FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_69.3410000000b140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_69.3410000000b140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405961
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC41820.2974477954936161No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5750.037.17388545
ATGCCGT5950.035.9243444
TATGCCG6100.035.04095543
GCCCACG6100.035.03970718
CCCACGA6300.033.92733419
CGTATGC6600.032.38518541
CGCTATG6600.032.38518530
CCGAGCC6800.031.43268214
CTCGCTA6750.031.33219528
GTATGCC6850.031.20324542
ACTCGCT7050.029.9989127
CGAGCCC7200.029.99890915
CCACGAG7200.029.99890920
TCGCGCG300.005148560729.9989099
CGAGACT7150.029.57934623
CTCCGAG7350.029.08057412
TCGCTAT7300.028.9715529
TCCGAGC7400.028.88408713
ACGAGAC7500.028.79895422
TCGTATG7600.028.4200240
Produced by FastQC (version 0.11.2)