCATCCTCATATCTCTGTC10.6369426751592357No Hit
GTCATTAGCTGTGGATACGAAGAGAAAACTGAATGGGGAAAAGAAATAGGA10.6369426751592357No Hit
TCTTTCCCACTTTCAATATCACGTAACACCTGAAGAGGGAGATCTGTCTCT10.6369426751592357No Hit
ATCAAGACCAACACCAGATGTATCTTGTTTGTTCGTAACTCTCACATAACC10.6369426751592357No Hit
GACATACACCGGACCGGGCCAGAGATGGTCTTTCCCCCTTGTGGGGCTGGT10.6369426751592357No Hit
GGACTATATTCAAGACGAAGATGGACCTAGAGAATTTGGCAACATATCACT10.6369426751592357No Hit
ACTGTGGATAGCATGCATGATTTAGTATACATCGATGACGGGTGTATCTGT10.6369426751592357No Hit
TATCAACGCAGAGTACGGGAGGTGTGATCGTCATTTTAAGTCACTGTCAGA10.6369426751592357No Hit
CGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTT10.6369426751592357No Hit
GTAGAAATGAGAGAATTGGAGAAAGGTATAAATTGTACGGACAAGACGTTA10.6369426751592357No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAC10.6369426751592357No Hit
ATCGTGCAGTTGTTAACGTGGATTCCGCTAACGTCTTCCTCGTTCTTGTAC10.6369426751592357No Hit
GTGTTCTTTACGTTGAATATCGAACTCGTAAAGAACGAGAAAAATTCGATT10.6369426751592357No Hit
TAACATAACGATGATTAAACGTACATCCAGTATGCATATCGACTTTATGAT10.6369426751592357No Hit
ATACAGTGGAGCACCCCAAAGAAGACGAGGTTTTGCAGTATGCCAGAGCTG10.6369426751592357No Hit
AACTGAGGAAGATGATTTGATTGGAACTGAATTGCAAGGACCAAGTGCTGT10.6369426751592357No Hit
GGGTGGAAGTCACAGCGATGACAAAGAGAAGCCGGGAAAAAACCCAGAGGG10.6369426751592357No Hit
GTATCGAGTATTCGTGTTACTGGTGTTTGTTAGTCAGCTTTGCAATGGGTT10.6369426751592357No Hit
GAATTGGATATATGGCCCAATTCCAACAAAGACGATATTGATGCTGTCTAT10.6369426751592357No Hit
CAGCATGTGAATAATGTAAAGTACATTGGGTCTGTCTCTTATACATATCTC10.6369426751592357No Hit
GAAGTAGGCATCAGTACAGCGATAGTCCTTATGTACAGCTTTATACCTGTC10.6369426751592357No Hit
GTTGTTATGTAATACAATTTGGTTCTTGCAAGAATACACAATCGTGGGACT10.6369426751592357No Hit
CAGTAAATGAACCAAAGATGTTTCGTATGTAGAGTAATGTGTATGGAAATG10.6369426751592357No Hit
GCTTCAATCCTTCGACAACATTAACCATTTTTGCATCATACATCTCTTGAA10.6369426751592357No Hit
CCTCTGGACTCTTCTCGATCGAGAGAAAAAGATGTGAGATGTCAACCATAT10.6369426751592357No Hit
GAATCTCACAGGAACAGAACCAGATTTGTCTGTCTCTTATACACATCCCGA10.6369426751592357No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.6369426751592357No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTTCGCGACTT10.6369426751592357No Hit
ACATAAACCCATGTCTTGTCCAATTGATTTTTGTCAGTTGCTGATTTATAG10.6369426751592357No Hit
AATATAGATTGCATTCGAAGATAGATGAGGATGAGAAGAATCTAGTTTCTA10.6369426751592357No Hit
CACTTCTTGAATACTTTCCTTTTTAAGACCAACGCTTTTCTCTGATTCTGT10.6369426751592357No Hit
CTTGTATGTTATCTATCACTTAGACCCCAATATTTTTGTTCCTGTCTCTTA10.6369426751592357No Hit
GTTTGAAGAAGGGAGGTGCCATCAGATATTCAAGGAGGAATGAGAACTGTG10.6369426751592357No Hit
ATGTCAATGTGAGGATACCTCTTTTGGATTGTGCTTCATCACCAACATACG10.6369426751592357No Hit
AGGTTATATGGTGTGACGGGTTTGAGTGGATAATAACATGGGATAGTATGC10.6369426751592357No Hit
CTCTAATTCTTACAAGTTTTGAAACTGTGTTTAACTGTTAAACAGAAAACA10.6369426751592357No Hit
ACATTATTCCAGAGTATCAGACGGAGGAAAGCATGTATAATTCTGTTAGGA10.6369426751592357No Hit
CAATTACCAAGATAGATCATGACAACTAGTAAGTTAATCAAATGCAAAGTG10.6369426751592357No Hit
CCAAAGGATAAGTACAACAACAACAACAACAATAATGGATACACTTACACT10.6369426751592357No Hit
TCTTTGAAGAGTCTAAAACTCCAGAAGACAAGATTTTGTTCATCCATGCTG10.6369426751592357No Hit
ACATAGGACTGTGGGTCTGCGTCGTGCTGTGCTGTGCCAGGCTATGCTGAG10.6369426751592357No Hit
CACCACTGCTCCGGCGACTTTTGCTTATCTTCCACCGTTTTCTCTTCTTCT10.6369426751592357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTTCTTTG10.6369426751592357No Hit
CTTCTCATTCTTCTTATTAGTTTTACTCTTCTCCGATACCTGTCTCTTATC10.6369426751592357No Hit
CGCCTGATGCTGTCGGAGAGCCTGGCTCCCGCCAGCCGCATCTTCGGCATG10.6369426751592357No Hit
ACCCATCACACCAATACATAGAGAACGACATTGTTGTTATCTAGATACATT10.6369426751592357No Hit
GAGTTCTGGCAGCCTACCCTCGAGGACACGCTTGACCCTGTCTCTTATACA10.6369426751592357No Hit
CAGCGAGGCTTCTTGGAAGTTTGAGTTTGCCAAATTCAGCAGCAGGCATGG10.6369426751592357No Hit
GTCCTTGATTGTTGTTTTCCAAAGGATCGAACAATGTTGTCTCGTAATGCT10.6369426751592357No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACA10.6369426751592357Illumina PCR Primer Index 5 (96% over 28bp)
ATAAAGTATTGTGCAGAGACATCAGTTAATTCTTTGGTTTTACTTTGTCTC10.6369426751592357No Hit
GGCACATCGAGCGCCAGCAGGTCGCGATCACCGGGCGGCGGCGGCTGGTTG10.6369426751592357No Hit
AGCTTACTGTCTTTGCTCTTTAGTTTTTCCCTTTTAGCCGGAAGTTCTTCT10.6369426751592357No Hit
GATCAATTTTAAAGTAAACCTAAATTCTACATACTCCAACACTGTCAAGTC10.6369426751592357No Hit
GTATTTTAGCATCTAGTTTGTCGGAGATAGAAACAAGTTTTAGCAAAACTA10.6369426751592357No Hit
GTGGGCAGGCGTGTTGGCACCCTGGTAGATGATGCCGCCGTCGAATTCCTG10.6369426751592357No Hit
TCTTAATGGTGCTACTGGAGAATGTGGCCTTCTCTTAACCTCTCTATGGTG10.6369426751592357No Hit
GAATAGAGAAATACTTTCAGATGATCATTAGCCCACATACCCTGTAACTTA10.6369426751592357No Hit
CCTCTGTTCCTAGAGGGACAAGATTGGCTGCTATTAGGGATGCTTGTGATC10.6369426751592357No Hit
GGGCGTTTAGATGTGGAAAAACCTTACTCCTGCCGCAACGCCCCAAACAGA10.6369426751592357No Hit
CTTATACACATCTACGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC10.6369426751592357No Hit
ACACCATGGTGCATAAAATTCGACTAGGACATCTTTGTTTTGATCCAGAAC10.6369426751592357No Hit
ATGTTGTATAGTCCTACTAGCAGCAATGCTGTTGTTGCCATTCTTGAGGAT10.6369426751592357No Hit
GTAGATGTTAACATTGGTGCCAATCCATCTGCTGAAGAGGTGGTGAGGATG10.6369426751592357No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT10.6369426751592357No Hit
TCTCACATCTAATACCGGTTTACAAGAACCCTGTCTTTATACACATCTCCG10.6369426751592357No Hit
GTACAAACTTCATTCACCATTATCCTGTAACACCTGCGATTTAGGGACTGT10.6369426751592357No Hit
CAAGCAATACAGTGGAACAAACAAAGACGACATAAAAATAAATGCAAGTAA10.6369426751592357No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers