FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_70.3410000000b15d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_70.3410000000b15d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052977
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC12670.12032551518219296No Hit
TTATTATGCCAAGCAAGTCAAACACATGCTTGTTGATTGAAATCAAGCTAT12020.11415254084372213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1700.034.41045845
GCCCACG1750.033.42730318
TATGCCG1750.033.42730343
GTATGCC1900.030.78830342
CGAGACT2050.029.63302223
CCGAGCC2000.029.2488914
ATGCCGT2250.027.99893644
GTCCGAA755.6403223E-826.99897612
TCCGAGC2200.026.589913
GCCCAAC856.4828782E-926.46958413
CGTATGC2300.026.4120441
AGCCCAC2300.025.43381717
GAGCCCA2300.025.43381716
CGAGCCC2300.025.43381715
GGGCGTC901.1912562E-824.99905238
GGTCCGA551.3684492E-424.54452311
CTCGTAT2400.024.37407339
CCACGAG2400.024.37407320
CTAGGCT1052.3665052E-923.5705344
CCCACGA2550.022.94030619
Produced by FastQC (version 0.11.2)