FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_67.3410000000b126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_67.3410000000b126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746523
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2350.033.50925418
CTACCGC2450.032.1415328
ATGCCGT2350.031.5953444
TGCCGTC2450.030.30573845
CGTATGC2550.029.99875641
CGAGCCC2550.029.99875615
TATGCCG2650.029.71660243
ACTACCG2850.028.41987827
CCCACGA3050.026.55627819
TACCGCT2950.025.93113129
TCCGAGC3200.024.60835813
GTCCCCT551.3689844E-424.5444429
CCGAGCC3250.023.53748914
AGCCCAC3350.022.83487717
ACCGCTG3350.022.83487730
GTATGCC3400.022.4990742
GACTACC3600.021.2491226
CCGCTGA3700.020.67482231
CTCCGAG3850.020.453712
CGCTGAT3850.020.453732
Produced by FastQC (version 0.11.2)